Detailed information of component protein
Summary
| Component ID | T6CP016228 |
| Component protein type | TssE |
| T6SS ID (Type) | T6SS01300  (Type i4b) |
| Strain | Burkholderia gladioli strain NGJ1 |
| Replicon | chromosome |
| Sequence | Protein sequence (161 a.a.); Nucleotide sequence (483 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | ACI79_RS13945 |
| Coordinate (Strand) | 97865..98347 (+) |
| NCBI ID | 503461805 |
| GenBank | LEKY01000012 |
| Uniprot ID | A0A095W7S9_BURGA, F2LI29_BURGS |
| KEGG ID | bgo:BM43_1800, bgd:bgla_1g04130 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| GPW_gp25 | PF04965.16 | 2.2e-21 | 32..132 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-160 | MKRFEPSFLDKLFDDEPHQPASPAMRQLSLDELKSTVARDVESILNTRIALTDEDLHALPECQQSVLTYGLNDFAGLSLASHYDRAFICKSIQQAIDRHEPRLRQVAVSFELNEQSTNSLNFAIRAMLVVHPAQEPVSFDAMLQPSTLQYSVTRGRAAKV |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9844 | - | - |
Protein sequence: 161 a.a.
>T6CP016228 LEKY01000012:97865-98347 [Burkholderia gladioli] [TssE]
MKRFEPSFLDKLFDDEPHQPASPAMRQLSLDELKSTVARDVESILNTRIALTDEDLHALPECQQSVLTYGLNDFAGLSLA
SHYDRAFICKSIQQAIDRHEPRLRQVAVSFELNEQSTNSLNFAIRAMLVVHPAQEPVSFDAMLQPSTLQYSVTRGRAAKV
*
MKRFEPSFLDKLFDDEPHQPASPAMRQLSLDELKSTVARDVESILNTRIALTDEDLHALPECQQSVLTYGLNDFAGLSLA
SHYDRAFICKSIQQAIDRHEPRLRQVAVSFELNEQSTNSLNFAIRAMLVVHPAQEPVSFDAMLQPSTLQYSVTRGRAAKV
*
Nucleotide sequence: 483 bp
>T6CP016228 LEKY01000012:97865-98347 [Burkholderia gladioli] [TssE]
ATGAAACGTTTCGAGCCGAGTTTCCTCGACAAGCTGTTCGACGACGAACCGCATCAGCCGGCCTCGCCCGCGATGCGGCA
ATTGTCGCTGGACGAGCTGAAAAGCACGGTCGCCCGCGATGTCGAGTCGATCCTGAACACGCGCATCGCCCTGACCGACG
AAGATCTCCATGCGCTGCCGGAATGCCAGCAATCGGTGCTGACCTACGGCCTCAACGATTTCGCGGGCCTGAGCCTCGCG
AGCCACTACGACCGTGCCTTCATCTGCAAGTCGATCCAGCAGGCGATCGACCGGCACGAGCCGCGCCTGCGGCAGGTGGC
GGTATCGTTCGAACTGAACGAGCAATCCACCAATTCGCTGAATTTCGCGATTCGCGCGATGCTGGTCGTGCATCCGGCGC
AGGAGCCCGTCAGTTTCGACGCGATGCTGCAGCCGTCCACATTGCAGTACTCGGTGACGCGCGGACGCGCCGCCAAGGTT
TGA
ATGAAACGTTTCGAGCCGAGTTTCCTCGACAAGCTGTTCGACGACGAACCGCATCAGCCGGCCTCGCCCGCGATGCGGCA
ATTGTCGCTGGACGAGCTGAAAAGCACGGTCGCCCGCGATGTCGAGTCGATCCTGAACACGCGCATCGCCCTGACCGACG
AAGATCTCCATGCGCTGCCGGAATGCCAGCAATCGGTGCTGACCTACGGCCTCAACGATTTCGCGGGCCTGAGCCTCGCG
AGCCACTACGACCGTGCCTTCATCTGCAAGTCGATCCAGCAGGCGATCGACCGGCACGAGCCGCGCCTGCGGCAGGTGGC
GGTATCGTTCGAACTGAACGAGCAATCCACCAATTCGCTGAATTTCGCGATTCGCGCGATGCTGGTCGTGCATCCGGCGC
AGGAGCCCGTCAGTTTCGACGCGATGCTGCAGCCGTCCACATTGCAGTACTCGGTGACGCGCGGACGCGCCGCCAAGGTT
TGA