Detailed information of component protein
Summary
Component ID | T6CP207861 |
Component protein type | TssM |
T6SS ID (Type) | T6SS13922 (Type i2) |
Strain | Enterobacter sp. DSM 30060 |
Replicon | chromosome |
Sequence | Protein sequence (1148 a.a.); Nucleotide sequence (3444 bp) |
Reference | - |
External database links
Locus tag (Gene) | HWQ15_RS20555 |
Coordinate (Strand) | 4298317..4301760 (-) |
NCBI ID | WP_080454252.1 |
RefSeq | NZ_CP056118 |
Uniprot ID | - |
KEGG ID | - |
PDB ID | - |
Pfam domain hit(s)
Domain | Pfam ID | E-value | Aligned region |
---|---|---|---|
IcmF-related | PF06761.14 | 4.2e-62 | 422..739 |
IcmF_C | PF06744.14 | 5.3e-21 | 998..1102 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
Prediction | Region | Sequence |
---|---|---|
Inside | 1-11 | MTQKKELQSAR |
Transmembrane helix | 12-34 | YGFWGYFILIIGLAGITAFLLFI |
Outside | 35-48 | NQDALLKYGLAYRQ |
Transmembrane helix | 49-71 | IMSVWGGVFCFLFLGTVCSPFYW |
Inside | 72-373 | RRKQRKKQRNYLPPDSSGAETKTNPDVMTTITDYTDLKQHLNLRYRVFWRRKVRLLLITGDEAAIEQLVPGLQQQQWLEGNRTVLIYGGSLASEPDREKYTALRKLRRGRPLDGIIRVLPESPKLTPHISDSDLRGLEKISELLRYSAPVWLWQLCSSHWSQGTRPEQAVGASFPPRAKEDDVIRQLELMLPALRAQGMSQVAENSSHDFLLRLSQHLNDGAIARWAQQLQPWFFVSQQRVPLRGLMFSLSEKKPAGMSAGTADAENYGSQRHALTLPATWQGIVDDCTRVRGRRVGMPWER |
Transmembrane helix | 374-396 | ALAWSLMAIISVWGGGMLLSFAV |
Outside | 397-1147 | NRSQIVSVAQQAHALVEHPSVSDYQLTALHALRNDAGRLLHHIQDGAPWYQRFGLNHDQQLLDALLPWYGVANNRLIRDPANEALKQKLIALVNSAPNSDQRTQMAKPGYDQLKAWLMMSRPDKADGAFYAQTMKTVQPTRLGISTGLWQSLSPDLWAFYITELPKQPLWKTTPDAQLVSQSRQVLLQQIGRRNAESTLYENMLKSVRRNFADVSLEDMTGGTDARRLFTTDEVVPGMFTRQAWEGGIQQAIEKAANSRRDEIDWVLSDSRKTVSSDLSPEALKARLTERYFTDFAGSWLSFLNSLQLNPANNIADVTDQLTLMSDVRQSPLIALMNTLAWQGQAGQKSEGLSDSIIKSAKDLVGGKDKPVIDQSASGPQGPLDETFGPLLTLMGKNNGSNVMSADNSLSLQTYLTRITRVRLRLQQVASASDPQEMMQSLAQTVFQGKSVDLTDTQQYGSLIAASLGEEWSGFGSTMFVQPLTQAWETVLQPSAASLNDKWSRSVVANWHTAFDGRFPFAVSKSDSSLPMLAEFVRKDSGRIERFLTTELGGVLHKEGSQWVPDKVNSQGLNFNPAFLRAINQLSQLSDILFTDGSQGISFELQARPVPQVVETQLTIDGQKLHYFNQMADWQSFRWPGDTYKPGTMLTWTTVNAGARLFGDYSGTWGFIRWLDESKREQLDRSQWMMSFTAPDGRTLQWVLRSQLGSGPLALLALRGFTLPDQIFSVDSAAMAQALMTNTENSDMDGVE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction | Probability | Cleavage site | Signal peptide sequence |
---|---|---|---|
Other | 0.9978 | - | - |
Protein sequence: 1148 a.a.
