Detailed information of component protein


Summary

Component ID T6CP016162
Component protein type Vt4E
T6SS ID (Type) T6SS00946 experimental (Type Unknown)
Strain Escherichia coli strain PE117
Replicon chromosome I
Sequence Protein sequence (1109 a.a.); Nucleotide sequence (3327 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) -
Coordinate (Strand) 5040..8366 (+)
NCBI ID 1062685233
GenBank KX118293
Uniprot ID A0A0F3TBD8_ECOLX
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 2e-57 394..709
IcmF_C PF06744.14 1.3e-23 965..1069
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-30 MTVAVVVALCTFIHRKPALLGVEPGSSQQM
Transmembrane helix 31-53 YWMAGVIAAGVVFLIIMGVYHLL
Inside 54-339 NRHAAVQNFELRRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGPFWRRNVRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSAEPDVTLLTALKKLRRSRPLDGIIWALTEEQSRQTAQLDKGWRELINGGKRLGFQAPLYLWQVCDNGDYQAGRPLQSVGCLLPERCTPEQLTAMLEAHTLPLTEQGMSQLLADNRHDFLLRLAHTLAERGIAHWQSVLKPLLAGGAFSSLRLRGLMFSPPLTAVPEAAPHAWLPSQVWAGVTGDNARGRAVG
Transmembrane helix 340-362 FPWLRAALMSAVCVLAIWGAGMT
Outside 363-1108 TSFFANCALVQETGIQTARALDTRLPLAEQLVALHTLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVDHLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADSLRYENIPEGVQQSVFPSLLTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTLYQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVTARHEEIDWVLSDRQQDTSADISPDTLRNRLTSRYFTDFTGSWLAFLNSIRWKKEDSLSGILDQLTLMADARQSPLIALTDTLAWQAAAGRENRGLSDSLAKSAQELFNGKEKTPQQSREGDVPVGPLDKTFAPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAPDPQEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQVLTPAADSLNRQWQRAIVSHWNQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAANLSGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELQAKPARDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRGSWSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPGQVFLTGRSMKDAEEYGEDADE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9900 - -


  Protein sequence: 1109 a.a.    

>T6CP016162 KX118293:5040-8366 [Escherichia coli] [Vt4E]
MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFLIIMGVYHLLNRHAAVQNFELRRQLAAGDDYRAVTTK
KADSRHPAMVELAAFLRDRYGPFWRRNVRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSAEPDVTLLTALKK
LRRSRPLDGIIWALTEEQSRQTAQLDKGWRELINGGKRLGFQAPLYLWQVCDNGDYQAGRPLQSVGCLLPERCTPEQLTA
MLEAHTLPLTEQGMSQLLADNRHDFLLRLAHTLAERGIAHWQSVLKPLLAGGAFSSLRLRGLMFSPPLTAVPEAAPHAWL
PSQVWAGVTGDNARGRAVGFPWLRAALMSAVCVLAIWGAGMTTSFFANCALVQETGIQTARALDTRLPLAEQLVALHTLQ
GELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVDHLQQQLNAFVALPPNSPQRTATGEQRYKQL
KALLMTSRPEKADAAFFSTTLMADSLRYENIPEGVQQSVFPSLLTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVR
NAENTLYQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVTARHEEIDWVLSDRQQD
TSADISPDTLRNRLTSRYFTDFTGSWLAFLNSIRWKKEDSLSGILDQLTLMADARQSPLIALTDTLAWQAAAGRENRGLS
DSLAKSAQELFNGKEKTPQQSREGDVPVGPLDKTFAPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAPDPQE
MTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQVLTPAADSLNRQWQRAIVSHWNQDFAG
RYPFKASQNDASLPLLAQYLRDDGRINQFIAANLSGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGD
AGIHFELQAKPARDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRGSWSLIRLLEQA
QVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPGQVFLTGRSMKDAEEYGEDADE*

