SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP207888
Component protein type	Tae4
T6SS ID (Type)	T6SS13923 (Type i3)
Strain	Enterobacter sp. DSM 30060
Replicon	chromosome
Sequence	Protein sequence (165 a.a.); Nucleotide sequence (495 bp)
Reference	[1] Flaugnatti N, Isaac S, Lemos Rocha LF, et al. Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms. Nat Commun. 2021 Oct 1;12(1):5751. doi: 10.1038/s41467-021-26041-0. PMID: 34599171

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	HWQ15_RS21385
Coordinate (Strand)	4485175..4485669 (-)
NCBI ID	WP_022648411.1
RefSeq	NZ_CP056118
Uniprot ID	A0A0A6FGP4_9ENTR, A0A5Q5DZK1_9ENTR, A0A7H9JU33_ENTCL
KEGG ID	ecli:ECNIH5_12500
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Tae4	PF14113.8	4.6e-15	46..161

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-164	MRPLYEQLKRQHYSSNHTRAGFASGEQVFSEIGYDYSTIIKQQPAYENTCAVRMSLALLKCNVEFTGRLPIKTGPYKGKTIEPGAKLLADQLYKSHVFGKAEIFKDREAGARSMLGRKGVVFFNKIDGYGGGHIDLIEPLSNNSFICNSDCFPYCQEIWFWELK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9650	-	-

Protein sequence: 165 a.a.

						>T6CP207888 NZ_CP056118:c4485669-4485175 [Enterobacter sp. DSM 30060] [Tae4]
MRPLYEQLKRQHYSSNHTRAGFASGEQVFSEIGYDYSTIIKQQPAYENTCAVRMSLALLKCNVEFTGRLPIKTGPYKGKT
IEPGAKLLADQLYKSHVFGKAEIFKDREAGARSMLGRKGVVFFNKIDGYGGGHIDLIEPLSNNSFICNSDCFPYCQEIWF
WELK*					

Nucleotide sequence: 495 bp

						>T6CP207888 NZ_CP056118:c4485669-4485175 [Enterobacter sp. DSM 30060] [Tae4]
ATGAGACCATTATACGAACAACTTAAACGTCAGCATTACTCTTCAAATCACACACGCGCGGGTTTTGCCTCGGGTGAACA
GGTTTTTTCAGAAATTGGCTACGATTATTCGACGATTATCAAGCAACAACCGGCATACGAAAATACCTGTGCGGTAAGAA
TGAGCCTGGCATTGTTAAAATGCAATGTTGAATTTACAGGGCGTTTACCCATAAAGACCGGTCCCTATAAGGGAAAAACA
ATCGAGCCTGGCGCAAAATTACTTGCCGATCAGCTTTATAAATCCCATGTGTTTGGCAAGGCAGAAATCTTTAAAGACCG
CGAGGCTGGTGCACGCTCAATGTTGGGCCGAAAAGGTGTTGTTTTCTTCAATAAAATTGATGGGTATGGGGGTGGTCATA
TCGACCTTATCGAGCCTCTGAGTAATAATTCGTTTATATGTAACTCCGATTGCTTTCCGTACTGTCAGGAAATCTGGTTT
TGGGAATTAAAATAA					