SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP016204
Component protein type	TssK
T6SS ID (Type)	T6SS01298 (Type i1)
Strain	Pseudomonas sp. JY-Q
Replicon	chromosome
Sequence	Protein sequence (446 a.a.); Nucleotide sequence (1338 bp)
Reference	[1] Li J, Xie L, Qian S, et al. A Type VI Secretion System Facilitates Fitness, Homeostasis, and Competitive Advantages for Environmental Adaptability and Efficient Nicotine Biodegradation. Appl Environ Microbiol. 2021 Apr 13;87(9). pii: e03113-20. doi: 10.1128/AEM.03113-20. Print 2021 Apr 13. PMID: 33608299

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AA098_RS16755
Coordinate (Strand)	3690551..3691888 (+)
NCBI ID	WP_064614791.1
RefSeq	NZ_CP011525
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VasE	PF05936.14	4.3e-148	19..442

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-445	MSSRNPVLWPEGLFVKPQHFQQATRASEAALHQRLASLNAAFYGFSELQLSDEYLSLGKVAITRARGVMPDGTVFDIPADLPPPPPLEIGDDACKDSEVYLCLPLRTDGGREVSWPDNAANFRYTAQAQEIKDTHTADGDLVQVDLAVPNLQLKRKTEDSSAYTRLALARILERRPDGSLQLDEAFYPTSVSVRAVPALQRFLEEITHTLRERARGLATRIGASGQSGIADIRDFNLLQAMNRWWPCFQHLARQAQTHPEQLYLCMSQACSEFVTFTDQNRLPQEYPAYHHTALRTSFKPLEDTLRRSLSTVLRPRAVSLPLESEQFGVMTTTLEDRRLIDEADFILAVRASMPPQALRQMFIQKAKITSLESLNDLVPLQLPGIPLLPLPVAPRDLPFHAGFSYFELDRRDPAWACMSKANGFGIHIAGDFPGLELQFWAIRSE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9790	-	-

Protein sequence: 446 a.a.

						>T6CP016204 NZ_CP011525:3690551-3691888 [Pseudomonas sp. JY-Q] [TssK]
MSSRNPVLWPEGLFVKPQHFQQATRASEAALHQRLASLNAAFYGFSELQLSDEYLSLGKVAITRARGVMPDGTVFDIPAD
LPPPPPLEIGDDACKDSEVYLCLPLRTDGGREVSWPDNAANFRYTAQAQEIKDTHTADGDLVQVDLAVPNLQLKRKTEDS
SAYTRLALARILERRPDGSLQLDEAFYPTSVSVRAVPALQRFLEEITHTLRERARGLATRIGASGQSGIADIRDFNLLQA
MNRWWPCFQHLARQAQTHPEQLYLCMSQACSEFVTFTDQNRLPQEYPAYHHTALRTSFKPLEDTLRRSLSTVLRPRAVSL
PLESEQFGVMTTTLEDRRLIDEADFILAVRASMPPQALRQMFIQKAKITSLESLNDLVPLQLPGIPLLPLPVAPRDLPFH
AGFSYFELDRRDPAWACMSKANGFGIHIAGDFPGLELQFWAIRSE*					

Nucleotide sequence: 1338 bp

						>T6CP016204 NZ_CP011525:3690551-3691888 [Pseudomonas sp. JY-Q] [TssK]
ATGAGTTCACGCAATCCCGTTCTCTGGCCCGAAGGCCTGTTCGTCAAACCACAGCATTTCCAGCAGGCGACCAGGGCCAG
CGAAGCAGCCCTGCACCAGCGCCTCGCCAGCCTCAACGCAGCCTTCTACGGCTTCAGCGAACTTCAGTTGAGCGACGAAT
ACCTGAGCCTGGGCAAAGTCGCCATCACCCGGGCGCGAGGTGTGATGCCGGACGGCACGGTGTTCGACATTCCCGCCGAC
CTGCCGCCACCGCCGCCGCTGGAAATCGGCGACGATGCGTGCAAAGACAGTGAGGTGTATCTGTGCCTGCCGCTGCGTAC
CGATGGCGGCCGCGAAGTGAGCTGGCCCGACAATGCAGCCAATTTCCGCTATACCGCCCAGGCACAGGAAATCAAGGATA
CCCACACCGCTGACGGTGACCTGGTGCAGGTAGACCTTGCGGTGCCCAACCTGCAGCTCAAACGCAAGACCGAAGACAGC
AGCGCCTACACCCGCCTGGCCCTGGCACGTATTCTGGAACGGCGCCCCGACGGCAGCCTGCAACTGGACGAAGCGTTCTA
CCCCACCAGCGTTTCGGTGCGGGCGGTGCCAGCGCTGCAGCGGTTTCTTGAAGAAATCACCCATACCTTGCGCGAGCGGG
CGCGCGGCCTCGCCACGCGGATTGGTGCCTCGGGCCAGTCCGGGATCGCCGACATTCGCGACTTCAACCTGCTGCAGGCC
ATGAACCGTTGGTGGCCATGCTTCCAGCATCTGGCCAGGCAAGCGCAAACGCACCCCGAGCAGCTGTACCTGTGCATGAG
CCAGGCCTGCAGCGAATTCGTCACCTTCACCGACCAGAACAGGCTGCCGCAGGAGTACCCCGCCTATCACCACACCGCGC
TGCGCACCTCTTTCAAACCACTGGAAGACACCTTGCGTCGGTCTCTGAGCACCGTCTTGCGGCCACGCGCTGTTTCACTA
CCGCTGGAGAGCGAACAATTCGGCGTGATGACGACCACGCTGGAAGACCGCCGGCTGATCGACGAAGCCGACTTCATCCT
CGCCGTGCGCGCCAGCATGCCACCACAGGCGTTGCGCCAGATGTTCATCCAGAAAGCCAAGATCACCTCGCTTGAGTCAC
TGAATGACCTGGTGCCGCTGCAATTGCCGGGCATCCCTTTGTTGCCATTACCCGTGGCGCCCCGCGACCTGCCCTTCCAT
GCGGGCTTCAGCTACTTCGAGCTGGACCGACGTGACCCGGCCTGGGCGTGCATGAGCAAGGCGAATGGCTTCGGCATCCA
CATTGCGGGCGACTTCCCAGGGCTTGAACTGCAGTTCTGGGCGATCAGGAGTGAGTGA					