SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP016205
Component protein type	TssL
T6SS ID (Type)	T6SS01298 (Type i1)
Strain	Pseudomonas sp. JY-Q
Replicon	chromosome
Sequence	Protein sequence (288 a.a.); Nucleotide sequence (864 bp)
Reference	[1] Li J, Xie L, Qian S, et al. A Type VI Secretion System Facilitates Fitness, Homeostasis, and Competitive Advantages for Environmental Adaptability and Efficient Nicotine Biodegradation. Appl Environ Microbiol. 2021 Apr 13;87(9). pii: e03113-20. doi: 10.1128/AEM.03113-20. Print 2021 Apr 13. PMID: 33608299

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AA098_RS16760
Coordinate (Strand)	3691890..3692753 (+)
NCBI ID	WP_060538226.1
RefSeq	NZ_CP011525
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	1.7e-61	67..268

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-245	MGKDNNTPTDENNAAVNALDSTLEKPVPAPRAAATNAQVSTDKPEPFSEGYPADPEFQLRGGFANLMLDAASPLFGLVIRLRTLDELPNIQDVHQQVRGQIDNIQEEMRQHGYEPAQLLAYSYGLCLFIDEAVMERPWGKSSCWSQEPLLSIFHDETWGGEKIFTVITRLMQEPKRYQDVLEFMYYALCLGLKGKYALAPKGEETLNALIHQLHGIIRELRGPTPEEVCDPYTNVAPRNFRMSRQ
Transmembrane helix	246-268	WPWWSPLVISAIAMAVAYSIYSY
Inside	269-287	RLHLITTEVLESLNGILQQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9202	-	-

Protein sequence: 288 a.a.

						>T6CP016205 NZ_CP011525:3691890-3692753 [Pseudomonas sp. JY-Q] [TssL]
MGKDNNTPTDENNAAVNALDSTLEKPVPAPRAAATNAQVSTDKPEPFSEGYPADPEFQLRGGFANLMLDAASPLFGLVIR
LRTLDELPNIQDVHQQVRGQIDNIQEEMRQHGYEPAQLLAYSYGLCLFIDEAVMERPWGKSSCWSQEPLLSIFHDETWGG
EKIFTVITRLMQEPKRYQDVLEFMYYALCLGLKGKYALAPKGEETLNALIHQLHGIIRELRGPTPEEVCDPYTNVAPRNF
RMSRQWPWWSPLVISAIAMAVAYSIYSYRLHLITTEVLESLNGILQQ*					

Nucleotide sequence: 864 bp

						>T6CP016205 NZ_CP011525:3691890-3692753 [Pseudomonas sp. JY-Q] [TssL]
ATGGGAAAAGACAACAATACGCCCACCGATGAAAACAACGCTGCAGTCAACGCGCTTGATTCAACCCTTGAAAAGCCAGT
ACCCGCCCCCCGGGCTGCGGCAACCAATGCGCAAGTATCCACGGACAAACCTGAGCCCTTTTCCGAAGGCTACCCCGCTG
ACCCGGAATTTCAGCTGCGGGGCGGCTTCGCCAACCTGATGCTGGATGCCGCATCGCCGTTGTTCGGCTTGGTTATACGC
CTGCGTACGCTGGACGAGCTGCCAAACATCCAGGACGTGCACCAGCAAGTGCGCGGCCAGATCGACAACATCCAGGAAGA
AATGCGCCAGCACGGTTACGAGCCGGCTCAGTTGCTGGCGTACTCCTACGGTCTGTGCCTGTTCATAGACGAGGCCGTTA
TGGAGCGCCCCTGGGGCAAGAGCAGCTGCTGGAGCCAGGAGCCGCTGCTCAGCATCTTCCACGACGAGACCTGGGGTGGA
GAAAAAATCTTCACGGTGATTACCCGCCTGATGCAGGAGCCCAAGCGTTACCAGGACGTACTGGAGTTCATGTACTACGC
CCTGTGCCTTGGGCTGAAGGGCAAGTACGCGCTCGCGCCGAAAGGCGAAGAAACACTCAACGCACTGATTCACCAACTGC
ACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAATGTGGCCCCACGCAACTTC
CGCATGAGCCGGCAATGGCCCTGGTGGAGCCCCTTGGTGATTTCCGCCATCGCCATGGCGGTGGCTTACAGCATCTATAG
CTATCGCCTGCACCTGATCACAACCGAGGTACTGGAGTCGCTCAACGGCATTTTGCAGCAATAG					