>T6CP207861 NZ_CP056118:c4301760-4298317 [Enterobacter sp. DSM 30060] [TssM]
MTQKKELQSARYGFWGYFILIIGLAGITAFLLFINQDALLKYGLAYRQIMSVWGGVFCFLFLGTVCSPFYWRRKQRKKQR
NYLPPDSSGAETKTNPDVMTTITDYTDLKQHLNLRYRVFWRRKVRLLLITGDEAAIEQLVPGLQQQQWLEGNRTVLIYGG
SLASEPDREKYTALRKLRRGRPLDGIIRVLPESPKLTPHISDSDLRGLEKISELLRYSAPVWLWQLCSSHWSQGTRPEQA
VGASFPPRAKEDDVIRQLELMLPALRAQGMSQVAENSSHDFLLRLSQHLNDGAIARWAQQLQPWFFVSQQRVPLRGLMFS
LSEKKPAGMSAGTADAENYGSQRHALTLPATWQGIVDDCTRVRGRRVGMPWERALAWSLMAIISVWGGGMLLSFAVNRSQ
IVSVAQQAHALVEHPSVSDYQLTALHALRNDAGRLLHHIQDGAPWYQRFGLNHDQQLLDALLPWYGVANNRLIRDPANEA
LKQKLIALVNSAPNSDQRTQMAKPGYDQLKAWLMMSRPDKADGAFYAQTMKTVQPTRLGISTGLWQSLSPDLWAFYITEL
PKQPLWKTTPDAQLVSQSRQVLLQQIGRRNAESTLYENMLKSVRRNFADVSLEDMTGGTDARRLFTTDEVVPGMFTRQAW
EGGIQQAIEKAANSRRDEIDWVLSDSRKTVSSDLSPEALKARLTERYFTDFAGSWLSFLNSLQLNPANNIADVTDQLTLM
SDVRQSPLIALMNTLAWQGQAGQKSEGLSDSIIKSAKDLVGGKDKPVIDQSASGPQGPLDETFGPLLTLMGKNNGSNVMS
ADNSLSLQTYLTRITRVRLRLQQVASASDPQEMMQSLAQTVFQGKSVDLTDTQQYGSLIAASLGEEWSGFGSTMFVQPLT
QAWETVLQPSAASLNDKWSRSVVANWHTAFDGRFPFAVSKSDSSLPMLAEFVRKDSGRIERFLTTELGGVLHKEGSQWVP
DKVNSQGLNFNPAFLRAINQLSQLSDILFTDGSQGISFELQARPVPQVVETQLTIDGQKLHYFNQMADWQSFRWPGDTYK
PGTMLTWTTVNAGARLFGDYSGTWGFIRWLDESKREQLDRSQWMMSFTAPDGRTLQWVLRSQLGSGPLALLALRGFTLPD
QIFSVDSAAMAQALMTNTENSDMDGVE*
MTQKKELQSARYGFWGYFILIIGLAGITAFLLFINQDALLKYGLAYRQIMSVWGGVFCFLFLGTVCSPFYWRRKQRKKQR
NYLPPDSSGAETKTNPDVMTTITDYTDLKQHLNLRYRVFWRRKVRLLLITGDEAAIEQLVPGLQQQQWLEGNRTVLIYGG
SLASEPDREKYTALRKLRRGRPLDGIIRVLPESPKLTPHISDSDLRGLEKISELLRYSAPVWLWQLCSSHWSQGTRPEQA
VGASFPPRAKEDDVIRQLELMLPALRAQGMSQVAENSSHDFLLRLSQHLNDGAIARWAQQLQPWFFVSQQRVPLRGLMFS
LSEKKPAGMSAGTADAENYGSQRHALTLPATWQGIVDDCTRVRGRRVGMPWERALAWSLMAIISVWGGGMLLSFAVNRSQ
IVSVAQQAHALVEHPSVSDYQLTALHALRNDAGRLLHHIQDGAPWYQRFGLNHDQQLLDALLPWYGVANNRLIRDPANEA
LKQKLIALVNSAPNSDQRTQMAKPGYDQLKAWLMMSRPDKADGAFYAQTMKTVQPTRLGISTGLWQSLSPDLWAFYITEL
PKQPLWKTTPDAQLVSQSRQVLLQQIGRRNAESTLYENMLKSVRRNFADVSLEDMTGGTDARRLFTTDEVVPGMFTRQAW
