Detailed information of component protein


Summary

Component ID T6CP016235
Component protein type TssM
T6SS ID (Type) T6SS01300 experimental (Type i4b)
Strain Burkholderia gladioli strain NGJ1
Replicon chromosome
Sequence Protein sequence (1292 a.a.); Nucleotide sequence (3876 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) ACI79_RS13980
Coordinate (Strand) 107487..111362 (-)
NCBI ID 836689644
GenBank LEKY01000012
Uniprot ID UPI00064AF511
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 8.6e-96 497..849
IcmF-related_N PF14331.8 5.6e-80 193..448
IcmF_C PF06744.14 1.8e-21 1118..1220
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 MQRILNVLTHPR
Transmembrane helix 13-30 TLSILGFIALAAILFIVA
Outside 31-34 DALA
Transmembrane helix 35-57 ISFVYPLAILAVIVVLWLLVKLI
Inside 58-444 KRLRVRRANRKLGDMLEEQAQVDKAAAASVSPVEPAKAAELDTLRARLVDTVKTIKTSKIGQVSGGSALYELPWYIVIGNPAAGKSSAVINSGLQFPFADKNSAVIHGIGGTRNCDWFFTTEGILLDTAGRYSVHEEDRSEWIGFLGLLKKFRPKAPINGIIVTASIAELTGSRPEFAINLAKNLRQRVQELTEKLEVFAPVYVMFTKADLITGFTEFFSSSDRQEYDRVWGATLPYEPDEKRDVVALFDERFEELYDGLKEISVAQLSISRGNILSPGQLSFPLEFASIKPTLRAFLATLFENNPFQYKPIFRGFYFTSALQEGETNSAAAQRIASRFALDGSALPKPHSAFSKNGFFLRDLFSKVIFEDRKTVRQFASPAKTRMR
Transmembrane helix 445-467 YATFFAFVAVLALALGGWTWSTI
Outside 468-1291 GNQQLVANVQADLDNIAKLEQNRNDLQSRLQAMDILEDRIEQLEQFRRDKPLSVSLGLYQGDRLEERLLTEYYNGVRQIMLAPVADNLAAFLKDVDAHPDQLAPMTRTPESGAVPVSDKSALQPQAQGGLYNDASPTNVEDAYNALKTYLMLSDKRHVEQAHLTDQIARFWRGWLETNRGNMPRDEMIRSAERMITFYLSRVQDNDWPMIDANLALVDQTRENLRHVVRGMPARQRVYEEIKARASTRYAPMTIARIVGEDNTGLVAGSYAIPGTFTRDAWFDYVQPAIRDAATKELQAKDWVLNTASQDDLTLEGSPEQIQKVLVGMYKTEYAQHWQKFMQGIAVQNFGSFGQAVDAMNKLGDPQDSPIRKVLETAYDQTSWDNPSLANVSIKKAQTGVVNWVKQWFSRKPGGQLAANIDINGNPVEVPMGPIGQEFIGLGRIVQTHDGGQSMLKGYMDTLSKVRTRFNVIKNQGDPGPGARQLMQQTLDGNGSELADSLKYVDEQMLTGLTDSQRKSLRPLLVRPLQQAFSVVIQPASVEVNKVWNAQVYQPFQASLANKYPFSASAKVEAASSEIAQVFGPDGSISKFVGTTLGPLAVRRGDTLTARTWGDMGLALTPEFTSGFATWVAPLAGGAATSAASSEPQTVFQILPQPSSGTTEYTIAIDGQQLRYRNTPPQWTNFVWPNPSGSPGAMLTATTFDGRTLTLVNEPGRYGLEKLINSAQRSRRPDGTFDLTWTQGNVNVAVTMRIISTSQPSGGGSGGGDAPQQQSLRGLHLPSSVANTSASGAVNATAQAPAGTSGVATAANGAAANASNAQGAQ


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9966 - -


  Protein sequence: 1292 a.a.    

