Detailed information of component protein


Summary

Component ID T6CP016182
Component protein type TssM
T6SS ID (Type) T6SS01297 experimental (Type i4b)
Strain Pseudomonas sp. JY-Q
Replicon chromosome
Sequence Protein sequence (832 a.a.); Nucleotide sequence (2496 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) AA098_RS13255
Coordinate (Strand) 2912484..2914979 (-)
NCBI ID WP_064614358.1
RefSeq NZ_CP011525
Uniprot ID A0A173HEY6_9PSED
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 8.6e-75 189..446
IcmF-related PF06761.14 7.2e-17 498..697
OmpA PF00691.22 6.3e-14 716..807
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 MNKLKYYCLRYQ
Transmembrane helix 13-35 PYLLGVAFFLAIFLVWAIGRAFG
Outside 36-38 FAS
Transmembrane helix 39-61 LHSLLAGIGVFLLLSAGYVLLLY
Inside 62-442 RGVGQHHNLEGLLRDDADQAVLSAAPADREEVSLLRERLLQGIERLHSNKPRGTSNKDALYALPWYLVIGQPAAGKSTMILQSGLNFPYAEREGVRVAGLGGTRNCDWFFSSEAVLLDTAGRYMNSPEEAGKWRGFLQLLRQHRQRRPLNGLIVSVSIADILHGSVEDQERVAKRLRERIQESCALLEVRLPIYLVFTKCDLIPGFTAFYRQLDDAARGEVMGKTFSHKGYEQADWGQRFSNAMDELTRYWGQMASQQLVQQDIQVTRQNNAAYRFPLELAALKPRLHQFVDSLLRANPYQNAEMLRGFYFTAALQADEAQWGCHGQHVAERFALEHAEGTGTAGSQPAPLFINSLFRKVIIPDQHLVALYTSNHRERRRK
Transmembrane helix 443-465 AGWVGAAGLAALVLCSLWGWSYL
Outside 466-831 NNRATLASIADELAQAKADDQAASGQYTAWRSLDRLRFWAAHYHEQHHAKGVPLSMRLGLYQGHEVEPLLRNRYFAMLQNVMLKPTADNLTRTLYLLTTLKVYQRNTRELQPVSGIDSVEAEALPHDNRAQSIANFGKAALDTYVMLSPGQREHADPAFLKAHLPDYWYPAIARHTGKSMAAAGSEAGGNQDYLYASRQITFYSDQIREPDVPRILDNAFLMSSSRNYIDSLRAQSLRAIETITLESDTLFAFGRADFQSLKNEGQHQLSAIASKLLNTPNIGKIIISGHADQLGDSQGNLQVSKQRAQTIRTYLVGKGVPGELVVAQGEGSRKPLVNCDMQQPRTQLIKCLEPNRRVEIEVRGLN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9898 - -


  Protein sequence: 832 a.a.    

>T6CP016182 NZ_CP011525:c2914979-2912484 [Pseudomonas sp. JY-Q] [TssM]
MNKLKYYCLRYQPYLLGVAFFLAIFLVWAIGRAFGFASLHSLLAGIGVFLLLSAGYVLLLYRGVGQHHNLEGLLRDDADQ
AVLSAAPADREEVSLLRERLLQGIERLHSNKPRGTSNKDALYALPWYLVIGQPAAGKSTMILQSGLNFPYAEREGVRVAG
LGGTRNCDWFFSSEAVLLDTAGRYMNSPEEAGKWRGFLQLLRQHRQRRPLNGLIVSVSIADILHGSVEDQERVAKRLRER
IQESCALLEVRLPIYLVFTKCDLIPGFTAFYRQLDDAARGEVMGKTFSHKGYEQADWGQRFSNAMDELTRYWGQMASQQL
VQQDIQVTRQNNAAYRFPLELAALKPRLHQFVDSLLRANPYQNAEMLRGFYFTAALQADEAQWGCHGQHVAERFALEHAE
GTGTAGSQPAPLFINSLFRKVIIPDQHLVALYTSNHRERRRKAGWVGAAGLAALVLCSLWGWSYLNNRATLASIADELAQ
AKADDQAASGQYTAWRSLDRLRFWAAHYHEQHHAKGVPLSMRLGLYQGHEVEPLLRNRYFAMLQNVMLKPTADNLTRTLY
LLTTLKVYQRNTRELQPVSGIDSVEAEALPHDNRAQSIANFGKAALDTYVMLSPGQREHADPAFLKAHLPDYWYPAIARH
TGKSMAAAGSEAGGNQDYLYASRQITFYSDQIREPDVPRILDNAFLMSSSRNYIDSLRAQSLRAIETITLESDTLFAFGR
ADFQSLKNEGQHQLSAIASKLLNTPNIGKIIISGHADQLGDSQGNLQVSKQRAQTIRTYLVGKGVPGELVVAQGEGSRKP
LVNCDMQQPRTQLIKCLEPNRRVEIEVRGLN*

