Detailed information of component protein


Summary

Component ID T6CP016222
Component protein type TssL
T6SS ID (Type) T6SS01300 experimental (Type i4b)
Strain Burkholderia gladioli strain NGJ1
Replicon chromosome
Sequence Protein sequence (262 a.a.); Nucleotide sequence (786 bp)
Reference
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) ACI79_RS13910
Coordinate (Strand) 91265..92050 (-)
NCBI ID 503461798
GenBank LEKY01000012
Uniprot ID A0A095W8B3_BURGA, F2LI22_BURGS
KEGG ID bgd:bgla_1g04060, bgo:BM43_1793
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 2.5e-58 32..229

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-209 MSYAPSLFGGTTPPPAPHSASSTDASFQARSLLDLLYDGFFMMFLLKNGREPGEANEFRTRIQEFLADFERGAKKLNAGADDVYAAKFAFCAAIDEAVLSSQFKVRAEWERRPLQLVLFGEQLAGEKFFQYLEECRAHGVARLQSLEVFHMCLLLGFQGKYLLEGPEKLAYLTARIGDEIAQMKGKRAPFAPHWALPDQIAHRLKREVP
Transmembrane helix 210-232 LWAIGAVFALVGLLAFVGLNAYL
Inside 233-261 RDHTLKALAPYSQIIKVGPESANLTISLP



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9324 - -



  Protein sequence: 262 a.a.    

>T6CP016222 LEKY01000012:c92050-91265 [Burkholderia gladioli] [TssL]
MSYAPSLFGGTTPPPAPHSASSTDASFQARSLLDLLYDGFFMMFLLKNGREPGEANEFRTRIQEFLADFERGAKKLNAGA
DDVYAAKFAFCAAIDEAVLSSQFKVRAEWERRPLQLVLFGEQLAGEKFFQYLEECRAHGVARLQSLEVFHMCLLLGFQGK
YLLEGPEKLAYLTARIGDEIAQMKGKRAPFAPHWALPDQIAHRLKREVPLWAIGAVFALVGLLAFVGLNAYLRDHTLKAL
APYSQIIKVGPESANLTISLP*

  Nucleotide sequence: 786 bp    

>T6CP016222 LEKY01000012:c92050-91265 [Burkholderia gladioli] [TssL]
ATGAGCTACGCGCCCTCCCTGTTCGGCGGCACCACGCCGCCACCCGCCCCGCATTCGGCCTCGTCGACCGACGCCAGCTT
CCAGGCGCGCTCGCTGCTCGACCTGCTCTATGACGGCTTCTTCATGATGTTCCTGCTGAAGAACGGTCGCGAGCCCGGCG
AGGCGAACGAGTTCCGCACGCGGATCCAGGAGTTCCTGGCCGATTTCGAGCGCGGCGCGAAGAAGCTCAATGCCGGCGCC
GACGACGTCTACGCCGCCAAGTTCGCGTTCTGCGCGGCGATCGACGAGGCGGTGCTGTCCTCGCAGTTCAAGGTGCGTGC
CGAGTGGGAGCGCCGCCCGCTGCAGTTGGTGCTGTTCGGCGAGCAACTGGCCGGCGAGAAGTTCTTCCAGTACCTGGAGG
AATGCCGCGCGCACGGCGTGGCGCGCCTGCAGTCGCTGGAGGTGTTCCACATGTGCCTGCTGCTGGGCTTCCAGGGCAAG
TACCTGCTCGAGGGACCGGAGAAGCTCGCCTACCTGACCGCGCGGATCGGCGACGAGATCGCTCAGATGAAGGGCAAGCG
CGCGCCCTTCGCGCCGCATTGGGCGCTGCCCGACCAGATCGCGCACCGCCTCAAGCGCGAGGTGCCGTTGTGGGCAATCG
GCGCCGTGTTCGCGCTGGTCGGCCTGCTGGCCTTCGTCGGGCTCAACGCCTATCTGCGCGATCACACGCTGAAGGCGCTG
GCGCCCTATTCGCAGATCATCAAGGTCGGGCCGGAATCGGCCAACCTGACGATCTCGCTGCCCTGA