SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP016219
Component protein type	TssL
T6SS ID (Type)	T6SS01299 (Type i1)
Strain	Pseudomonas sp. JY-Q
Replicon	chromosome
Sequence	Protein sequence (290 a.a.); Nucleotide sequence (870 bp)
Reference	[1] Li J, Xie L, Qian S, et al. A Type VI Secretion System Facilitates Fitness, Homeostasis, and Competitive Advantages for Environmental Adaptability and Efficient Nicotine Biodegradation. Appl Environ Microbiol. 2021 Apr 13;87(9). pii: e03113-20. doi: 10.1128/AEM.03113-20. Print 2021 Apr 13. PMID: 33608299

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AA098_RS18555
Coordinate (Strand)	4087932..4088801 (+)
NCBI ID	WP_064614894.1
RefSeq	NZ_CP011525
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	4.4e-60	69..270

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-246	MENSNPTLNDAKHVADTDLHAMLEKSLPNDAPAPATSTASAQEQKPEPAYQGYPADPEFQLRGGCANLMLDAAAPLFGLVMRLRTLDELPNIKDVHQQVRIQIDSIREEIRQHGYEPAQLLAYSYGLCLYIDEAVMDRPWGKSSCWSHEPLLSIFHDETWGGEKFFTVLSRLMQEPKRYQDVLEFMYFALCLGLKGKYAIAPKGEESLNALIHQLHGIIRELRGPTPEEVCDPYTNVAPRNFRMSR
Transmembrane helix	247-269	IWPWWSPLVISAIAMAVAYGIYS
Inside	270-289	YRLHLITTEVLESLNGILQQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9279	-	-

Protein sequence: 290 a.a.

						>T6CP016219 NZ_CP011525:4087932-4088801 [Pseudomonas sp. JY-Q] [TssL]
MENSNPTLNDAKHVADTDLHAMLEKSLPNDAPAPATSTASAQEQKPEPAYQGYPADPEFQLRGGCANLMLDAAAPLFGLV
MRLRTLDELPNIKDVHQQVRIQIDSIREEIRQHGYEPAQLLAYSYGLCLYIDEAVMDRPWGKSSCWSHEPLLSIFHDETW
GGEKFFTVLSRLMQEPKRYQDVLEFMYFALCLGLKGKYAIAPKGEESLNALIHQLHGIIRELRGPTPEEVCDPYTNVAPR
NFRMSRIWPWWSPLVISAIAMAVAYGIYSYRLHLITTEVLESLNGILQQ*					

Nucleotide sequence: 870 bp

						>T6CP016219 NZ_CP011525:4087932-4088801 [Pseudomonas sp. JY-Q] [TssL]
ATGGAAAACAGTAACCCTACCCTCAACGACGCGAAGCACGTTGCGGATACGGATTTGCACGCGATGCTCGAGAAGTCGCT
GCCCAACGACGCACCCGCACCTGCAACTTCCACTGCATCTGCACAGGAGCAGAAGCCGGAGCCTGCATATCAGGGCTACC
CCGCGGATCCGGAGTTTCAGCTCAGGGGTGGCTGCGCCAATCTCATGCTGGATGCCGCGGCGCCCTTGTTCGGGCTGGTC
ATGCGCCTGCGCACGCTCGATGAACTGCCCAACATCAAAGATGTGCATCAGCAAGTACGTATTCAGATCGACAGCATCCG
GGAAGAAATACGCCAGCACGGCTATGAGCCCGCCCAGCTGTTGGCGTACTCCTACGGCCTGTGCCTGTACATCGATGAAG
CGGTGATGGATCGGCCCTGGGGCAAAAGCAGTTGCTGGAGCCACGAGCCGCTGCTCAGCATCTTCCACGATGAAACCTGG
GGCGGGGAAAAATTCTTCACCGTGCTTTCCCGCCTGATGCAGGAGCCCAAGCGCTATCAGGACGTGTTGGAGTTCATGTA
CTTCGCACTGTGCCTGGGTTTGAAGGGCAAGTACGCCATCGCCCCCAAGGGCGAAGAGTCACTGAACGCCCTGATCCACC
AGCTCCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGA
AACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGAT
CTACAGCTACCGCCTGCACCTGATCACCACCGAGGTGCTGGAGTCGCTGAACGGCATCTTGCAGCAATAG					