  Nucleotide sequence: 2496 bp    

>T6CP016182 NZ_CP011525:c2914979-2912484 [Pseudomonas sp. JY-Q] [TssM]
ATGAACAAGCTCAAGTACTACTGCCTGCGCTACCAACCCTACCTGCTGGGCGTGGCGTTCTTCCTGGCGATTTTCCTGGT
CTGGGCCATCGGCCGAGCCTTCGGCTTCGCCTCGCTGCACAGCCTGCTGGCCGGGATCGGCGTGTTTCTGCTGCTGTCGG
CGGGCTATGTACTGCTGCTGTACCGCGGAGTGGGTCAGCACCACAACCTTGAAGGCCTGCTGCGCGACGACGCCGACCAG
GCTGTACTCAGTGCAGCCCCGGCCGACCGGGAAGAAGTCAGCCTGTTGCGCGAGCGCCTGCTGCAGGGCATCGAGCGCCT
GCACAGCAACAAGCCGCGCGGTACCTCGAACAAGGATGCACTGTATGCCCTGCCCTGGTACCTGGTCATTGGCCAGCCTG
CCGCCGGCAAGAGCACGATGATCCTGCAGTCGGGCCTGAACTTCCCCTATGCCGAGCGCGAAGGCGTGCGGGTCGCGGGC
CTGGGCGGCACGCGCAACTGCGACTGGTTCTTCAGTTCTGAAGCGGTACTGCTGGACACTGCCGGGCGCTACATGAACAG
CCCCGAGGAAGCCGGCAAGTGGCGCGGCTTTTTGCAACTGCTGCGCCAGCATCGCCAGCGCCGCCCCCTTAACGGCTTGA
TCGTCAGCGTCAGCATCGCCGATATCCTGCATGGCTCGGTCGAAGACCAGGAGCGCGTGGCCAAGCGCCTGCGCGAACGC
ATTCAGGAAAGCTGCGCGCTGCTTGAAGTGCGCCTGCCAATCTACCTGGTTTTCACCAAGTGCGACCTGATCCCTGGTTT
CACCGCGTTCTATCGCCAGCTGGACGACGCCGCGCGTGGTGAAGTGATGGGTAAAACCTTCTCTCACAAGGGGTACGAGC
AAGCCGACTGGGGCCAGCGCTTCAGCAATGCGATGGACGAGCTGACCCGTTACTGGGGGCAGATGGCGAGCCAGCAGCTG
GTGCAGCAGGACATACAAGTCACTCGGCAGAACAACGCCGCCTACCGCTTCCCGCTGGAGCTGGCCGCGCTCAAGCCGCG
CCTGCATCAGTTCGTCGACAGCCTGCTGCGTGCCAACCCTTACCAGAATGCCGAAATGCTGCGCGGTTTCTATTTTACCG
CGGCGCTGCAGGCCGACGAGGCTCAGTGGGGCTGCCACGGCCAGCATGTGGCCGAACGCTTCGCGCTCGAACACGCAGAA
GGAACGGGCACTGCTGGCAGCCAGCCGGCGCCGCTGTTCATCAACAGCCTGTTCCGCAAAGTGATCATTCCCGACCAGCA
TTTGGTGGCGCTGTACACCAGCAACCACCGTGAACGCCGGCGCAAGGCCGGGTGGGTGGGCGCTGCCGGCCTGGCGGCAC
TGGTGCTGTGCAGCCTGTGGGGCTGGTCGTACCTGAACAACCGCGCCACCCTGGCCAGCATTGCCGACGAACTGGCCCAG
GCCAAGGCCGACGACCAGGCGGCCAGCGGCCAGTACACCGCCTGGCGAAGCCTCGACCGCTTGCGCTTCTGGGCTGCTCA
CTACCATGAGCAACACCACGCAAAGGGCGTGCCCCTCAGCATGCGCCTGGGCCTGTACCAGGGCCATGAGGTCGAGCCGC
TGCTGCGCAACCGCTACTTCGCCATGTTGCAGAACGTGATGCTAAAGCCCACGGCCGATAATCTGACCCGCACCCTGTAC
CTGCTGACCACGCTCAAGGTCTACCAGCGCAACACCCGTGAGCTGCAGCCAGTGAGCGGCATTGACAGCGTAGAGGCCGA
GGCGCTGCCCCATGACAACCGCGCCCAGTCGATCGCCAATTTCGGAAAGGCTGCCCTGGACACTTACGTGATGCTTTCTC
CGGGCCAGCGCGAGCATGCCGACCCGGCGTTTCTCAAGGCGCACCTGCCGGACTACTGGTACCCGGCCATCGCCCGCCAT
ACCGGCAAGAGCATGGCCGCCGCTGGCAGCGAGGCCGGTGGCAACCAGGACTACCTGTATGCCAGCCGCCAGATCACGTT
CTATAGCGACCAGATCCGTGAGCCAGATGTACCGCGCATCCTCGACAACGCCTTCCTGATGTCCAGTTCGCGCAACTACA
TCGACAGCCTGCGGGCCCAGTCACTGCGTGCGATCGAGACCATCACGCTGGAATCCGACACCCTGTTCGCCTTCGGCCGC
GCCGATTTCCAGAGCCTGAAAAACGAAGGCCAGCATCAGCTGAGCGCAATTGCCAGCAAGCTGCTGAACACCCCGAATAT
CGGCAAGATCATCATCTCGGGGCACGCCGACCAACTGGGTGACAGCCAGGGCAACCTGCAGGTTTCGAAACAACGGGCGC
AAACCATCCGCACCTATCTGGTTGGCAAGGGCGTACCTGGCGAGCTGGTGGTCGCGCAGGGCGAAGGCAGCCGCAAGCCA
TTGGTCAACTGCGACATGCAACAACCGCGAACGCAGTTGATCAAGTGCCTGGAGCCCAACCGCCGGGTGGAAATCGAAGT
GCGTGGGCTTAACTGA