Detailed information of component protein


Summary

Component ID T6CP016224
Component protein type TssJ
T6SS ID (Type) T6SS01300 experimental (Type i4b)
Strain Burkholderia gladioli strain NGJ1
Replicon chromosome
Sequence Protein sequence (206 a.a.); Nucleotide sequence (618 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) ACI79_RS13920
Coordinate (Strand) 93494..94111 (-)
NCBI ID 817530996
GenBank LEKY01000012
Uniprot ID A0A0M2Q8S2_BURGA
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS-SciN PF12790.9 2.4e-28 55..173
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-6 MTPSFP
Transmembrane helix 7-29 RFALPLVACALLAGCAAAVPLLG
Outside 30-205 SAGSTALQMVGLGKPEVPDAQKPPRNLGITLYAAPNLNAANDNKPLALVVRLYTLKDPTSFQQAPFDTFTDPAKEKAALGADLLGVREVTLIPGQRYNATEKVSREAQAFGIVALFRSPALQRWKLAFDPAKSEKSGIIIGLHNCAMTVTDGSVIAPQQGMPAQQLNMLSSVSCGN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.8995 pos: 20-21. LAG-CA. MTPSFPRFALPLVACALLAG


  Protein sequence: 206 a.a.    

>T6CP016224 LEKY01000012:c94111-93494 [Burkholderia gladioli] [TssJ]
MTPSFPRFALPLVACALLAGCAAAVPLLGSAGSTALQMVGLGKPEVPDAQKPPRNLGITLYAAPNLNAANDNKPLALVVR
LYTLKDPTSFQQAPFDTFTDPAKEKAALGADLLGVREVTLIPGQRYNATEKVSREAQAFGIVALFRSPALQRWKLAFDPA
KSEKSGIIIGLHNCAMTVTDGSVIAPQQGMPAQQLNMLSSVSCGN*

  Nucleotide sequence: 618 bp    

>T6CP016224 LEKY01000012:c94111-93494 [Burkholderia gladioli] [TssJ]
ATGACTCCGTCCTTTCCCCGCTTCGCGCTGCCGCTCGTGGCCTGCGCCTTACTGGCAGGCTGCGCGGCGGCCGTGCCGTT
GCTCGGTTCGGCCGGCAGCACGGCCCTGCAGATGGTCGGCCTCGGCAAGCCCGAGGTGCCCGATGCGCAGAAGCCGCCAC
GCAATTTAGGCATTACCCTCTACGCGGCGCCGAACCTCAACGCGGCCAATGACAATAAGCCGCTGGCGCTGGTGGTGCGG
CTCTACACGCTGAAGGACCCGACCTCGTTCCAGCAAGCGCCGTTCGACACCTTCACCGATCCGGCCAAGGAGAAGGCCGC
GCTCGGCGCCGACCTGCTCGGCGTGCGCGAGGTCACCCTGATCCCGGGGCAGCGCTACAACGCCACCGAGAAGGTCTCGC
GCGAGGCGCAGGCGTTCGGCATCGTCGCGCTGTTCCGCAGCCCCGCGCTGCAGCGCTGGAAGCTGGCTTTCGATCCGGCG
AAATCGGAGAAATCCGGCATAATCATCGGCCTGCACAACTGTGCGATGACCGTGACGGATGGCAGCGTGATTGCGCCGCA
GCAGGGTATGCCGGCCCAGCAGCTGAATATGCTCTCATCAGTCAGCTGCGGCAATTAA