>T6CP016235 LEKY01000012:c111362-107487 [Burkholderia gladioli] [TssM]
MQRILNVLTHPRTLSILGFIALAAILFIVADALAISFVYPLAILAVIVVLWLLVKLIKRLRVRRANRKLGDMLEEQAQVD
KAAAASVSPVEPAKAAELDTLRARLVDTVKTIKTSKIGQVSGGSALYELPWYIVIGNPAAGKSSAVINSGLQFPFADKNS
AVIHGIGGTRNCDWFFTTEGILLDTAGRYSVHEEDRSEWIGFLGLLKKFRPKAPINGIIVTASIAELTGSRPEFAINLAK
NLRQRVQELTEKLEVFAPVYVMFTKADLITGFTEFFSSSDRQEYDRVWGATLPYEPDEKRDVVALFDERFEELYDGLKEI
SVAQLSISRGNILSPGQLSFPLEFASIKPTLRAFLATLFENNPFQYKPIFRGFYFTSALQEGETNSAAAQRIASRFALDG
SALPKPHSAFSKNGFFLRDLFSKVIFEDRKTVRQFASPAKTRMRYATFFAFVAVLALALGGWTWSTIGNQQLVANVQADL
DNIAKLEQNRNDLQSRLQAMDILEDRIEQLEQFRRDKPLSVSLGLYQGDRLEERLLTEYYNGVRQIMLAPVADNLAAFLK
DVDAHPDQLAPMTRTPESGAVPVSDKSALQPQAQGGLYNDASPTNVEDAYNALKTYLMLSDKRHVEQAHLTDQIARFWRG
WLETNRGNMPRDEMIRSAERMITFYLSRVQDNDWPMIDANLALVDQTRENLRHVVRGMPARQRVYEEIKARASTRYAPMT
IARIVGEDNTGLVAGSYAIPGTFTRDAWFDYVQPAIRDAATKELQAKDWVLNTASQDDLTLEGSPEQIQKVLVGMYKTEY
AQHWQKFMQGIAVQNFGSFGQAVDAMNKLGDPQDSPIRKVLETAYDQTSWDNPSLANVSIKKAQTGVVNWVKQWFSRKPG
GQLAANIDINGNPVEVPMGPIGQEFIGLGRIVQTHDGGQSMLKGYMDTLSKVRTRFNVIKNQGDPGPGARQLMQQTLDGN
GSELADSLKYVDEQMLTGLTDSQRKSLRPLLVRPLQQAFSVVIQPASVEVNKVWNAQVYQPFQASLANKYPFSASAKVEA
ASSEIAQVFGPDGSISKFVGTTLGPLAVRRGDTLTARTWGDMGLALTPEFTSGFATWVAPLAGGAATSAASSEPQTVFQI
LPQPSSGTTEYTIAIDGQQLRYRNTPPQWTNFVWPNPSGSPGAMLTATTFDGRTLTLVNEPGRYGLEKLINSAQRSRRPD
GTFDLTWTQGNVNVAVTMRIISTSQPSGGGSGGGDAPQQQSLRGLHLPSSVANTSASGAVNATAQAPAGTSGVATAANGA
AANASNAQGAQ*