EGGIQQAIEKAANSRRDEIDWVLSDSRKTVSSDLSPEALKARLTERYFTDFAGSWLSFLNSLQLNPANNIADVTDQLTLM
SDVRQSPLIALMNTLAWQGQAGQKSEGLSDSIIKSAKDLVGGKDKPVIDQSASGPQGPLDETFGPLLTLMGKNNGSNVMS
ADNSLSLQTYLTRITRVRLRLQQVASASDPQEMMQSLAQTVFQGKSVDLTDTQQYGSLIAASLGEEWSGFGSTMFVQPLT
QAWETVLQPSAASLNDKWSRSVVANWHTAFDGRFPFAVSKSDSSLPMLAEFVRKDSGRIERFLTTELGGVLHKEGSQWVP
DKVNSQGLNFNPAFLRAINQLSQLSDILFTDGSQGISFELQARPVPQVVETQLTIDGQKLHYFNQMADWQSFRWPGDTYK
PGTMLTWTTVNAGARLFGDYSGTWGFIRWLDESKREQLDRSQWMMSFTAPDGRTLQWVLRSQLGSGPLALLALRGFTLPD
QIFSVDSAAMAQALMTNTENSDMDGVE*
Nucleotide sequence: 3444 bp
>T6CP207861 NZ_CP056118:c4301760-4298317 [Enterobacter sp. DSM 30060] [TssM]
ATGACCCAGAAAAAGGAATTGCAGTCGGCACGTTATGGATTTTGGGGGTATTTTATTTTGATTATTGGCCTTGCTGGTAT
AACTGCGTTTTTGCTGTTTATTAATCAGGATGCCTTGCTTAAGTATGGTCTGGCATATCGCCAAATTATGTCTGTATGGG
GTGGTGTGTTTTGTTTTTTATTTTTAGGCACTGTTTGCTCTCCTTTTTATTGGCGGAGGAAGCAAAGAAAAAAACAACGC
AATTATTTACCACCGGATTCCTCGGGGGCTGAAACTAAAACAAATCCTGATGTGATGACAACAATCACAGATTATACAGA
TTTAAAACAGCACCTTAATCTTCGTTATAGAGTCTTTTGGCGTCGCAAAGTCCGGTTATTATTAATCACCGGCGATGAAG
CCGCGATTGAACAACTGGTACCCGGCCTGCAACAGCAGCAGTGGCTTGAAGGCAACCGCACCGTGCTGATTTACGGCGGC
AGCCTGGCATCGGAGCCTGACCGCGAAAAATACACCGCGCTGCGTAAACTACGCCGCGGGCGCCCGCTGGACGGGATTAT
CCGTGTACTGCCTGAGTCGCCTAAGTTGACCCCACATATCAGCGACAGCGATTTACGCGGCCTGGAAAAAATCAGCGAAC
TGCTGCGTTATTCCGCTCCGGTCTGGCTGTGGCAACTGTGCAGCAGTCACTGGTCGCAGGGTACGCGTCCTGAGCAGGCG
GTAGGCGCAAGCTTTCCACCACGCGCAAAAGAAGATGATGTAATCCGCCAGCTGGAACTGATGCTGCCGGCCCTGCGGGC
GCAGGGGATGAGTCAGGTCGCGGAAAACAGCAGCCACGACTTCCTGCTGCGCCTGAGCCAGCACCTGAACGACGGTGCTA
TTGCCCGCTGGGCTCAGCAGTTGCAGCCATGGTTCTTTGTTTCTCAGCAGCGCGTCCCGCTGCGTGGTCTGATGTTCAGC
CTGTCGGAGAAGAAACCTGCCGGAATGTCAGCGGGAACAGCCGACGCTGAGAATTATGGATCACAGCGTCATGCCTTGAC
TCTGCCAGCAACCTGGCAGGGTATTGTTGACGACTGCACCCGCGTACGTGGCCGTCGTGTCGGTATGCCGTGGGAACGAG
CGCTGGCCTGGTCACTGATGGCCATCATCAGTGTCTGGGGGGGAGGGATGCTGCTGTCGTTTGCGGTCAACCGGTCACAA
ATAGTTTCTGTTGCACAGCAGGCTCATGCTCTGGTGGAGCACCCTTCTGTATCGGATTACCAGCTGACGGCCCTGCATGC
