Detailed information of component protein


Summary

Component ID T6CP016163
Component protein type Vt6A
T6SS ID (Type) T6SS00947 experimental (Type Unknown)
Strain Escherichia coli strain PE050
Replicon chromosome I
Sequence Protein sequence (827 a.a.); Nucleotide sequence (2481 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) -
Coordinate (Strand) 1..2481 (+)
NCBI ID 1062685202
GenBank KX118292
Uniprot ID A0A0J2B8M5_ECOLX
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 3.1e-58 30..345
DUF2345 PF10106.11 1.3e-48 603..743
T6SS_Vgr PF13296.8 1.1e-33 484..585
Phage_base_V PF04717.14 1.7e-09 397..463
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-826 MNVQFFDHAHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQFTSSDKAIAPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEVRLEPRMALLARSRQNAIYQNLTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQVMQYGEDDLTFISRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGLFDGETEAVWGLNTAYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYADNFLQKGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGDDAPAVFRKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPRPVMGGTLPARVTSTVKNDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQLNLGHLVDAGKQQRGEGFELRTDMWGVVRAKKGIFISADAQDNAQGQVREMAPAMAILNGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLNQAAILLSAPKGVAIASGEHLQLAAAKNVIANAGNNADVGVVKNMFIGVGQALSVFVRKAGIKLFANKGAISVQAQNDLMELLAKKEIIITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEYNVKAGYYGRKPKAKLTPELMAFPVIEGGDYSRCFKFFDDENIPYRNVKYIANFIDGTKKEGVTDDEGCTETFFSDKEEEIEIEILLGV


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9926 - -


  Protein sequence: 827 a.a.    

>T6CP016163 KX118292:1-2481 [Escherichia coli] [Vt6A]
MNVQFFDHAHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQFTSSDKAIAPESVLMQDGAFSLTAPPVQGMPVQTALRT
LHGVITGFKHLSSSQDEARYEVRLEPRMALLARSRQNAIYQNLTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQVMQ
YGEDDLTFISRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGLFDGETEAVWGLNTAYSVVEKSVTT
RDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYADNFLQKGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGL
EIRVQGDDAPAVFRKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPRPVMGGTLPARVTSTVKNDIYAHIDKD
GRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKR
NVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQLNLGHLVDAGKQQRGEGFELRTDMWGVVRAKKGIFISADAQDNAQGQ
VREMAPAMAILNGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLNQAAILLSAPKGVAIASGEHLQLAAAKNVIA
NAGNNADVGVVKNMFIGVGQALSVFVRKAGIKLFANKGAISVQAQNDLMELLAKKEIIITSTEDEIKITAKKKITLNGGG
SYIRLDACGIEAGTPGEYNVKAGYYGRKPKAKLTPELMAFPVIEGGDYSRCFKFFDDENIPYRNVKYIANFIDGTKKEGV
TDDEGCTETFFSDKEEEIEIEILLGV*