  Nucleotide sequence: 3876 bp    

>T6CP016235 LEKY01000012:c111362-107487 [Burkholderia gladioli] [TssM]
ATGCAACGCATCCTCAACGTGTTGACTCACCCGCGCACGCTCTCGATCCTCGGGTTCATCGCGCTCGCCGCGATCCTGTT
CATCGTGGCGGACGCCCTTGCGATCAGCTTCGTCTACCCGCTCGCGATCCTCGCGGTGATCGTGGTGCTGTGGCTGCTGG
TGAAGCTGATCAAGCGGCTGCGCGTGCGGCGCGCCAACCGCAAGCTCGGCGACATGCTCGAGGAGCAGGCCCAGGTCGAC
AAGGCGGCGGCCGCCTCGGTGAGCCCGGTCGAACCGGCCAAGGCCGCCGAGCTCGACACGCTGCGCGCGCGCCTGGTGGA
CACGGTCAAGACCATCAAGACCTCGAAGATCGGCCAGGTGTCCGGCGGCTCGGCGCTCTACGAGCTGCCCTGGTACATCG
TGATCGGCAACCCGGCGGCGGGCAAGAGCAGCGCGGTGATCAACTCGGGCCTGCAGTTCCCGTTCGCCGACAAGAACAGC
GCGGTGATCCACGGCATCGGCGGCACCCGCAACTGCGACTGGTTCTTCACCACCGAGGGCATCCTGCTCGACACCGCCGG
CCGCTACTCGGTGCACGAGGAAGACCGCAGCGAATGGATCGGCTTCCTCGGCCTGCTCAAGAAATTCCGTCCCAAGGCGC
CGATCAACGGCATCATCGTCACCGCCAGCATCGCCGAGCTGACCGGCAGCCGTCCCGAGTTCGCCATCAACCTGGCCAAG
AACCTGCGCCAGCGCGTGCAGGAGCTGACCGAGAAGCTGGAGGTGTTCGCGCCGGTCTACGTGATGTTCACCAAGGCCGA
CCTGATCACCGGCTTCACCGAGTTCTTCAGCAGCAGCGACCGCCAGGAATACGACCGCGTGTGGGGCGCCACCCTGCCCT
ACGAGCCCGACGAGAAGCGCGACGTGGTGGCGCTGTTCGACGAGCGCTTCGAGGAACTCTACGACGGCCTCAAGGAAATC
AGCGTCGCGCAGCTGTCGATCAGCCGCGGCAACATCCTCTCGCCGGGCCAGCTGAGCTTCCCGCTCGAATTCGCCTCGAT
CAAGCCCACCCTGCGCGCCTTCCTGGCCACCCTGTTCGAGAACAACCCGTTCCAGTACAAGCCGATCTTCCGCGGCTTCT
ACTTCACCAGCGCGCTGCAGGAAGGCGAAACCAATTCCGCCGCCGCGCAGCGCATCGCCTCGCGCTTCGCGCTGGACGGC
AGCGCCCTGCCCAAGCCGCACAGCGCCTTCTCGAAGAACGGCTTCTTCCTGCGCGACCTGTTCTCGAAGGTGATCTTCGA
GGACCGCAAGACGGTGCGCCAGTTCGCCAGCCCCGCCAAGACGCGGATGCGCTACGCCACCTTCTTCGCCTTCGTGGCGG
TGCTGGCGCTGGCGCTGGGCGGCTGGACCTGGTCAACGATCGGCAACCAGCAGCTGGTGGCCAACGTGCAGGCCGACCTC
GACAACATCGCCAAGCTCGAGCAGAACCGCAACGATCTGCAATCGCGCCTGCAGGCGATGGACATCCTCGAGGACCGCAT
CGAGCAGCTCGAGCAGTTCCGCCGCGACAAGCCGCTCTCGGTCTCGCTGGGCCTCTACCAGGGCGACCGCCTGGAAGAGC
GCCTGCTCACCGAGTACTACAACGGCGTGCGCCAGATCATGCTGGCGCCGGTGGCCGACAATCTCGCCGCGTTCCTGAAG
GACGTCGACGCGCATCCGGACCAGCTCGCGCCGATGACGCGCACGCCAGAATCGGGCGCGGTGCCGGTCTCCGACAAATC
GGCGCTGCAGCCGCAGGCGCAGGGCGGGCTCTACAACGACGCCTCGCCGACCAATGTCGAGGACGCCTACAACGCGCTGA
AGACCTACCTGATGCTGTCGGACAAGCGCCACGTCGAGCAGGCGCACCTGACCGACCAGATCGCGCGCTTCTGGCGCGGC
