SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP016189
Component protein type	TssG
T6SS ID (Type)	T6SS01297 (Type i4b)
Strain	Pseudomonas sp. JY-Q
Replicon	chromosome
Sequence	Protein sequence (357 a.a.); Nucleotide sequence (1071 bp)
Reference	[1] Li J, Xie L, Qian S, et al. A Type VI Secretion System Facilitates Fitness, Homeostasis, and Competitive Advantages for Environmental Adaptability and Efficient Nicotine Biodegradation. Appl Environ Microbiol. 2021 Apr 13;87(9). pii: e03113-20. doi: 10.1128/AEM.03113-20. Print 2021 Apr 13. PMID: 33608299

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AA098_RS13290
Coordinate (Strand)	2925109..2926179 (-)
NCBI ID	WP_003248350.1
RefSeq	NZ_CP011525
Uniprot ID	A0A2I0CH70_9PSED, A0A173HFK0_9PSED, Q88IA3_PSEPK, A0A1Y5KXG0_PSEPU, A0A2V4FJR9_9PSED
KEGG ID	ppu:PP_3096
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	6.2e-104	19..333

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-356	MASTHRRSAPGLIDQARAEPHRFEFFQLVRLLRLHYSRTGRMDLATRPHEDPLRFRTQLSLAFPASEVSDLHFEREGKVSPAGLPLSEVQVTFMGLVGPSGVLPRPYTELLLERHVQYRDDAAHAFLDIFSHRMTTLFYEAWQKYKFHIEHERNGTSGFDGYLLNLVGLGPRAQKLKFEEQPSALRRELFSYFAGLLSQKPRNALNLERMLGFYFSLPIKVQPFAGRWLHLQPEQCTQLGRRNAQLGHSAVAGNRVWDYQSCIRIELGPLSLADYQRFQPGTACYRKLVELVRFYIGAELDFEIAPRLKREAVPRAQLGRSGNVALGWLGWLKRPGRDAEPSRCAVFHIPYDGVAL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9820	-	-

Protein sequence: 357 a.a.

						>T6CP016189 NZ_CP011525:c2926179-2925109 [Pseudomonas sp. JY-Q] [TssG]
MASTHRRSAPGLIDQARAEPHRFEFFQLVRLLRLHYSRTGRMDLATRPHEDPLRFRTQLSLAFPASEVSDLHFEREGKVS
PAGLPLSEVQVTFMGLVGPSGVLPRPYTELLLERHVQYRDDAAHAFLDIFSHRMTTLFYEAWQKYKFHIEHERNGTSGFD
GYLLNLVGLGPRAQKLKFEEQPSALRRELFSYFAGLLSQKPRNALNLERMLGFYFSLPIKVQPFAGRWLHLQPEQCTQLG
RRNAQLGHSAVAGNRVWDYQSCIRIELGPLSLADYQRFQPGTACYRKLVELVRFYIGAELDFEIAPRLKREAVPRAQLGR
SGNVALGWLGWLKRPGRDAEPSRCAVFHIPYDGVAL*					

Nucleotide sequence: 1071 bp

						>T6CP016189 NZ_CP011525:c2926179-2925109 [Pseudomonas sp. JY-Q] [TssG]
ATGGCCAGCACGCACCGGCGATCAGCCCCTGGTCTGATCGATCAGGCCCGAGCCGAGCCGCACCGATTCGAATTCTTTCA
ATTGGTGCGCCTGCTCCGCCTGCACTACAGCCGCACCGGGCGCATGGACCTGGCAACCCGGCCGCACGAAGACCCGCTGC
GTTTTCGTACCCAGTTGTCGCTGGCGTTCCCGGCCAGCGAGGTCAGCGACCTGCACTTCGAGCGCGAAGGCAAGGTGTCG
CCGGCCGGCTTGCCGCTGTCGGAAGTGCAGGTCACCTTCATGGGCCTGGTCGGGCCGTCGGGCGTGCTGCCACGCCCGTA
CACCGAGCTGCTGCTCGAGCGCCACGTGCAGTATCGCGACGATGCCGCCCATGCGTTTCTCGACATCTTCTCGCACCGCA
TGACCACGCTGTTCTACGAGGCCTGGCAAAAATACAAATTCCACATCGAGCACGAGCGCAATGGCACTTCGGGCTTCGAC
GGCTACCTGCTCAACCTGGTTGGCCTCGGGCCGCGCGCCCAGAAGCTGAAGTTCGAAGAGCAACCGTCAGCATTGCGTCG
TGAGCTGTTCAGCTATTTTGCCGGCCTGCTCAGCCAGAAACCGCGCAATGCGCTGAACCTTGAACGCATGCTCGGTTTCT
ATTTCTCGCTGCCGATCAAGGTCCAGCCGTTCGCCGGGCGCTGGCTGCACCTGCAGCCCGAACAATGCACCCAGCTTGGC
CGCCGCAATGCCCAGCTCGGCCACAGCGCCGTCGCCGGCAACCGGGTCTGGGACTACCAGTCGTGCATCCGCATCGAGCT
GGGCCCGCTGAGCCTGGCCGACTACCAGCGCTTCCAGCCGGGCACCGCCTGCTACCGCAAGCTGGTCGAGCTCGTGCGCT
TCTATATAGGTGCCGAGCTGGATTTCGAAATTGCCCCGCGCCTCAAGCGCGAGGCGGTGCCACGCGCCCAACTGGGCCGC
AGCGGCAATGTCGCCCTGGGCTGGCTGGGTTGGCTCAAACGCCCGGGTCGTGACGCGGAGCCTTCGCGTTGCGCCGTCTT
CCACATTCCTTACGATGGGGTCGCCTTGTGA					