  Nucleotide sequence: 2481 bp    

>T6CP016163 KX118292:1-2481 [Escherichia coli] [Vt6A]
ATGAACGTACAGTTCTTCGACCACGCACACCACAAGCTCAAAATTCGCGGCCTGCAAAGTCCGGTGGATGTGCTGACCTT
TGAAGGCCGCGAGCAACTGAGTACCCCGTTCCGCTACGACATTCAGTTTACCAGCAGCGATAAAGCCATCGCGCCGGAGT
CGGTGCTGATGCAGGATGGCGCATTCAGCCTGACGGCACCGCCGGTACAGGGAATGCCGGTACAGACTGCCCTGCGCACC
CTGCACGGCGTCATTACCGGCTTTAAACATCTCTCATCGTCACAGGACGAAGCCCGTTACGAAGTACGGCTGGAGCCGCG
TATGGCGCTGCTGGCCCGCAGCCGCCAGAATGCCATCTACCAGAATCTGACGGTGCCGCAAATCGTCGAAAAAATCCTGC
GCGAGCGGCACCAGATGCGCGGTCAGGACTTCGTGTTTAACCTCAAAAGCGAATACCCGGCCCGCGAACAGGTGATGCAG
TATGGCGAGGACGACCTGACGTTTATTTCACGCCTGCTGTCGGAAGTGGGCATCTGGTTCCGCTTCGCCACCGACGCCCG
GCTGAAAATTGAGGTTGTCGAGTTTTATGACGACCAGAGCGGCTATGAGCGCGGGCTGACGCTGCCGCTTCGCCATCCTT
CAGGGCTGTTTGATGGTGAAACGGAAGCCGTATGGGGGCTGAACACCGCGTACAGCGTGGTGGAAAAAAGCGTCACCACG
CGGGACTACAACTACCGCACCGCCACAGCGGAGATGATGACAGAGCAGCATGACGCCACCGGGGGCGATAACACCACCTA
CGGCGAAGCCTATCACTACGCCGACAACTTCCTGCAAAAAGGCGATAAAGAGGCAGCGGAAAGCGGCGCGTTTTATGCCC
GTATCCGCCACGAACGCTACCTGAATGAACAGGCCATTCTCAAAGGCCAGAGCACCAGTTCACTGCTGATGCCGGGGCTG
GAAATCAGGGTTCAGGGCGACGATGCGCCAGCGGTGTTTCGCAAGGGCGTGCTGATTACCGGCGTGACGGCCAGCGCGGC
GCGTGACCGCAGCTATGAACTGACGTTTACCGCCATCCCGTACTCAGAACGCTACGGCTACCGCCCGGCGCTTATCCCCC
GCCCCGTAATGGGCGGCACGCTCCCGGCGCGGGTCACCAGCACGGTGAAGAACGATATTTATGCCCATATCGACAAGGAC
GGGCGCTACCGGGTCAACCTCGACTTTGACCGCGATACGTGGAAACCGGGTTATGAAAGCCTGTGGGTACGCCAGTCGCG
CCCGTATGCGGGAGACACCTACGGCCTGCACCTGCCGCTGCTGGCGGGAACGGAAGTCAGTATCGCCTTTGAGGAAGGCA
ACCCGGACCGACCTTATATTGCAGGCGTAAAACACGACTCGGCACACACTGACCATGTGACCATTCAGAACTATAAGCGT
AACGTCCTCAGAACCCCGGCGAACAACAAAATCCGCCTCGACGATGAGCGCGGCAAAGAACATATCAAGGTCAGTACCGA
GTACGGCGGCAAGAGCCAGCTAAACCTCGGTCATCTGGTGGATGCCGGGAAACAGCAACGCGGCGAAGGCTTTGAACTGC
GTACCGATATGTGGGGCGTGGTGCGGGCAAAGAAAGGCATCTTTATCAGCGCCGATGCGCAGGATAATGCGCAGGGGCAG
GTGCGCGAAATGGCTCCTGCAATGGCGATACTGAATGGCGCACAGTCACAGATGAAGTCTCTGTCCACTGATGCGCAGAC
GGCGAATGCCGATCCGGCAGATCTGTCATCGCAGATTGCGTTGTTGCAACAGAGCGTAAAAGATCTGAACCAGGCTGCGA
TCCTGCTAAGCGCACCCAAGGGCGTGGCGATCGCCAGCGGCGAGCACCTGCAACTGGCGGCGGCAAAGAATGTGATAGCC
AACGCAGGCAACAACGCCGATGTCGGCGTGGTGAAAAATATGTTTATCGGCGTGGGACAGGCGCTGAGCGTGTTTGTGCG
TAAAGCGGGCATCAAGCTGTTTGCCAATAAGGGCGCAATCTCGGTGCAGGCGCAGAACGACTTGATGGAACTGTTGGCGA
AGAAGGAAATCATCATTACCAGTACCGAAGATGAAATTAAAATCACGGCGAAGAAAAAGATCACCCTTAACGGCGGTGGC
AGCTACATCCGGCTGGATGCCTGTGGGATAGAAGCGGGAACGCCGGGAGAATATAACGTTAAGGCGGGATATTATGGGCG
TAAGCCGAAGGCGAAGCTGACGCCGGAGCTGATGGCGTTTCCGGTGATTGAGGGTGGAGACTATTCTCGCTGTTTTAAAT
TTTTTGATGACGAAAATATCCCATATCGTAATGTTAAGTATATAGCCAATTTTATTGATGGAACAAAAAAGGAAGGTGTC
ACTGATGATGAGGGATGCACCGAAACATTTTTTAGTGATAAAGAAGAAGAAATTGAAATTGAGATTTTACTTGGTGTTTA
A