TTTGCGTAATGATGCCGGTCGCCTGCTGCACCATATTCAGGACGGCGCGCCATGGTATCAGCGCTTTGGTCTTAACCATG
ATCAGCAGTTGCTTGACGCATTGCTGCCCTGGTACGGGGTGGCGAACAACCGTCTGATACGTGATCCGGCAAATGAAGCG
CTTAAGCAGAAGCTCATTGCGCTGGTCAATTCGGCACCCAACAGCGACCAGCGGACACAGATGGCGAAACCAGGTTATGA
CCAGCTGAAAGCCTGGCTGATGATGTCCCGTCCGGATAAAGCTGATGGTGCATTCTACGCGCAAACCATGAAAACCGTGC
AGCCGACGCGTTTAGGTATCTCAACTGGCCTGTGGCAAAGCCTGTCACCGGATTTGTGGGCCTTCTACATCACCGAACTG
CCTAAGCAGCCACTGTGGAAAACCACACCGGATGCGCAACTGGTCAGCCAGAGCCGCCAGGTGTTGTTGCAGCAGATAGG
ACGGCGCAACGCGGAAAGTACGCTGTACGAGAACATGCTCAAATCCGTACGTCGTAACTTTGCCGATGTGTCTCTGGAGG
ACATGACTGGCGGTACCGATGCCCGTCGCCTGTTTACCACCGATGAGGTGGTGCCGGGCATGTTCACCCGTCAGGCCTGG
GAGGGAGGGATTCAGCAGGCCATTGAGAAAGCGGCAAACTCCCGCCGCGATGAAATCGACTGGGTACTGAGCGACAGCCG
GAAAACCGTTTCCTCAGACCTGTCGCCGGAAGCACTGAAAGCACGTCTGACGGAACGCTACTTCACCGACTTTGCCGGTA
GCTGGCTGAGCTTCCTCAACAGCCTGCAACTTAATCCGGCAAACAATATTGCAGACGTCACTGACCAGCTGACGCTGATG
AGTGATGTTCGCCAGTCACCGCTGATTGCCCTGATGAACACCCTCGCCTGGCAGGGGCAGGCCGGCCAGAAGAGTGAAGG
CCTATCGGACTCCATCATCAAATCGGCTAAAGACCTGGTAGGCGGGAAGGACAAACCGGTAATTGACCAGTCAGCCTCAG
GCCCGCAGGGCCCACTGGATGAAACCTTTGGACCGTTGCTAACACTTATGGGTAAAAACAACGGCAGCAACGTTATGTCG
GCTGACAATTCACTGAGCCTGCAAACGTATCTTACCCGTATCACCCGTGTGCGTCTGCGTCTGCAACAGGTCGCCAGCGC
CTCTGACCCTCAGGAAATGATGCAGAGCCTGGCGCAGACCGTATTCCAGGGCAAAAGCGTGGACCTGACCGACACCCAGC
AGTACGGCAGCCTGATTGCTGCAAGCCTGGGCGAAGAGTGGAGTGGTTTTGGCAGCACCATGTTTGTGCAGCCGCTGACC
CAGGCCTGGGAAACTGTGCTTCAGCCATCGGCAGCGAGCCTGAATGACAAATGGAGTCGTTCCGTGGTGGCGAACTGGCA
TACGGCATTTGACGGACGTTTCCCATTTGCTGTCAGCAAGAGTGATTCCTCTCTGCCAATGCTGGCTGAGTTTGTGCGTA
AGGACAGCGGACGCATTGAGCGCTTCCTGACCACGGAACTCGGCGGTGTGCTGCATAAGGAGGGCAGCCAGTGGGTACCG
GATAAGGTCAACAGCCAGGGACTGAACTTTAACCCAGCCTTCCTGCGGGCGATTAATCAGCTCAGCCAGCTATCGGATAT
CTTGTTTACCGACGGCAGTCAGGGCATCAGCTTTGAACTACAGGCGCGTCCGGTACCGCAGGTGGTAGAAACTCAACTAA
CTATTGATGGACAAAAGCTACACTACTTCAACCAGATGGCAGACTGGCAGAGCTTCCGCTGGCCTGGTGACACCTACAAG