TGGCTGGAAACCAATCGCGGGAACATGCCGCGCGACGAGATGATCCGCAGCGCCGAGCGCATGATCACCTTCTACCTGTC
GCGCGTGCAGGACAACGACTGGCCGATGATCGACGCCAACCTGGCCCTGGTCGACCAGACCCGCGAGAACCTGCGCCACG
TGGTGCGCGGCATGCCGGCCCGCCAGCGCGTCTACGAGGAAATCAAGGCGCGCGCCTCGACGCGTTATGCCCCGATGACG
ATCGCGCGCATCGTCGGCGAGGACAACACCGGCCTGGTGGCCGGCAGCTACGCGATCCCCGGCACCTTCACGCGCGACGC
CTGGTTCGACTACGTGCAGCCGGCGATCCGCGACGCGGCCACCAAGGAGCTGCAGGCCAAGGACTGGGTGCTCAACACCG
CCTCACAGGACGACCTGACGCTGGAAGGCAGCCCCGAGCAGATCCAGAAGGTGCTGGTCGGCATGTACAAGACCGAGTAC
GCCCAGCACTGGCAGAAGTTCATGCAGGGCATCGCGGTGCAGAACTTCGGCAGCTTCGGCCAGGCCGTGGACGCCATGAA
CAAGCTCGGCGACCCGCAGGACTCGCCGATCCGCAAGGTGCTCGAGACCGCCTACGACCAGACTTCCTGGGATAACCCGT
CGCTGGCCAACGTGTCGATCAAGAAGGCGCAGACCGGCGTGGTGAACTGGGTCAAGCAGTGGTTCTCGCGCAAGCCTGGC
GGGCAGCTGGCCGCCAACATCGACATCAACGGCAACCCGGTCGAGGTGCCGATGGGCCCGATCGGCCAGGAGTTCATCGG
CCTGGGGCGGATCGTGCAGACCCACGACGGCGGCCAGTCGATGCTCAAGGGCTACATGGACACGCTCTCCAAGGTGCGCA
CCCGCTTCAACGTGATCAAGAACCAGGGCGACCCCGGCCCCGGCGCGCGCCAGCTGATGCAGCAGACGCTCGACGGCAAC
GGCTCGGAACTGGCCGATTCGCTGAAGTACGTCGACGAGCAGATGCTGACCGGCCTGACCGACTCGCAACGCAAGTCGCT
GCGCCCGCTGCTGGTGCGCCCGCTGCAGCAGGCCTTCTCGGTGGTGATCCAGCCGGCCTCGGTGGAAGTCAACAAGGTGT
GGAACGCGCAGGTCTACCAGCCGTTCCAGGCCTCGCTGGCGAACAAGTACCCGTTCTCCGCCAGTGCCAAGGTGGAGGCC
GCCTCGAGCGAGATCGCACAGGTGTTCGGCCCGGACGGCTCGATCTCGAAGTTCGTCGGCACCACGCTCGGGCCGCTCGC
GGTGCGCCGCGGCGACACGCTCACGGCACGCACCTGGGGCGACATGGGTCTGGCGCTGACGCCGGAATTCACCAGCGGCT
TCGCGACCTGGGTCGCGCCGCTGGCGGGCGGCGCCGCCACCAGCGCGGCCTCCTCCGAGCCGCAGACGGTGTTCCAGATC
CTGCCGCAGCCGAGCAGCGGGACCACCGAGTACACCATCGCGATCGACGGCCAGCAGCTGCGCTACCGCAACACGCCGCC
GCAGTGGACCAACTTCGTCTGGCCGAACCCGAGCGGCTCGCCGGGCGCGATGCTGACGGCCACCACCTTCGACGGCCGCA
CGCTGACCCTGGTCAACGAGCCGGGCCGTTACGGGCTGGAGAAGCTGATCAACTCGGCGCAGCGCTCGCGCCGCCCGGAC
GGCACCTTCGACCTGACCTGGACGCAGGGCAACGTCAACGTGGCGGTGACGATGCGCATCATCAGCACCTCGCAGCCCTC
GGGCGGCGGCAGCGGTGGCGGCGACGCGCCGCAGCAGCAAAGCCTGCGCGGCCTGCACCTGCCCTCGTCGGTGGCGAACA
CCAGCGCGAGCGGCGCCGTCAACGCGACGGCCCAGGCACCTGCCGGCACGTCCGGCGTCGCAACGGCCGCCAACGGCGCG
GCCGCCAACGCATCGAACGCACAGGGGGCGCAATGA