Detailed information of component protein


Summary

Component ID T6CP016248
Component protein type TssM
T6SS ID (Type) T6SS01301 experimental (Type i3)
Strain Burkholderia gladioli strain NGJ1
Replicon chromosome
Sequence Protein sequence (1202 a.a.); Nucleotide sequence (3606 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) ACI79_RS29685
Coordinate (Strand) 75443..79048 (+)
NCBI ID 836691993
GenBank LEKY01000074
Uniprot ID UPI00064944AC
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 3.5e-90 193..450
IcmF-related PF06761.14 8.7e-58 505..803
IcmF_C PF06744.14 4.2e-12 1077..1182
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 MNLLKTWVRSRW
Transmembrane helix 13-35 FASAVGLALIAVLIWLGGPYLGL
Outside 36-44 GDRQPLASP
Transmembrane helix 45-67 AARLALILALVLAWLVVLLVLQW
Inside 68-440 RAQRKTARLSGELAGQEAKLAAEEPGSAERAALQQRFREAIDTLRRTRRNGRNLYALPWYVVIGPPGSGKSTLLQNSGLDFPLSERFGNKALRGIGGTRDCDWWFTDEAVFLDTAGRYTTQDSDAAADASAWQAFLTLLRRYRRRRPLNGVIVTMSVSDLLSFDETARQQHMRAVRRRLDELTQYLKVPVPVYLVFTKSDLVAGFGEFFDDLGPELRAQVWGMTFPLEASLDGTACSRFAGEYDLLLERLNTRLIERLHAERDRGRRAAVLSFPQQLGALREIARQFVEGTFGRNEYGTPPLLRGAYLSSGTQEGAPIDRMLGAVARTFGVDAARMQPGTSQRRTFFVERLLKEVLFRESGLAGTNPRLERQK
Transmembrane helix 441-463 LLVQIAAYAGITLFTVLMVAGFA
Outside 464-1201 TSYARNRAYVAEVQRSLKDLPGADTLAGQPDLRSYFARALARLEVISDTQDSAGRYRDHVPLLMRFGLFQGRALHAQAHGAYLRELDGTLLPGVGLRFREGLTDSANDPQTLYGYLKGYLMLGEPQHLVPDELAALARQEWQRLFPDDPAIRKALDKHFTARVEDPQRPRALSLDSGLVDQARATLKTADLATLIYGSVKLDADRSGVSPVRLDESLGLLGNVFRRKSGTPLAQPLPVLFTRPYFAYEVNGGLDRSVDGFVKDYWVFDTGRVDPLARSRYAQQVLTLYEQDYIRNWDGLLGDLQLQPVLSIQDASALAAKLSGPSSPLKALLNLVRDNTSDLLRSGPAGANGASGVAGVAGVSGAEAGEQAKTIGENIAARRALNTRLAGELRTAGAPLPYGVTLASRNHGGGQDAGGQPGAAIEAHFAQIDQLSLGSPGATPLDHTIGVLDQLGKTLLTMNDLSDPSAQNNPALLSARQEAQQLPPQVAGLISGLTGKSADLVASGGSAALADQFRAAAGNDCASFVDGRYPFASGGATDIPLQNFTELFGGGGRFDAFFKSALAARVDTGGSAWRWKPNMPPGPSGVLAGAQLADEIRQLYFGGGAQVHVGFTLLAPQFDTAIARVVVEVDGQKYDFTANAASVASSSMSWPGPQPGHVVISAFDGSNHPVGTPYRFDGDWSLFHALDAGRLRKEGELRFTVSYDFAGHPVKLTIQPASLRNPFLNDAVRRFRCPR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9827 - -


  Protein sequence: 1202 a.a.    

