Detailed information of component protein


Summary

Component ID T6CP016247
Component protein type TssL
T6SS ID (Type) T6SS01301 experimental (Type i3)
Strain Burkholderia gladioli strain NGJ1
Replicon chromosome
Sequence Protein sequence (469 a.a.); Nucleotide sequence (1407 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) ACI79_RS29680
Coordinate (Strand) 74032..75438 (+)
NCBI ID 836691992
GenBank LEKY01000074
Uniprot ID UPI00064A59A8
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 4.5e-64 87..288
OmpA PF00691.22 1.2e-09 359..458
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-267 MSEDNRRDDRASRVDATVLRPQGSSGSGSGADDATRIMAPRATGADAGATVISGGAYAAAGQGGARSAPRPALRAELGDFMSGVANPLVRAANPLLLLTVQLRHSVAPPADVARLREQAVAQVHSFESYAQGAGINTQTIMAARYVLCTMLDESVNNAPWGELSGWAQKTLLVTFHGETYGGAKFFQILDRLSVDFSRHLDLIELMYICLALGFGGRYLVEPGGLGRLADIQDDLYRRIRGLREAPAAELAPHWRGVEDRRNPVMRH
Transmembrane helix 268-290 VPLWVAMAAAAVILLASFLYFFT
Outside 291-468 RLNGLVEPVSAQLAAIGLQDVPPPQANAAPKPAGKSLKSLLAPLEQAGALTVDEQPDGRATVRLAAGLLFPSGGADIAPAEIPLLRQVAAALNQVKGRVIVVGHTDDQPIRSLKFKDNFALSTARAQNTLAILAQGLDDPRRLEANGAGSSQPIATPPSLPENRARNRRVEILYIPEN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.5841 - -


  Protein sequence: 469 a.a.    

>T6CP016247 LEKY01000074:74032-75438 [Burkholderia gladioli] [TssL]
MSEDNRRDDRASRVDATVLRPQGSSGSGSGADDATRIMAPRATGADAGATVISGGAYAAAGQGGARSAPRPALRAELGDF
MSGVANPLVRAANPLLLLTVQLRHSVAPPADVARLREQAVAQVHSFESYAQGAGINTQTIMAARYVLCTMLDESVNNAPW
GELSGWAQKTLLVTFHGETYGGAKFFQILDRLSVDFSRHLDLIELMYICLALGFGGRYLVEPGGLGRLADIQDDLYRRIR
GLREAPAAELAPHWRGVEDRRNPVMRHVPLWVAMAAAAVILLASFLYFFTRLNGLVEPVSAQLAAIGLQDVPPPQANAAP
KPAGKSLKSLLAPLEQAGALTVDEQPDGRATVRLAAGLLFPSGGADIAPAEIPLLRQVAAALNQVKGRVIVVGHTDDQPI
RSLKFKDNFALSTARAQNTLAILAQGLDDPRRLEANGAGSSQPIATPPSLPENRARNRRVEILYIPEN*

  Nucleotide sequence: 1407 bp    

>T6CP016247 LEKY01000074:74032-75438 [Burkholderia gladioli] [TssL]
ATGAGCGAGGACAACCGCAGGGACGATCGCGCGTCGCGCGTCGATGCCACCGTGCTGCGCCCGCAAGGCAGCAGCGGCTC
GGGTTCCGGCGCCGACGACGCGACCCGCATCATGGCGCCGCGCGCGACCGGCGCCGATGCCGGCGCGACGGTGATCTCTG
GCGGCGCTTATGCGGCGGCCGGGCAGGGCGGCGCGCGTTCCGCCCCGCGCCCCGCGCTGCGCGCCGAGCTCGGCGACTTC
ATGAGCGGCGTGGCCAATCCGCTGGTGCGCGCGGCCAATCCGCTGCTGCTGCTGACCGTGCAGCTTCGCCACAGCGTCGC
GCCGCCGGCCGACGTGGCGCGCCTGCGCGAGCAGGCGGTGGCCCAGGTGCACAGCTTCGAGAGCTACGCGCAGGGCGCCG
GCATCAACACCCAGACCATCATGGCCGCGCGCTACGTGCTCTGCACCATGCTCGACGAGTCCGTCAACAACGCGCCCTGG
GGCGAGCTGAGCGGCTGGGCGCAGAAGACCCTGCTGGTCACCTTCCACGGCGAGACCTACGGCGGCGCGAAGTTCTTCCA
GATCCTCGATCGCCTGAGCGTGGACTTCTCGCGCCATCTCGACCTGATCGAGCTGATGTACATCTGCCTGGCGCTCGGCT
TCGGCGGGCGCTACCTGGTCGAGCCGGGCGGCCTGGGGCGCCTGGCCGACATCCAGGACGACCTGTACCGCCGTATCCGC
GGCCTGCGCGAGGCGCCGGCCGCCGAGCTCGCGCCGCACTGGCGCGGCGTGGAGGATCGCCGCAACCCGGTGATGCGCCA
CGTGCCGCTGTGGGTGGCGATGGCCGCCGCCGCGGTGATCCTGCTGGCCAGCTTCCTCTACTTCTTCACGCGCCTGAACG
GCCTGGTGGAGCCGGTCAGCGCGCAGCTTGCCGCGATCGGCCTGCAGGACGTGCCGCCGCCGCAGGCCAACGCCGCGCCC
AAGCCGGCCGGCAAGAGCCTGAAGTCGCTGCTCGCGCCGCTCGAGCAGGCCGGCGCGCTGACCGTCGACGAGCAGCCCGA
CGGCCGCGCCACGGTGCGCCTGGCCGCGGGCCTGCTGTTCCCCTCGGGCGGCGCCGACATCGCGCCGGCCGAGATACCGC
TGCTGCGCCAGGTGGCGGCCGCGCTCAACCAGGTGAAGGGCCGCGTGATCGTGGTCGGCCATACCGACGACCAGCCGATC
CGCTCGCTGAAGTTCAAGGACAACTTCGCGCTGTCCACGGCGCGCGCCCAGAACACGCTGGCGATCCTGGCCCAGGGCCT
GGACGATCCGCGCCGCCTGGAAGCGAACGGCGCCGGCTCCTCGCAGCCGATCGCCACGCCGCCGAGCCTGCCGGAGAACC
GGGCACGCAATCGCCGCGTGGAAATCCTCTACATCCCGGAGAACTGA