SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP016207
Component protein type	TssI
T6SS ID (Type)	T6SS01298 (Type i1)
Strain	Pseudomonas sp. JY-Q
Replicon	chromosome
Sequence	Protein sequence (723 a.a.); Nucleotide sequence (2169 bp)
Reference	[1] Li J, Xie L, Qian S, et al. A Type VI Secretion System Facilitates Fitness, Homeostasis, and Competitive Advantages for Environmental Adaptability and Efficient Nicotine Biodegradation. Appl Environ Microbiol. 2021 Apr 13;87(9). pii: e03113-20. doi: 10.1128/AEM.03113-20. Print 2021 Apr 13. PMID: 33608299

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AA098_RS16770
Coordinate (Strand)	3693476..3695644 (+)
NCBI ID	WP_060546558.1
RefSeq	NZ_CP011525
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	4e-70	28..349
Phage_base_V	PF04717.14	1.2e-12	408..475

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-722	MPSQSDLRYSFEALVGNAEFEVVSFELREGISTPFELELKLISFENDIDFGHLLDKPVLFTIWEGGRPVRYVHGLVSRFSQAESGFYRTYYDALIEPQLARASLRSNWRIFQQKTVPQILELMLKRQGIDQYALRASLDHQVREFCVQAGETDLDFIARLAAEEGFVYRFEHNEKRHKLIITDRLQSLGLISHGVIKHEDDAEGFYREREPSATATVLYHSSHGGDQPQPCLRRLRYSEQVRTARQVQRDYTFTHPAYRQEHRASGPYLEHQSTDYECFDYPGRYKRDAVGKPFTENRITGLRHDVRIAQVQGDDVRLQPGLSFTLTGHPREDLNVHWRVSTVRHEGSQFTSLQEEAAGADQGTRYEQKAVLVPGRTEWRPAPLPKPRVDGPHMATVVGPPGEEIYCDEWGRVKVSFPWDRESQDNAFSSCWVRVSQGWAGGSWGSMAIPRIGQDVIIHYVNGDPDQPMITGRTYCGNQLPPYDLPRHKTRMTIKSQTHKGDGFNELRFEDELGQEEVFIHAQRDQNNVVKHDETTQVGNDRKEQVDHDESILIGHDRTETVGNDEHITIGQDAALDIGRNHTIRIAKDRVENIGNHRHDQIAANHHTLIGGNFEQRVEGHAELEARGDIRRRTRRYELRASEAVVIQGPGGSIRIDETGITLDSPTIRITGQMLKSANGTTSPFSISSAPNTGKPLERLCGRRPDGTCPLPDCRCIRGPGQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9967	-	-

Protein sequence: 723 a.a.

						>T6CP016207 NZ_CP011525:3693476-3695644 [Pseudomonas sp. JY-Q] [TssI]
MPSQSDLRYSFEALVGNAEFEVVSFELREGISTPFELELKLISFENDIDFGHLLDKPVLFTIWEGGRPVRYVHGLVSRFS
QAESGFYRTYYDALIEPQLARASLRSNWRIFQQKTVPQILELMLKRQGIDQYALRASLDHQVREFCVQAGETDLDFIARL
AAEEGFVYRFEHNEKRHKLIITDRLQSLGLISHGVIKHEDDAEGFYREREPSATATVLYHSSHGGDQPQPCLRRLRYSEQ
VRTARQVQRDYTFTHPAYRQEHRASGPYLEHQSTDYECFDYPGRYKRDAVGKPFTENRITGLRHDVRIAQVQGDDVRLQP
GLSFTLTGHPREDLNVHWRVSTVRHEGSQFTSLQEEAAGADQGTRYEQKAVLVPGRTEWRPAPLPKPRVDGPHMATVVGP
PGEEIYCDEWGRVKVSFPWDRESQDNAFSSCWVRVSQGWAGGSWGSMAIPRIGQDVIIHYVNGDPDQPMITGRTYCGNQL
PPYDLPRHKTRMTIKSQTHKGDGFNELRFEDELGQEEVFIHAQRDQNNVVKHDETTQVGNDRKEQVDHDESILIGHDRTE
TVGNDEHITIGQDAALDIGRNHTIRIAKDRVENIGNHRHDQIAANHHTLIGGNFEQRVEGHAELEARGDIRRRTRRYELR
ASEAVVIQGPGGSIRIDETGITLDSPTIRITGQMLKSANGTTSPFSISSAPNTGKPLERLCGRRPDGTCPLPDCRCIRGP
GQ*					