>T6CP016248 LEKY01000074:75443-79048 [Burkholderia gladioli] [TssM]
MNLLKTWVRSRWFASAVGLALIAVLIWLGGPYLGLGDRQPLASPAARLALILALVLAWLVVLLVLQWRAQRKTARLSGEL
AGQEAKLAAEEPGSAERAALQQRFREAIDTLRRTRRNGRNLYALPWYVVIGPPGSGKSTLLQNSGLDFPLSERFGNKALR
GIGGTRDCDWWFTDEAVFLDTAGRYTTQDSDAAADASAWQAFLTLLRRYRRRRPLNGVIVTMSVSDLLSFDETARQQHMR
AVRRRLDELTQYLKVPVPVYLVFTKSDLVAGFGEFFDDLGPELRAQVWGMTFPLEASLDGTACSRFAGEYDLLLERLNTR
LIERLHAERDRGRRAAVLSFPQQLGALREIARQFVEGTFGRNEYGTPPLLRGAYLSSGTQEGAPIDRMLGAVARTFGVDA
ARMQPGTSQRRTFFVERLLKEVLFRESGLAGTNPRLERQKLLVQIAAYAGITLFTVLMVAGFATSYARNRAYVAEVQRSL
KDLPGADTLAGQPDLRSYFARALARLEVISDTQDSAGRYRDHVPLLMRFGLFQGRALHAQAHGAYLRELDGTLLPGVGLR
FREGLTDSANDPQTLYGYLKGYLMLGEPQHLVPDELAALARQEWQRLFPDDPAIRKALDKHFTARVEDPQRPRALSLDSG
LVDQARATLKTADLATLIYGSVKLDADRSGVSPVRLDESLGLLGNVFRRKSGTPLAQPLPVLFTRPYFAYEVNGGLDRSV
DGFVKDYWVFDTGRVDPLARSRYAQQVLTLYEQDYIRNWDGLLGDLQLQPVLSIQDASALAAKLSGPSSPLKALLNLVRD
NTSDLLRSGPAGANGASGVAGVAGVSGAEAGEQAKTIGENIAARRALNTRLAGELRTAGAPLPYGVTLASRNHGGGQDAG
GQPGAAIEAHFAQIDQLSLGSPGATPLDHTIGVLDQLGKTLLTMNDLSDPSAQNNPALLSARQEAQQLPPQVAGLISGLT
GKSADLVASGGSAALADQFRAAAGNDCASFVDGRYPFASGGATDIPLQNFTELFGGGGRFDAFFKSALAARVDTGGSAWR
WKPNMPPGPSGVLAGAQLADEIRQLYFGGGAQVHVGFTLLAPQFDTAIARVVVEVDGQKYDFTANAASVASSSMSWPGPQ
PGHVVISAFDGSNHPVGTPYRFDGDWSLFHALDAGRLRKEGELRFTVSYDFAGHPVKLTIQPASLRNPFLNDAVRRFRCP
R*

