SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP016214
Component protein type	TssF
T6SS ID (Type)	T6SS01299 (Type i1)
Strain	Pseudomonas sp. JY-Q
Replicon	chromosome
Sequence	Protein sequence (589 a.a.); Nucleotide sequence (1767 bp)
Reference	[1] Li J, Xie L, Qian S, et al. A Type VI Secretion System Facilitates Fitness, Homeostasis, and Competitive Advantages for Environmental Adaptability and Efficient Nicotine Biodegradation. Appl Environ Microbiol. 2021 Apr 13;87(9). pii: e03113-20. doi: 10.1128/AEM.03113-20. Print 2021 Apr 13. PMID: 33608299

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AA098_RS18530
Coordinate (Strand)	4082794..4084560 (+)
NCBI ID	WP_064614887.1
RefSeq	NZ_CP011525
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	2.8e-156	3..585

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-588	MSLKDRFSEELRYLHELGNDFAKDNPQLARLLGKAGSDPDVERLMEAFAFLTAKLRLKLEDDLPELTHPMLQILWPNYLRPLPSATIIRFSPRKQSLSQSQHIPKGARLFSKPVDAVPCEFRTCTGVSLHPFEISAVSATQTLDSSVVRIGLQTLVERPLNTLGCARLDFHLSGDNRTALTLYLWISQYLKHVSVIMNGEVRRLPANSIGFPGFSPEEALLPYPQNVFDGYRILQEYFVFPKRFHFFSITGLEKLWPAQACPQVGIEFHFTRQLPDTLRVGTEDFSLFCTPAVNLFKHSAEPIDLASEAAQVELKPRSEAQSAYEIFSVDEVISTRTTTDGSTGEHLRTFRPFESFAHEIEHVQGRTALYYRCQLEESLRGDGVTHRIAFVRADANSYIGELETASIDLTCTNRDLPLALGVDDINVLTEVTPPLATYTNICAPTRPYRPVLDGQLQWALISNMSLNYLSLLSVEPLKAVIRTYDFAALHDIQQARTTGKRLDGIRDIHTQPMDWLIKGQPIRGLHTQLKLDQAAFLCEGDLYLFSCVLAHFFALYASINSFHQLEVINATNNEHYTWPIQTGKQPLI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9953	-	-

Protein sequence: 589 a.a.

						>T6CP016214 NZ_CP011525:4082794-4084560 [Pseudomonas sp. JY-Q] [TssF]
MSLKDRFSEELRYLHELGNDFAKDNPQLARLLGKAGSDPDVERLMEAFAFLTAKLRLKLEDDLPELTHPMLQILWPNYLR
PLPSATIIRFSPRKQSLSQSQHIPKGARLFSKPVDAVPCEFRTCTGVSLHPFEISAVSATQTLDSSVVRIGLQTLVERPL
NTLGCARLDFHLSGDNRTALTLYLWISQYLKHVSVIMNGEVRRLPANSIGFPGFSPEEALLPYPQNVFDGYRILQEYFVF
PKRFHFFSITGLEKLWPAQACPQVGIEFHFTRQLPDTLRVGTEDFSLFCTPAVNLFKHSAEPIDLASEAAQVELKPRSEA
QSAYEIFSVDEVISTRTTTDGSTGEHLRTFRPFESFAHEIEHVQGRTALYYRCQLEESLRGDGVTHRIAFVRADANSYIG
ELETASIDLTCTNRDLPLALGVDDINVLTEVTPPLATYTNICAPTRPYRPVLDGQLQWALISNMSLNYLSLLSVEPLKAV
IRTYDFAALHDIQQARTTGKRLDGIRDIHTQPMDWLIKGQPIRGLHTQLKLDQAAFLCEGDLYLFSCVLAHFFALYASIN
SFHQLEVINATNNEHYTWPIQTGKQPLI*					

Nucleotide sequence: 1767 bp

						>T6CP016214 NZ_CP011525:4082794-4084560 [Pseudomonas sp. JY-Q] [TssF]
ATGTCACTGAAGGACAGATTCAGCGAAGAACTGCGTTACCTGCACGAGTTGGGAAACGACTTCGCCAAGGACAACCCCCA
GCTTGCCCGGCTGCTAGGCAAGGCGGGCAGCGACCCCGATGTCGAGCGGTTGATGGAAGCCTTTGCGTTTCTAACGGCCA
AGCTTCGGCTGAAGCTGGAAGATGACCTGCCAGAACTCACCCACCCCATGCTGCAGATACTGTGGCCCAACTACCTGCGC
CCGTTGCCCAGCGCAACGATTATCCGATTCTCGCCACGCAAACAATCGCTCAGCCAGTCACAGCACATTCCCAAAGGTGC
AAGGTTGTTCTCGAAACCCGTGGACGCCGTGCCCTGCGAGTTTCGTACCTGCACGGGAGTGAGCCTTCATCCGTTTGAAA
TCAGCGCCGTCAGCGCAACCCAGACACTCGACAGCTCTGTGGTTCGCATCGGCCTGCAAACCTTGGTCGAACGGCCCCTG
AATACGTTGGGCTGCGCCCGTCTGGATTTCCACCTGAGCGGCGACAACCGCACCGCTCTGACGCTTTATCTATGGATTTC
TCAATACCTCAAGCACGTCAGCGTGATCATGAACGGCGAGGTGCGCCGGCTACCGGCCAACAGCATCGGGTTCCCAGGTT
TCAGCCCTGAGGAGGCCTTGCTGCCCTACCCGCAGAACGTGTTCGATGGCTACCGTATTCTGCAGGAATACTTCGTGTTC
CCGAAGCGCTTCCACTTCTTCAGCATTACCGGCCTGGAAAAGCTGTGGCCGGCACAAGCCTGCCCGCAGGTGGGCATCGA
GTTCCATTTCACGCGTCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCA
ACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCG
CAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCT
GCGGACTTTTCGCCCTTTCGAATCGTTCGCCCACGAAATCGAGCATGTCCAAGGCCGCACGGCGCTGTACTACCGCTGCC
AACTCGAAGAGTCCCTGCGCGGCGATGGCGTGACCCACCGCATCGCCTTCGTGCGCGCCGATGCCAACAGCTACATAGGC
GAGCTTGAAACGGCATCCATCGACCTGACCTGCACCAACAGGGACTTGCCGCTGGCGCTGGGCGTGGATGACATCAACGT
CCTCACCGAAGTCACGCCGCCCCTGGCGACCTATACCAACATCTGCGCCCCCACCCGCCCCTACCGGCCCGTGCTGGATG
GCCAGTTGCAGTGGGCGTTGATCTCCAACATGTCGCTCAACTACCTGTCGCTGCTGTCGGTCGAGCCGCTCAAGGCGGTT
ATTCGCACCTACGACTTCGCGGCGTTGCACGACATCCAGCAAGCGCGCACTACCGGCAAACGCCTCGACGGTATCCGCGA
CATCCACACGCAGCCCATGGACTGGCTGATCAAGGGGCAACCCATCCGAGGCCTGCACACCCAACTGAAGCTGGACCAGG
CGGCCTTCCTCTGCGAAGGCGACCTCTACCTGTTCAGCTGCGTGCTCGCGCACTTCTTCGCCCTGTACGCCAGCATCAAT
TCCTTCCATCAACTGGAAGTGATCAACGCCACCAACAACGAGCACTACACATGGCCCATCCAGACCGGCAAGCAACCGCT
GATCTAG					