SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP016196
Component protein type	TagO
T6SS ID (Type)	T6SS01298 (Type i1)
Strain	Pseudomonas sp. JY-Q
Replicon	chromosome
Sequence	Protein sequence (217 a.a.); Nucleotide sequence (651 bp)
Reference	[1] Li J, Xie L, Qian S, et al. A Type VI Secretion System Facilitates Fitness, Homeostasis, and Competitive Advantages for Environmental Adaptability and Efficient Nicotine Biodegradation. Appl Environ Microbiol. 2021 Apr 13;87(9). pii: e03113-20. doi: 10.1128/AEM.03113-20. Print 2021 Apr 13. PMID: 33608299

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AA098_RS16710
Coordinate (Strand)	3681874..3682524 (-)
NCBI ID	WP_177331243.1
RefSeq	NZ_CP011525
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
VasI	PF11319.10	1.4e-58	27..216

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-216	MINRCAYGAGLSLLFCLQPVLAGSTRDCPRIVSNVERLACFDQAAGTPAYTPKRQWSAPEQQAPTVLRVMANEAERAPDDLTFRIGSQPQDGAGAPALVISAPAIASGDERAYMAISCIQAISRLQLIARRPIEASWVKVRLSGEGWSTRETPWQVMENGQVLDAGRGLPAIEQIRKLIGAQRIEVVSDHGEVDGLTFDAQGLDPLISRARSTCRW

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
SP(Sec/SPI)	0.9497	pos: 22-23. VLA-GS.	MINRCAYGAGLSLLFCLQPVLA

Protein sequence: 217 a.a.

						>T6CP016196 NZ_CP011525:c3682524-3681874 [Pseudomonas sp. JY-Q] [TagO]
MINRCAYGAGLSLLFCLQPVLAGSTRDCPRIVSNVERLACFDQAAGTPAYTPKRQWSAPEQQAPTVLRVMANEAERAPDD
LTFRIGSQPQDGAGAPALVISAPAIASGDERAYMAISCIQAISRLQLIARRPIEASWVKVRLSGEGWSTRETPWQVMENG
QVLDAGRGLPAIEQIRKLIGAQRIEVVSDHGEVDGLTFDAQGLDPLISRARSTCRW*					

Nucleotide sequence: 651 bp

						>T6CP016196 NZ_CP011525:c3682524-3681874 [Pseudomonas sp. JY-Q] [TagO]
ATGATTAACCGCTGTGCATATGGCGCTGGTCTCTCGTTGCTGTTTTGTCTGCAGCCGGTGCTGGCAGGCTCCACGCGGGA
CTGTCCCCGGATCGTGTCCAACGTCGAGCGACTGGCCTGTTTCGATCAGGCTGCTGGCACCCCGGCGTACACGCCAAAAC
GGCAGTGGTCTGCACCGGAGCAGCAGGCCCCCACGGTGTTGCGTGTGATGGCAAATGAAGCTGAACGGGCACCGGATGAC
CTGACGTTTCGCATCGGGAGCCAGCCTCAGGACGGGGCAGGCGCACCAGCGCTTGTGATTTCGGCGCCCGCCATCGCATC
GGGTGATGAACGTGCCTACATGGCAATCAGTTGCATACAGGCCATCTCCCGGCTGCAACTGATTGCCAGGCGTCCGATCG
AAGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGC
CAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGT
GAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGACCCCTTGATCAGCCGGGCGCGAAGCACAT
GTCGGTGGTAG					