Detailed information of component protein
Summary
| Component ID | T6CP016222 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS01300  (Type i4b) |
| Strain | Burkholderia gladioli strain NGJ1 |
| Replicon | chromosome |
| Sequence | Protein sequence (262 a.a.); Nucleotide sequence (786 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | ACI79_RS13910 |
| Coordinate (Strand) | 91265..92050 (-) |
| NCBI ID | 503461798 |
| GenBank | LEKY01000012 |
| Uniprot ID | A0A095W8B3_BURGA, F2LI22_BURGS |
| KEGG ID | bgd:bgla_1g04060, bgo:BM43_1793 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 2.5e-58 | 32..229 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-209 | MSYAPSLFGGTTPPPAPHSASSTDASFQARSLLDLLYDGFFMMFLLKNGREPGEANEFRTRIQEFLADFERGAKKLNAGADDVYAAKFAFCAAIDEAVLSSQFKVRAEWERRPLQLVLFGEQLAGEKFFQYLEECRAHGVARLQSLEVFHMCLLLGFQGKYLLEGPEKLAYLTARIGDEIAQMKGKRAPFAPHWALPDQIAHRLKREVP |
| Transmembrane helix | 210-232 | LWAIGAVFALVGLLAFVGLNAYL |
| Inside | 233-261 | RDHTLKALAPYSQIIKVGPESANLTISLP |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9324 | - | - |
Protein sequence: 262 a.a.
>T6CP016222 LEKY01000012:c92050-91265 [Burkholderia gladioli] [TssL]
MSYAPSLFGGTTPPPAPHSASSTDASFQARSLLDLLYDGFFMMFLLKNGREPGEANEFRTRIQEFLADFERGAKKLNAGA
DDVYAAKFAFCAAIDEAVLSSQFKVRAEWERRPLQLVLFGEQLAGEKFFQYLEECRAHGVARLQSLEVFHMCLLLGFQGK
YLLEGPEKLAYLTARIGDEIAQMKGKRAPFAPHWALPDQIAHRLKREVPLWAIGAVFALVGLLAFVGLNAYLRDHTLKAL
APYSQIIKVGPESANLTISLP*
MSYAPSLFGGTTPPPAPHSASSTDASFQARSLLDLLYDGFFMMFLLKNGREPGEANEFRTRIQEFLADFERGAKKLNAGA
DDVYAAKFAFCAAIDEAVLSSQFKVRAEWERRPLQLVLFGEQLAGEKFFQYLEECRAHGVARLQSLEVFHMCLLLGFQGK
YLLEGPEKLAYLTARIGDEIAQMKGKRAPFAPHWALPDQIAHRLKREVPLWAIGAVFALVGLLAFVGLNAYLRDHTLKAL
APYSQIIKVGPESANLTISLP*
Nucleotide sequence: 786 bp
>T6CP016222 LEKY01000012:c92050-91265 [Burkholderia gladioli] [TssL]
ATGAGCTACGCGCCCTCCCTGTTCGGCGGCACCACGCCGCCACCCGCCCCGCATTCGGCCTCGTCGACCGACGCCAGCTT
CCAGGCGCGCTCGCTGCTCGACCTGCTCTATGACGGCTTCTTCATGATGTTCCTGCTGAAGAACGGTCGCGAGCCCGGCG
AGGCGAACGAGTTCCGCACGCGGATCCAGGAGTTCCTGGCCGATTTCGAGCGCGGCGCGAAGAAGCTCAATGCCGGCGCC
GACGACGTCTACGCCGCCAAGTTCGCGTTCTGCGCGGCGATCGACGAGGCGGTGCTGTCCTCGCAGTTCAAGGTGCGTGC
CGAGTGGGAGCGCCGCCCGCTGCAGTTGGTGCTGTTCGGCGAGCAACTGGCCGGCGAGAAGTTCTTCCAGTACCTGGAGG
AATGCCGCGCGCACGGCGTGGCGCGCCTGCAGTCGCTGGAGGTGTTCCACATGTGCCTGCTGCTGGGCTTCCAGGGCAAG
TACCTGCTCGAGGGACCGGAGAAGCTCGCCTACCTGACCGCGCGGATCGGCGACGAGATCGCTCAGATGAAGGGCAAGCG
CGCGCCCTTCGCGCCGCATTGGGCGCTGCCCGACCAGATCGCGCACCGCCTCAAGCGCGAGGTGCCGTTGTGGGCAATCG
GCGCCGTGTTCGCGCTGGTCGGCCTGCTGGCCTTCGTCGGGCTCAACGCCTATCTGCGCGATCACACGCTGAAGGCGCTG
GCGCCCTATTCGCAGATCATCAAGGTCGGGCCGGAATCGGCCAACCTGACGATCTCGCTGCCCTGA
ATGAGCTACGCGCCCTCCCTGTTCGGCGGCACCACGCCGCCACCCGCCCCGCATTCGGCCTCGTCGACCGACGCCAGCTT
CCAGGCGCGCTCGCTGCTCGACCTGCTCTATGACGGCTTCTTCATGATGTTCCTGCTGAAGAACGGTCGCGAGCCCGGCG
AGGCGAACGAGTTCCGCACGCGGATCCAGGAGTTCCTGGCCGATTTCGAGCGCGGCGCGAAGAAGCTCAATGCCGGCGCC
GACGACGTCTACGCCGCCAAGTTCGCGTTCTGCGCGGCGATCGACGAGGCGGTGCTGTCCTCGCAGTTCAAGGTGCGTGC
CGAGTGGGAGCGCCGCCCGCTGCAGTTGGTGCTGTTCGGCGAGCAACTGGCCGGCGAGAAGTTCTTCCAGTACCTGGAGG
AATGCCGCGCGCACGGCGTGGCGCGCCTGCAGTCGCTGGAGGTGTTCCACATGTGCCTGCTGCTGGGCTTCCAGGGCAAG
TACCTGCTCGAGGGACCGGAGAAGCTCGCCTACCTGACCGCGCGGATCGGCGACGAGATCGCTCAGATGAAGGGCAAGCG
CGCGCCCTTCGCGCCGCATTGGGCGCTGCCCGACCAGATCGCGCACCGCCTCAAGCGCGAGGTGCCGTTGTGGGCAATCG
GCGCCGTGTTCGCGCTGGTCGGCCTGCTGGCCTTCGTCGGGCTCAACGCCTATCTGCGCGATCACACGCTGAAGGCGCTG
GCGCCCTATTCGCAGATCATCAAGGTCGGGCCGGAATCGGCCAACCTGACGATCTCGCTGCCCTGA