Detailed information of component protein
Summary
| Component ID | T6CP207857 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS13922  (Type i2) |
| Strain | Enterobacter sp. DSM 30060 |
| Replicon | chromosome |
| Sequence | Protein sequence (182 a.a.); Nucleotide sequence (546 bp) |
| Reference | - |
Bioinformatics investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | HWQ15_RS20535 |
| Coordinate (Strand) | 4293348..4293893 (-) |
| NCBI ID | WP_032624166.1 |
| RefSeq | NZ_CP056118 |
| Uniprot ID | - |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 4.9e-30 | 49..170 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-181 | VLRTSSIKHASLLPALLAFSLTGCGVTQGITDGTKSAFNSVFYKKIKMLHLDFTAREALNTDSRENNSLSEPVVVRVYQLKDRKIFDKTVYQQLLKDGETILKADLLATRDVVVKPGGDTNLDMPMEEETQFVAVVGLFRHPDLDNNTWKQVLKREDLDPDKPRILEAGNNHLTLQPLKDD |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9981 | pos: 23-24. LTG-CG. | VLRTSSIKHASLLPALLAFSLTG |
Protein sequence: 182 a.a.
>T6CP207857 NZ_CP056118:c4293893-4293348 [Enterobacter sp. DSM 30060] [TssJ]
VLRTSSIKHASLLPALLAFSLTGCGVTQGITDGTKSAFNSVFYKKIKMLHLDFTAREALNTDSRENNSLSEPVVVRVYQL
KDRKIFDKTVYQQLLKDGETILKADLLATRDVVVKPGGDTNLDMPMEEETQFVAVVGLFRHPDLDNNTWKQVLKREDLDP
DKPRILEAGNNHLTLQPLKDD*
VLRTSSIKHASLLPALLAFSLTGCGVTQGITDGTKSAFNSVFYKKIKMLHLDFTAREALNTDSRENNSLSEPVVVRVYQL
KDRKIFDKTVYQQLLKDGETILKADLLATRDVVVKPGGDTNLDMPMEEETQFVAVVGLFRHPDLDNNTWKQVLKREDLDP
DKPRILEAGNNHLTLQPLKDD*
Nucleotide sequence: 546 bp
>T6CP207857 NZ_CP056118:c4293893-4293348 [Enterobacter sp. DSM 30060] [TssJ]
GTGTTACGTACAAGTTCAATTAAACATGCGAGCTTGCTGCCTGCTCTGCTGGCATTCAGCCTGACGGGGTGCGGGGTGAC
GCAGGGCATCACTGATGGCACCAAATCGGCATTTAACTCCGTGTTCTACAAAAAAATTAAGATGCTGCACCTGGATTTCA
CGGCGCGTGAAGCGTTAAACACCGATTCCCGCGAAAATAACTCACTCTCCGAGCCGGTGGTAGTCCGCGTCTATCAACTG
AAAGACCGTAAAATCTTCGATAAAACGGTTTACCAGCAACTCCTGAAAGATGGCGAAACCATCCTGAAAGCCGACCTGCT
GGCGACCCGAGATGTGGTCGTAAAACCGGGTGGTGATACAAATCTTGATATGCCAATGGAAGAAGAAACGCAGTTTGTCG
CGGTAGTCGGCCTGTTTCGTCACCCGGACCTGGACAACAATACCTGGAAGCAGGTTCTAAAACGTGAAGATCTCGATCCG
GATAAGCCGCGCATTCTGGAGGCCGGGAATAACCACCTGACGTTACAGCCATTGAAGGATGACTGA
GTGTTACGTACAAGTTCAATTAAACATGCGAGCTTGCTGCCTGCTCTGCTGGCATTCAGCCTGACGGGGTGCGGGGTGAC
GCAGGGCATCACTGATGGCACCAAATCGGCATTTAACTCCGTGTTCTACAAAAAAATTAAGATGCTGCACCTGGATTTCA
CGGCGCGTGAAGCGTTAAACACCGATTCCCGCGAAAATAACTCACTCTCCGAGCCGGTGGTAGTCCGCGTCTATCAACTG
AAAGACCGTAAAATCTTCGATAAAACGGTTTACCAGCAACTCCTGAAAGATGGCGAAACCATCCTGAAAGCCGACCTGCT
GGCGACCCGAGATGTGGTCGTAAAACCGGGTGGTGATACAAATCTTGATATGCCAATGGAAGAAGAAACGCAGTTTGTCG
CGGTAGTCGGCCTGTTTCGTCACCCGGACCTGGACAACAATACCTGGAAGCAGGTTCTAAAACGTGAAGATCTCGATCCG
GATAAGCCGCGCATTCTGGAGGCCGGGAATAACCACCTGACGTTACAGCCATTGAAGGATGACTGA