  Nucleotide sequence: 3606 bp    

>T6CP016248 LEKY01000074:75443-79048 [Burkholderia gladioli] [TssM]
ATGAACCTGCTGAAAACCTGGGTGCGGTCCCGGTGGTTCGCCTCGGCGGTCGGCCTCGCGCTGATCGCCGTGCTGATCTG
GCTCGGCGGCCCCTATCTCGGCCTCGGCGATCGCCAGCCGCTGGCCAGCCCCGCCGCGCGCCTGGCGCTGATCCTCGCGC
TGGTGCTGGCCTGGCTGGTGGTGCTGCTGGTGCTGCAATGGCGTGCCCAGCGCAAGACCGCGCGCCTGTCCGGCGAGTTG
GCCGGGCAGGAGGCGAAGCTGGCCGCCGAGGAGCCGGGCAGCGCCGAGCGCGCCGCCTTGCAACAGCGCTTTCGCGAGGC
GATCGACACGCTGCGCCGCACGCGCCGCAACGGCCGCAATCTCTACGCGCTGCCCTGGTACGTGGTGATCGGGCCGCCGG
GTTCGGGCAAGAGCACCCTGCTGCAGAATTCCGGGCTCGATTTCCCGCTGTCCGAGCGCTTCGGCAACAAGGCGCTGCGC
GGCATCGGCGGCACGCGCGACTGCGACTGGTGGTTCACCGACGAGGCCGTGTTCCTCGACACCGCCGGGCGCTACACCAC
GCAGGATTCCGACGCGGCCGCCGACGCCTCGGCCTGGCAGGCCTTCCTGACCCTGCTGCGCCGCTATCGCCGCCGCCGGC
CGCTGAACGGCGTGATCGTGACGATGAGCGTGTCGGACCTGCTGAGCTTCGACGAAACCGCGCGCCAGCAGCACATGCGC
GCGGTGCGCCGGCGCCTGGACGAACTCACGCAATACCTGAAGGTGCCGGTGCCGGTCTACCTGGTGTTCACCAAGAGCGA
CCTGGTGGCGGGCTTCGGCGAGTTCTTCGACGACCTCGGCCCCGAGCTGCGCGCCCAGGTCTGGGGCATGACCTTCCCGC
TCGAGGCCAGCCTCGACGGCACCGCCTGCAGCCGCTTCGCGGGCGAATACGACCTGCTGCTGGAGCGCCTCAACACGCGC
CTGATCGAGCGCCTGCACGCCGAGCGCGACCGCGGCCGGCGCGCCGCCGTGCTGAGCTTCCCGCAGCAGCTCGGCGCGTT
GCGCGAGATCGCGCGGCAGTTCGTGGAAGGCACCTTCGGGCGCAACGAATACGGCACGCCGCCGCTGCTGCGCGGCGCCT
ACCTCAGCTCGGGCACGCAGGAAGGCGCGCCGATCGATCGCATGCTCGGCGCGGTGGCGCGCACCTTCGGCGTCGATGCC
GCGCGCATGCAGCCCGGCACCAGCCAGCGCCGCACCTTCTTCGTCGAGCGCCTGCTCAAGGAGGTGCTGTTCCGCGAATC
GGGCCTGGCCGGCACCAACCCGCGCCTGGAGCGCCAGAAGCTGCTGGTCCAGATCGCCGCCTATGCCGGCATCACCCTGT
TCACGGTGCTGATGGTGGCCGGCTTCGCCACCAGCTACGCGCGCAACCGCGCCTACGTGGCCGAGGTGCAGCGCAGCCTG
AAGGACCTGCCCGGCGCCGACACGCTGGCCGGGCAGCCCGACCTGCGCAGCTATTTCGCGCGCGCGCTGGCGCGGCTGGA
GGTGATCTCGGACACGCAGGATTCGGCGGGCCGCTATCGCGATCATGTGCCGCTGCTGATGCGCTTCGGGCTGTTCCAGG
GCCGGGCGCTGCATGCGCAGGCGCATGGCGCCTACCTGCGCGAGCTCGACGGCACCTTGCTGCCCGGCGTCGGCCTGCGT
TTCCGCGAGGGGCTGACCGACAGCGCCAACGATCCGCAGACGCTGTACGGCTACCTGAAGGGCTACCTGATGCTCGGCGA
GCCGCAGCACCTGGTGCCCGACGAGCTGGCAGCGCTCGCGCGCCAGGAATGGCAGCGCCTGTTCCCCGACGATCCGGCGA
TCCGCAAGGCGCTCGACAAGCACTTCACGGCGCGCGTCGAGGATCCGCAGAGGCCGCGCGCGCTGTCTCTCGACAGCGGC
CTGGTGGACCAGGCCCGCGCCACGCTGAAGACGGCCGACCTCGCCACCCTGATCTACGGCAGCGTCAAGCTCGACGCCGA
TCGCTCCGGCGTCTCGCCGGTGCGGCTCGACGAATCGCTGGGCCTGCTCGGCAACGTGTTCCGTCGCAAGAGCGGCACGC
CGCTCGCGCAGCCGCTGCCGGTGCTGTTCACGCGGCCGTATTTCGCCTACGAGGTGAACGGCGGGCTCGATCGCTCGGTG
GACGGCTTCGTCAAGGACTACTGGGTGTTCGACACGGGCCGCGTCGATCCGCTGGCGCGCTCGCGTTACGCGCAGCAGGT
GCTCACGCTCTACGAGCAGGACTACATCCGCAACTGGGACGGCCTGCTCGGCGATCTGCAGCTGCAGCCGGTGCTCAGCA
TCCAGGATGCCAGCGCGCTGGCCGCGAAGCTGTCGGGGCCGAGCTCGCCGCTCAAGGCGCTGCTGAACCTGGTGCGCGAC
AACACCAGCGACCTGCTGCGCAGCGGGCCCGCCGGCGCCAACGGCGCGAGCGGCGTGGCCGGTGTGGCGGGTGTCTCGGG
CGCCGAGGCGGGCGAGCAGGCCAAGACCATCGGCGAGAACATCGCCGCGCGCCGCGCGCTCAACACCAGGCTGGCCGGTG
AGCTGCGCACGGCCGGCGCGCCGCTGCCCTATGGCGTGACGCTGGCCTCGCGCAACCACGGCGGCGGCCAGGACGCCGGC
GGCCAGCCGGGCGCGGCGATCGAGGCGCATTTCGCGCAGATCGACCAGCTGAGCCTGGGTTCGCCCGGCGCCACGCCGCT
CGATCACACGATCGGCGTGCTCGACCAGCTCGGCAAGACCCTGCTGACCATGAACGACCTCAGCGACCCGAGCGCGCAGA
ACAACCCGGCCCTGCTGTCGGCGCGCCAGGAAGCGCAGCAACTGCCGCCGCAGGTGGCGGGGCTGATCTCGGGCCTGACC
GGCAAGAGCGCCGACCTGGTGGCCAGCGGCGGCAGCGCCGCGCTGGCCGACCAGTTCCGCGCGGCGGCCGGCAACGACTG
CGCGAGCTTCGTCGACGGCCGCTACCCGTTCGCCTCGGGCGGCGCCACCGATATCCCGCTGCAGAACTTCACCGAGCTGT
TCGGCGGCGGCGGGCGCTTCGACGCCTTCTTCAAGTCGGCGCTGGCCGCACGCGTCGACACCGGCGGCAGCGCCTGGCGC
TGGAAGCCGAACATGCCGCCGGGCCCGAGCGGCGTGCTGGCCGGCGCGCAACTGGCCGACGAGATCCGCCAGCTCTACTT
CGGCGGCGGCGCCCAGGTGCATGTGGGCTTCACCCTGCTCGCGCCGCAGTTCGACACCGCGATCGCGCGCGTGGTGGTGG
AGGTGGACGGCCAGAAGTACGACTTCACGGCCAATGCCGCCAGCGTGGCCTCCAGCTCGATGAGCTGGCCCGGCCCGCAG
CCCGGCCATGTGGTGATCAGCGCCTTCGACGGCTCGAACCACCCGGTCGGCACGCCCTACAGGTTCGACGGCGACTGGTC
GCTGTTCCATGCGCTCGACGCGGGGCGCCTGCGGAAGGAGGGCGAGCTGCGCTTCACGGTCAGCTACGATTTCGCCGGCC
ATCCCGTGAAGCTGACGATCCAGCCCGCCAGCCTGCGCAACCCCTTCCTCAATGACGCGGTGCGTCGCTTCAGGTGCCCG
CGATGA