Detailed information of component protein
Summary
| Component ID | T6CP016236 |
| Component protein type | TssB |
| T6SS ID (Type) | T6SS01301  (Type i3) |
| Strain | Burkholderia gladioli strain NGJ1 |
| Replicon | chromosome |
| Sequence | Protein sequence (174 a.a.); Nucleotide sequence (522 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | ACI79_RS29605 |
| Coordinate (Strand) | 55199..55720 (+) |
| NCBI ID | WP_013689196.1 |
| GenBank | LEKY01000074 |
| Uniprot ID | A0A560VM92_9BURK, A0A095W979_BURGA, F2LJJ7_BURGS |
| KEGG ID | bgd:bgla_2g03880, bgo:BM43_7143 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_VipA | PF05591.14 | 1.5e-56 | 12..162 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-173 | MATRQSSQKFIARNRAPRVQIEYDVELYGAQKKVQIPFVMGVMSDLSGANAGELPPIEERKALEIDVDNFDSRMQSMKPRVNFTVPNTLTGEGNLEVDISFESMDDFSPAAVARKVDSLARLLEARTQLANLGTYLDGKVGAEKLIAQALKDPALLQSLSSAPKAADTEPGQE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9856 | - | - |
Protein sequence: 174 a.a.
>T6CP016236 LEKY01000074:55199-55720 [Burkholderia gladioli] [TssB]
MATRQSSQKFIARNRAPRVQIEYDVELYGAQKKVQIPFVMGVMSDLSGANAGELPPIEERKALEIDVDNFDSRMQSMKPR
VNFTVPNTLTGEGNLEVDISFESMDDFSPAAVARKVDSLARLLEARTQLANLGTYLDGKVGAEKLIAQALKDPALLQSLS
SAPKAADTEPGQE*
MATRQSSQKFIARNRAPRVQIEYDVELYGAQKKVQIPFVMGVMSDLSGANAGELPPIEERKALEIDVDNFDSRMQSMKPR
VNFTVPNTLTGEGNLEVDISFESMDDFSPAAVARKVDSLARLLEARTQLANLGTYLDGKVGAEKLIAQALKDPALLQSLS
SAPKAADTEPGQE*
Nucleotide sequence: 522 bp
>T6CP016236 LEKY01000074:55199-55720 [Burkholderia gladioli] [TssB]
ATGGCAACGAGGCAGAGCAGTCAGAAATTCATTGCGCGCAATCGGGCGCCGCGCGTCCAGATCGAGTACGACGTCGAGTT
GTACGGCGCGCAGAAGAAAGTGCAGATCCCGTTCGTGATGGGCGTGATGTCGGACCTGTCCGGCGCCAATGCCGGCGAGC
TGCCGCCGATCGAGGAGCGCAAGGCGCTCGAGATCGACGTCGACAACTTCGACAGCCGCATGCAGTCGATGAAGCCGCGC
GTCAACTTCACGGTGCCGAACACGCTGACCGGCGAAGGCAACCTCGAGGTCGACATCAGCTTCGAGAGCATGGACGACTT
CTCGCCGGCCGCGGTGGCGCGCAAGGTGGATTCGCTCGCCAGGCTGCTCGAGGCGCGCACCCAGCTGGCCAATCTCGGCA
CCTATCTCGACGGCAAGGTCGGCGCGGAGAAGCTGATCGCGCAGGCGCTGAAGGACCCGGCGCTGCTGCAGTCGCTGAGC
TCGGCGCCCAAGGCCGCCGACACCGAACCCGGACAGGAGTGA
ATGGCAACGAGGCAGAGCAGTCAGAAATTCATTGCGCGCAATCGGGCGCCGCGCGTCCAGATCGAGTACGACGTCGAGTT
GTACGGCGCGCAGAAGAAAGTGCAGATCCCGTTCGTGATGGGCGTGATGTCGGACCTGTCCGGCGCCAATGCCGGCGAGC
TGCCGCCGATCGAGGAGCGCAAGGCGCTCGAGATCGACGTCGACAACTTCGACAGCCGCATGCAGTCGATGAAGCCGCGC
GTCAACTTCACGGTGCCGAACACGCTGACCGGCGAAGGCAACCTCGAGGTCGACATCAGCTTCGAGAGCATGGACGACTT
CTCGCCGGCCGCGGTGGCGCGCAAGGTGGATTCGCTCGCCAGGCTGCTCGAGGCGCGCACCCAGCTGGCCAATCTCGGCA
CCTATCTCGACGGCAAGGTCGGCGCGGAGAAGCTGATCGCGCAGGCGCTGAAGGACCCGGCGCTGCTGCAGTCGCTGAGC
TCGGCGCCCAAGGCCGCCGACACCGAACCCGGACAGGAGTGA