Detailed information of component protein
Summary
| Component ID | T6CP016239 |
| Component protein type | TagJ |
| T6SS ID (Type) | T6SS01301  (Type i3) |
| Strain | Burkholderia gladioli strain NGJ1 |
| Replicon | chromosome |
| Sequence | Protein sequence (274 a.a.); Nucleotide sequence (822 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | ACI79_RS29620 |
| Coordinate (Strand) | 57922..58743 (+) |
| NCBI ID | 836691984 |
| GenBank | LEKY01000074 |
| Uniprot ID | UPI000649958F |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| ImpE | PF07024.15 | 1.2e-38 | 148..269 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-273 | VNPSLADTSPADAAALLAHGRLDEALSALSAEVRGRPADASLRVFLFQLLALVGEWDRAANQLKMAAELDERNTLMSTAYEFALRGEHERAAVLAGGKCPGIAGEPATWHAPLIRALHALTEKRIDETLAQRALAFDTAETVAGSIDGEPFEWIADADPRFGPCLELILKSGYVWAPFSQLRSLEFEAPSDLRDKVWAPVKLSWTSGAESVGFVPCRYAGSERSGIADLALAAGTSWVEHGEDCFTGRGQRMLVTDRAEYPLLDVRSITFGAR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9848 | - | - |
Protein sequence: 274 a.a.
>T6CP016239 LEKY01000074:57922-58743 [Burkholderia gladioli] [TagJ]
VNPSLADTSPADAAALLAHGRLDEALSALSAEVRGRPADASLRVFLFQLLALVGEWDRAANQLKMAAELDERNTLMSTAY
EFALRGEHERAAVLAGGKCPGIAGEPATWHAPLIRALHALTEKRIDETLAQRALAFDTAETVAGSIDGEPFEWIADADPR
FGPCLELILKSGYVWAPFSQLRSLEFEAPSDLRDKVWAPVKLSWTSGAESVGFVPCRYAGSERSGIADLALAAGTSWVEH
GEDCFTGRGQRMLVTDRAEYPLLDVRSITFGAR*
VNPSLADTSPADAAALLAHGRLDEALSALSAEVRGRPADASLRVFLFQLLALVGEWDRAANQLKMAAELDERNTLMSTAY
EFALRGEHERAAVLAGGKCPGIAGEPATWHAPLIRALHALTEKRIDETLAQRALAFDTAETVAGSIDGEPFEWIADADPR
FGPCLELILKSGYVWAPFSQLRSLEFEAPSDLRDKVWAPVKLSWTSGAESVGFVPCRYAGSERSGIADLALAAGTSWVEH
GEDCFTGRGQRMLVTDRAEYPLLDVRSITFGAR*
Nucleotide sequence: 822 bp
>T6CP016239 LEKY01000074:57922-58743 [Burkholderia gladioli] [TagJ]
GTGAACCCAAGCCTTGCCGACACCAGCCCGGCCGACGCCGCGGCGCTGCTTGCCCATGGACGCCTGGACGAGGCCCTGAG
CGCCCTGTCCGCCGAGGTCCGCGGCCGACCCGCCGATGCCTCGCTGCGCGTGTTCCTGTTCCAGTTGCTCGCGCTGGTGG
GCGAATGGGATCGCGCGGCCAACCAGTTGAAGATGGCGGCCGAACTCGACGAGCGGAACACGCTGATGTCGACCGCCTAT
GAATTCGCGCTGCGCGGCGAGCACGAGCGCGCGGCCGTGCTGGCCGGCGGCAAGTGCCCCGGCATCGCCGGCGAGCCGGC
CACCTGGCACGCGCCGCTGATCCGCGCGCTGCACGCGCTGACCGAGAAGCGCATCGACGAGACGCTGGCGCAGCGCGCGC
TCGCCTTCGACACGGCCGAGACCGTGGCCGGCAGCATCGACGGCGAGCCCTTCGAGTGGATCGCCGACGCCGATCCGCGC
TTCGGCCCCTGCCTGGAGCTGATCCTGAAGAGCGGCTACGTGTGGGCGCCGTTCTCGCAGTTGCGCTCGCTCGAGTTCGA
GGCACCCAGCGACCTGCGCGACAAGGTCTGGGCACCGGTCAAGCTGAGCTGGACGAGCGGCGCCGAATCGGTGGGCTTCG
TGCCCTGCCGCTACGCCGGCAGCGAGCGCTCCGGCATCGCCGACCTGGCGCTGGCCGCCGGCACCTCCTGGGTCGAGCAC
GGCGAGGACTGCTTCACCGGCCGCGGCCAGCGCATGCTGGTGACCGATCGCGCCGAGTACCCGCTGCTCGACGTGCGCTC
GATCACCTTCGGCGCGCGCTGA
GTGAACCCAAGCCTTGCCGACACCAGCCCGGCCGACGCCGCGGCGCTGCTTGCCCATGGACGCCTGGACGAGGCCCTGAG
CGCCCTGTCCGCCGAGGTCCGCGGCCGACCCGCCGATGCCTCGCTGCGCGTGTTCCTGTTCCAGTTGCTCGCGCTGGTGG
GCGAATGGGATCGCGCGGCCAACCAGTTGAAGATGGCGGCCGAACTCGACGAGCGGAACACGCTGATGTCGACCGCCTAT
GAATTCGCGCTGCGCGGCGAGCACGAGCGCGCGGCCGTGCTGGCCGGCGGCAAGTGCCCCGGCATCGCCGGCGAGCCGGC
CACCTGGCACGCGCCGCTGATCCGCGCGCTGCACGCGCTGACCGAGAAGCGCATCGACGAGACGCTGGCGCAGCGCGCGC
TCGCCTTCGACACGGCCGAGACCGTGGCCGGCAGCATCGACGGCGAGCCCTTCGAGTGGATCGCCGACGCCGATCCGCGC
TTCGGCCCCTGCCTGGAGCTGATCCTGAAGAGCGGCTACGTGTGGGCGCCGTTCTCGCAGTTGCGCTCGCTCGAGTTCGA
GGCACCCAGCGACCTGCGCGACAAGGTCTGGGCACCGGTCAAGCTGAGCTGGACGAGCGGCGCCGAATCGGTGGGCTTCG
TGCCCTGCCGCTACGCCGGCAGCGAGCGCTCCGGCATCGCCGACCTGGCGCTGGCCGCCGGCACCTCCTGGGTCGAGCAC
GGCGAGGACTGCTTCACCGGCCGCGGCCAGCGCATGCTGGTGACCGATCGCGCCGAGTACCCGCTGCTCGACGTGCGCTC
GATCACCTTCGGCGCGCGCTGA