CCCGGAACCATGCTGACATGGACCACCGTCAATGCGGGTGCACGTCTGTTTGGTGATTACAGCGGAACCTGGGGCTTTAT
TCGCTGGCTGGATGAGAGTAAACGAGAGCAGCTTGACCGCAGTCAGTGGATGATGAGTTTTACCGCGCCGGATGGTCGTA
CCCTACAGTGGGTACTGCGTTCACAACTGGGCAGTGGGCCGCTGGCGTTACTGGCACTGCGGGGCTTCACGCTGCCAGAT
CAAATATTTAGCGTAGATAGCGCGGCTATGGCGCAGGCGCTGATGACCAATACAGAAAACAGTGACATGGACGGAGTCGA
ATAA
ATGACCCAGAAAAAGGAATTGCAGTCGGCACGTTATGGATTTTGGGGGTATTTTATTTTGATTATTGGCCTTGCTGGTAT
AACTGCGTTTTTGCTGTTTATTAATCAGGATGCCTTGCTTAAGTATGGTCTGGCATATCGCCAAATTATGTCTGTATGGG
GTGGTGTGTTTTGTTTTTTATTTTTAGGCACTGTTTGCTCTCCTTTTTATTGGCGGAGGAAGCAAAGAAAAAAACAACGC
AATTATTTACCACCGGATTCCTCGGGGGCTGAAACTAAAACAAATCCTGATGTGATGACAACAATCACAGATTATACAGA
TTTAAAACAGCACCTTAATCTTCGTTATAGAGTCTTTTGGCGTCGCAAAGTCCGGTTATTATTAATCACCGGCGATGAAG
CCGCGATTGAACAACTGGTACCCGGCCTGCAACAGCAGCAGTGGCTTGAAGGCAACCGCACCGTGCTGATTTACGGCGGC
AGCCTGGCATCGGAGCCTGACCGCGAAAAATACACCGCGCTGCGTAAACTACGCCGCGGGCGCCCGCTGGACGGGATTAT
CCGTGTACTGCCTGAGTCGCCTAAGTTGACCCCACATATCAGCGACAGCGATTTACGCGGCCTGGAAAAAATCAGCGAAC
TGCTGCGTTATTCCGCTCCGGTCTGGCTGTGGCAACTGTGCAGCAGTCACTGGTCGCAGGGTACGCGTCCTGAGCAGGCG
GTAGGCGCAAGCTTTCCACCACGCGCAAAAGAAGATGATGTAATCCGCCAGCTGGAACTGATGCTGCCGGCCCTGCGGGC
GCAGGGGATGAGTCAGGTCGCGGAAAACAGCAGCCACGACTTCCTGCTGCGCCTGAGCCAGCACCTGAACGACGGTGCTA
TTGCCCGCTGGGCTCAGCAGTTGCAGCCATGGTTCTTTGTTTCTCAGCAGCGCGTCCCGCTGCGTGGTCTGATGTTCAGC
CTGTCGGAGAAGAAACCTGCCGGAATGTCAGCGGGAACAGCCGACGCTGAGAATTATGGATCACAGCGTCATGCCTTGAC
TCTGCCAGCAACCTGGCAGGGTATTGTTGACGACTGCACCCGCGTACGTGGCCGTCGTGTCGGTATGCCGTGGGAACGAG
CGCTGGCCTGGTCACTGATGGCCATCATCAGTGTCTGGGGGGGAGGGATGCTGCTGTCGTTTGCGGTCAACCGGTCACAA
ATAGTTTCTGTTGCACAGCAGGCTCATGCTCTGGTGGAGCACCCTTCTGTATCGGATTACCAGCTGACGGCCCTGCATGC
TTTGCGTAATGATGCCGGTCGCCTGCTGCACCATATTCAGGACGGCGCGCCATGGTATCAGCGCTTTGGTCTTAACCATG
ATCAGCAGTTGCTTGACGCATTGCTGCCCTGGTACGGGGTGGCGAACAACCGTCTGATACGTGATCCGGCAAATGAAGCG
CTTAAGCAGAAGCTCATTGCGCTGGTCAATTCGGCACCCAACAGCGACCAGCGGACACAGATGGCGAAACCAGGTTATGA
CCAGCTGAAAGCCTGGCTGATGATGTCCCGTCCGGATAAAGCTGATGGTGCATTCTACGCGCAAACCATGAAAACCGTGC
