Detailed information of component protein
Summary
| Component ID | T6CP016225 |
| Component protein type | TssB |
| T6SS ID (Type) | T6SS01300  (Type i4b) |
| Strain | Burkholderia gladioli strain NGJ1 |
| Replicon | chromosome |
| Sequence | Protein sequence (173 a.a.); Nucleotide sequence (519 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | ACI79_RS13930 |
| Coordinate (Strand) | 95172..95690 (+) |
| NCBI ID | WP_013696463.1 |
| GenBank | LEKY01000012 |
| Uniprot ID | A0A560UTC8_9BURK, A0A095XG85_BURGA, F2LI26_BURGS |
| KEGG ID | bgo:BM43_1797, bgd:bgla_1g04100 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_VipA | PF05591.14 | 3.1e-60 | 12..163 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-172 | MAKKESIQKRLQKVRPPRVQLTYEVERGDAIEVKELPFVVGVVGDLAGQSEVEMPKLRDRKFVNIDRDNFDDVMKAIEPRAAYQVENKLSEEGGKFAVDLKFRSLDDFDPDQVVNQIEPLRRLIEARSKLADLRNKLAGNDKLEDLLSEVLSNTQQLQALAKQSGGGDKEGE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9963 | - | - |
Protein sequence: 173 a.a.
>T6CP016225 LEKY01000012:95172-95690 [Burkholderia gladioli] [TssB]
MAKKESIQKRLQKVRPPRVQLTYEVERGDAIEVKELPFVVGVVGDLAGQSEVEMPKLRDRKFVNIDRDNFDDVMKAIEPR
AAYQVENKLSEEGGKFAVDLKFRSLDDFDPDQVVNQIEPLRRLIEARSKLADLRNKLAGNDKLEDLLSEVLSNTQQLQAL
AKQSGGGDKEGE*
MAKKESIQKRLQKVRPPRVQLTYEVERGDAIEVKELPFVVGVVGDLAGQSEVEMPKLRDRKFVNIDRDNFDDVMKAIEPR
AAYQVENKLSEEGGKFAVDLKFRSLDDFDPDQVVNQIEPLRRLIEARSKLADLRNKLAGNDKLEDLLSEVLSNTQQLQAL
AKQSGGGDKEGE*
Nucleotide sequence: 519 bp
>T6CP016225 LEKY01000012:95172-95690 [Burkholderia gladioli] [TssB]
ATGGCGAAAAAAGAAAGTATCCAGAAGCGCCTGCAAAAGGTACGGCCGCCCCGTGTGCAGCTGACCTACGAAGTCGAGCG
CGGCGACGCGATCGAGGTGAAGGAACTGCCGTTCGTGGTCGGCGTGGTCGGCGACCTGGCCGGTCAGTCCGAGGTCGAGA
TGCCGAAGCTGCGCGACCGCAAGTTCGTCAACATCGATCGCGACAACTTCGACGACGTGATGAAGGCGATCGAGCCTCGC
GCGGCCTACCAGGTCGAGAACAAACTCAGCGAGGAAGGCGGCAAGTTCGCCGTCGACCTCAAGTTCCGTTCGCTCGACGA
TTTCGATCCGGACCAGGTGGTCAACCAGATCGAACCGCTGCGCCGCCTGATCGAGGCGCGCTCGAAGCTGGCCGACCTGC
GCAACAAGCTGGCCGGCAACGACAAGCTCGAGGACCTGCTGAGCGAAGTGCTCAGCAACACGCAGCAGTTGCAGGCGCTC
GCGAAGCAGTCGGGCGGCGGCGACAAAGAGGGCGAATAA
ATGGCGAAAAAAGAAAGTATCCAGAAGCGCCTGCAAAAGGTACGGCCGCCCCGTGTGCAGCTGACCTACGAAGTCGAGCG
CGGCGACGCGATCGAGGTGAAGGAACTGCCGTTCGTGGTCGGCGTGGTCGGCGACCTGGCCGGTCAGTCCGAGGTCGAGA
TGCCGAAGCTGCGCGACCGCAAGTTCGTCAACATCGATCGCGACAACTTCGACGACGTGATGAAGGCGATCGAGCCTCGC
GCGGCCTACCAGGTCGAGAACAAACTCAGCGAGGAAGGCGGCAAGTTCGCCGTCGACCTCAAGTTCCGTTCGCTCGACGA
TTTCGATCCGGACCAGGTGGTCAACCAGATCGAACCGCTGCGCCGCCTGATCGAGGCGCGCTCGAAGCTGGCCGACCTGC
GCAACAAGCTGGCCGGCAACGACAAGCTCGAGGACCTGCTGAGCGAAGTGCTCAGCAACACGCAGCAGTTGCAGGCGCTC
GCGAAGCAGTCGGGCGGCGGCGACAAAGAGGGCGAATAA