Nucleotide sequence: 2169 bp

						>T6CP016207 NZ_CP011525:3693476-3695644 [Pseudomonas sp. JY-Q] [TssI]
ATGCCTAGCCAATCTGATTTGCGCTACAGCTTTGAGGCACTCGTTGGCAACGCTGAGTTCGAAGTGGTGTCGTTCGAACT
ACGAGAAGGGATCAGCACACCCTTCGAACTGGAGCTGAAATTGATCAGCTTCGAGAACGATATCGACTTCGGCCACCTGC
TCGACAAACCGGTGCTCTTCACCATCTGGGAAGGCGGACGCCCAGTACGCTACGTGCACGGTCTGGTCAGTCGTTTCAGC
CAAGCGGAAAGTGGCTTCTACCGCACCTACTATGACGCGCTGATCGAGCCACAGCTGGCCCGTGCCAGCCTGCGTTCGAA
CTGGCGCATCTTCCAGCAGAAGACCGTGCCGCAAATTCTTGAACTGATGCTCAAGCGCCAGGGCATCGACCAGTACGCAC
TACGCGCCAGCCTGGACCACCAGGTGCGCGAATTCTGCGTTCAGGCCGGCGAAACCGACCTCGACTTCATCGCCCGCCTG
GCGGCCGAAGAAGGCTTTGTCTACCGCTTCGAGCACAATGAAAAACGCCATAAGCTGATCATCACCGACAGACTGCAGTC
ACTCGGTCTGATAAGTCACGGTGTCATCAAACACGAGGACGACGCCGAAGGCTTCTACCGTGAGCGTGAACCGTCAGCCA
CAGCCACCGTGCTGTACCATTCCAGCCACGGCGGCGACCAGCCTCAACCGTGCCTACGGCGCTTGCGCTACAGCGAACAG
GTGCGTACTGCACGGCAGGTTCAACGCGACTACACCTTCACCCACCCGGCCTATCGTCAGGAACACAGGGCCTCGGGCCC
GTATCTTGAGCACCAGTCCACGGACTATGAGTGCTTCGACTACCCCGGCCGTTACAAACGCGATGCCGTTGGCAAGCCGT
TCACCGAAAACCGCATCACCGGCTTGCGCCATGACGTGCGTATTGCCCAAGTGCAAGGCGACGATGTGCGCTTGCAACCA
GGCCTGAGCTTCACCCTGACAGGCCACCCGCGCGAAGACCTGAATGTGCATTGGCGGGTCAGCACGGTTCGCCACGAAGG
CAGCCAGTTCACCAGCCTGCAGGAAGAAGCTGCCGGGGCCGACCAGGGCACACGTTACGAGCAAAAGGCGGTACTGGTGC
CAGGCAGAACCGAGTGGCGCCCAGCCCCGTTGCCCAAACCACGCGTTGACGGCCCGCACATGGCCACCGTGGTGGGGCCG
CCCGGTGAAGAGATCTACTGCGACGAATGGGGCCGGGTCAAGGTCAGCTTCCCCTGGGACAGAGAAAGCCAGGACAACGC
GTTCAGTTCCTGCTGGGTACGCGTCTCCCAAGGCTGGGCCGGCGGCAGCTGGGGCTCGATGGCCATCCCACGGATTGGCC
AGGACGTGATCATCCACTACGTCAACGGCGACCCCGACCAGCCGATGATCACCGGCCGTACTTACTGCGGTAACCAGCTG
CCACCGTACGACCTTCCCCGTCACAAGACCCGCATGACCATCAAGAGCCAGACCCACAAGGGCGATGGCTTCAACGAGCT
GCGCTTCGAGGACGAACTGGGCCAGGAGGAAGTGTTCATTCATGCCCAGAGGGACCAGAACAATGTGGTCAAGCACGATG
AGACTACGCAGGTCGGCAATGACCGCAAAGAGCAGGTCGATCACGACGAATCGATCCTGATCGGCCACGACCGTACGGAA
ACGGTTGGCAATGACGAGCACATCACCATCGGGCAGGACGCCGCGCTCGACATCGGCAGAAACCACACGATCAGGATCGC
CAAGGACCGTGTCGAAAACATCGGCAATCACCGTCACGACCAGATCGCCGCCAATCACCACACCCTTATCGGTGGCAACT
TCGAACAACGGGTCGAAGGGCATGCCGAGCTGGAAGCCCGTGGGGATATCCGCAGACGTACTCGCCGTTATGAACTTCGA
GCCTCTGAAGCCGTGGTCATCCAAGGCCCTGGTGGCTCCATTCGAATCGACGAAACGGGCATCACCCTGGACAGCCCGAC
GATTCGCATCACCGGCCAGATGCTCAAGTCGGCAAATGGGACGACAAGCCCCTTTTCCATCAGCAGTGCACCGAACACCG
GCAAGCCTCTGGAGCGCTTGTGTGGGCGCCGCCCTGATGGTACCTGCCCCCTGCCCGATTGCCGCTGTATCCGAGGGCCA
GGCCAATGA					