SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP016193
Component protein type	TssB
T6SS ID (Type)	T6SS01297 (Type i4b)
Strain	Pseudomonas sp. JY-Q
Replicon	chromosome
Sequence	Protein sequence (180 a.a.); Nucleotide sequence (540 bp)
Reference	[1] Li J, Xie L, Qian S, et al. A Type VI Secretion System Facilitates Fitness, Homeostasis, and Competitive Advantages for Environmental Adaptability and Efficient Nicotine Biodegradation. Appl Environ Microbiol. 2021 Apr 13;87(9). pii: e03113-20. doi: 10.1128/AEM.03113-20. Print 2021 Apr 13. PMID: 33608299

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AA098_RS13310
Coordinate (Strand)	2929989..2930528 (-)
NCBI ID	WP_003248358.1
RefSeq	NZ_CP011525
Uniprot ID	I7C5B0_PSEPT, A0A2I0CHH4_9PSED, A0A173HMP2_9PSED, A0A2K4LNU7_9PSED, A0A1X0ZU83_PSEPU, A5W3P6_PSEP1, A0A7L9GCM4_9PSED
KEGG ID	ppx:T1E_2459, ppf:Pput_2622
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	5.4e-62	12..164

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-179	MAKKESTQHKLDRVRAPRVHITYDVDIGDAIEKKELPFVVGVLGDFSGNPLEPLPKLKDRKFVYIDRDNFNGVLKGIKPRLTYRVDNTLAKNGTQLGVELNFNSLEDFEPQNVVKQVEPLRKLLEVRNKLADLRNKMGGNDKLEELLMDVLQNTEKLKTLGKEFGREAEVKDPSSDDRA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9966	-	-

Protein sequence: 180 a.a.

						>T6CP016193 NZ_CP011525:c2930528-2929989 [Pseudomonas sp. JY-Q] [TssB]
MAKKESTQHKLDRVRAPRVHITYDVDIGDAIEKKELPFVVGVLGDFSGNPLEPLPKLKDRKFVYIDRDNFNGVLKGIKPR
LTYRVDNTLAKNGTQLGVELNFNSLEDFEPQNVVKQVEPLRKLLEVRNKLADLRNKMGGNDKLEELLMDVLQNTEKLKTL
GKEFGREAEVKDPSSDDRA*					

Nucleotide sequence: 540 bp

						>T6CP016193 NZ_CP011525:c2930528-2929989 [Pseudomonas sp. JY-Q] [TssB]
ATGGCGAAGAAGGAAAGCACTCAGCACAAACTCGACCGCGTTCGCGCGCCTCGGGTTCATATCACCTACGACGTGGACAT
CGGCGATGCCATCGAGAAAAAGGAGCTGCCCTTCGTCGTCGGCGTACTGGGTGATTTCTCCGGCAACCCGCTGGAGCCGC
TGCCCAAGCTCAAGGACCGCAAGTTCGTGTACATCGACCGCGACAACTTCAACGGTGTGCTTAAGGGCATCAAGCCACGC
CTGACCTACCGCGTCGACAACACCCTGGCCAAGAACGGTACCCAGCTGGGCGTGGAGCTGAACTTCAACAGCCTCGAAGA
CTTCGAGCCACAGAACGTGGTCAAGCAGGTCGAGCCGCTGCGCAAGCTGCTGGAAGTGCGCAACAAGCTGGCCGACCTGC
GCAACAAGATGGGCGGCAACGACAAGCTCGAAGAGCTGCTGATGGATGTGCTGCAGAACACCGAAAAGCTCAAGACCTTG
GGCAAGGAATTCGGCCGTGAAGCCGAAGTGAAAGACCCGTCCAGCGACGATCGCGCCTGA					