AGCCGACGCGTTTAGGTATCTCAACTGGCCTGTGGCAAAGCCTGTCACCGGATTTGTGGGCCTTCTACATCACCGAACTG
CCTAAGCAGCCACTGTGGAAAACCACACCGGATGCGCAACTGGTCAGCCAGAGCCGCCAGGTGTTGTTGCAGCAGATAGG
ACGGCGCAACGCGGAAAGTACGCTGTACGAGAACATGCTCAAATCCGTACGTCGTAACTTTGCCGATGTGTCTCTGGAGG
ACATGACTGGCGGTACCGATGCCCGTCGCCTGTTTACCACCGATGAGGTGGTGCCGGGCATGTTCACCCGTCAGGCCTGG
GAGGGAGGGATTCAGCAGGCCATTGAGAAAGCGGCAAACTCCCGCCGCGATGAAATCGACTGGGTACTGAGCGACAGCCG
GAAAACCGTTTCCTCAGACCTGTCGCCGGAAGCACTGAAAGCACGTCTGACGGAACGCTACTTCACCGACTTTGCCGGTA
GCTGGCTGAGCTTCCTCAACAGCCTGCAACTTAATCCGGCAAACAATATTGCAGACGTCACTGACCAGCTGACGCTGATG
AGTGATGTTCGCCAGTCACCGCTGATTGCCCTGATGAACACCCTCGCCTGGCAGGGGCAGGCCGGCCAGAAGAGTGAAGG
CCTATCGGACTCCATCATCAAATCGGCTAAAGACCTGGTAGGCGGGAAGGACAAACCGGTAATTGACCAGTCAGCCTCAG
GCCCGCAGGGCCCACTGGATGAAACCTTTGGACCGTTGCTAACACTTATGGGTAAAAACAACGGCAGCAACGTTATGTCG
GCTGACAATTCACTGAGCCTGCAAACGTATCTTACCCGTATCACCCGTGTGCGTCTGCGTCTGCAACAGGTCGCCAGCGC
CTCTGACCCTCAGGAAATGATGCAGAGCCTGGCGCAGACCGTATTCCAGGGCAAAAGCGTGGACCTGACCGACACCCAGC
AGTACGGCAGCCTGATTGCTGCAAGCCTGGGCGAAGAGTGGAGTGGTTTTGGCAGCACCATGTTTGTGCAGCCGCTGACC
CAGGCCTGGGAAACTGTGCTTCAGCCATCGGCAGCGAGCCTGAATGACAAATGGAGTCGTTCCGTGGTGGCGAACTGGCA
TACGGCATTTGACGGACGTTTCCCATTTGCTGTCAGCAAGAGTGATTCCTCTCTGCCAATGCTGGCTGAGTTTGTGCGTA
AGGACAGCGGACGCATTGAGCGCTTCCTGACCACGGAACTCGGCGGTGTGCTGCATAAGGAGGGCAGCCAGTGGGTACCG
GATAAGGTCAACAGCCAGGGACTGAACTTTAACCCAGCCTTCCTGCGGGCGATTAATCAGCTCAGCCAGCTATCGGATAT
CTTGTTTACCGACGGCAGTCAGGGCATCAGCTTTGAACTACAGGCGCGTCCGGTACCGCAGGTGGTAGAAACTCAACTAA
CTATTGATGGACAAAAGCTACACTACTTCAACCAGATGGCAGACTGGCAGAGCTTCCGCTGGCCTGGTGACACCTACAAG
CCCGGAACCATGCTGACATGGACCACCGTCAATGCGGGTGCACGTCTGTTTGGTGATTACAGCGGAACCTGGGGCTTTAT
TCGCTGGCTGGATGAGAGTAAACGAGAGCAGCTTGACCGCAGTCAGTGGATGATGAGTTTTACCGCGCCGGATGGTCGTA
CCCTACAGTGGGTACTGCGTTCACAACTGGGCAGTGGGCCGCTGGCGTTACTGGCACTGCGGGGCTTCACGCTGCCAGAT
CAAATATTTAGCGTAGATAGCGCGGCTATGGCGCAGGCGCTGATGACCAATACAGAAAACAGTGACATGGACGGAGTCGA
ATAA