  Nucleotide sequence: 3327 bp    

>T6CP016162 KX118293:5040-8366 [Escherichia coli] [Vt4E]
ATGACCGTGGCTGTTGTGGTTGCGCTTTGCACGTTTATTCACCGTAAACCGGCATTGCTGGGCGTTGAACCGGGTAGCTC
ACAGCAAATGTACTGGATGGCTGGCGTGATTGCCGCAGGCGTGGTCTTTTTGATTATCATGGGGGTTTATCATCTGCTGA
ACCGCCACGCTGCCGTACAAAACTTTGAACTCCGCAGACAACTTGCCGCCGGAGATGACTATCGTGCGGTGACGACAAAG
AAAGCCGACAGCCGTCATCCGGCAATGGTGGAACTCGCCGCCTTCCTGCGCGACCGCTACGGTCCGTTCTGGCGGCGTAA
CGTCCGCCTGCTGCTGGTGACCGGCGAGCCTGAACAGGCAGAAGCCATCGCGCCGGGGCTGACCGGGCAACACTGGCTGG
AGGGCGACCACACGGTGCTGATATATGGCGGCAGGCCATCAGCGGAGCCTGATGTCACACTGCTGACCGCCTTAAAAAAA
CTGCGCCGCAGCCGTCCGCTGGACGGCATCATCTGGGCGCTGACAGAAGAACAGAGCCGCCAGACAGCGCAACTCGACAA
AGGCTGGCGCGAACTGATAAACGGCGGTAAGCGACTCGGTTTTCAGGCCCCACTCTATTTGTGGCAGGTCTGTGACAACG
GTGATTATCAGGCCGGACGCCCCCTGCAAAGCGTCGGCTGCCTGCTGCCGGAACGCTGCACCCCGGAACAACTGACCGCC
ATGCTGGAAGCTCACACCCTGCCGCTGACGGAACAGGGCATGTCGCAACTGCTGGCTGATAACCGCCATGATTTTCTGCT
GCGTCTGGCCCATACCCTTGCAGAGCGGGGTATCGCTCACTGGCAAAGCGTCCTGAAACCGCTGCTGGCAGGCGGCGCAT
TCTCTTCCCTGCGCCTGCGTGGTCTGATGTTCAGCCCGCCGCTTACCGCCGTACCGGAGGCCGCACCTCATGCGTGGCTG
CCGTCACAGGTCTGGGCGGGCGTGACCGGCGATAACGCACGGGGGCGTGCAGTGGGCTTTCCGTGGCTACGCGCCGCGCT
GATGTCCGCTGTCTGCGTGCTGGCAATATGGGGGGCCGGAATGACGACCTCTTTCTTCGCCAACTGCGCTCTTGTTCAGG
AAACCGGTATCCAGACGGCGCGGGCGCTCGATACCCGCCTGCCGCTGGCAGAACAACTGGTGGCGCTGCATACCCTGCAG
GGCGAACTGGAACGCCTGCAATATCGTATCCGCGAAGGTGCGCCGTGGTATCAGCGTTTTGGCCTTGAACGTAACCAACA
ACTGCTCGCCGCCGCCTTTCCCGGCTATGCGCAGGCGGCAAACCGGCTGGTGCGCGACGTGGCCGTTGACCATCTGCAAC
AGCAACTGAACGCCTTTGTCGCCCTGCCGCCCAACAGTCCTCAGCGTACCGCCACCGGTGAACAACGCTATAAGCAGCTT
AAGGCATTGCTGATGACTTCCCGCCCGGAAAAGGCCGACGCTGCCTTTTTCAGTACCACGCTGATGGCAGACAGCCTGCG
CTACGAGAATATCCCGGAAGGTGTGCAGCAGAGCGTGTTTCCGTCACTGCTGACCTTCTGGATGGCGAACCTGCCGGAAC
ACCCGCAGTGGAAAACCTCGCCGCCACCGGAACTGACCGGCGCGGTGCGTAAAATCCTGCTGCGCCAGATTGGTGTGCGT
AATGCCGAAAACACTCTCTACCAGAACGTACTGCAACAGGTGTCCCGCAACTACGCCGATATGACGCTGGCGGACATGAC
CGGGGATACCCTCACCGAATCTCTTTTCAGTACGGAACAGACGGTGCCGGGGATGTTCACCCGTCAGGCGTGGGAAGGAC
AGGTCAGGGAAGCCATCGAGCAGGTGGTGACGGCGCGGCACGAGGAAATCGACTGGGTGCTCAGCGACCGCCAGCAGGAT
ACCTCTGCGGATATATCGCCGGATACGCTGCGTAACCGTCTCACCTCACGCTACTTTACCGACTTCACCGGAAGCTGGCT
GGCGTTTCTCAACAGCATTCGCTGGAAAAAGGAAGACTCGCTCTCCGGCATTCTCGACCAGCTGACACTGATGGCCGATG
CCCGTCAGTCGCCGCTGATTGCGCTGACCGATACCCTCGCCTGGCAGGCGGCGGCAGGCAGGGAAAACCGTGGCCTGTCA
GACTCGCTGGCGAAATCGGCACAGGAACTGTTTAACGGCAAGGAGAAAACGCCGCAGCAATCCCGTGAAGGCGACGTGCC
TGTCGGGCCGCTGGATAAAACCTTCGCTCCGCTGCTGCGGTTGCTGGGCGATAAAGCCGGAGGCGGCGACAGCCAGCTGA
GTCTGCAGACCTACCTCACCCGCGTTACCCGTGTGCGTCTCAAACTGCAACAGGTGACCAACGCCCCCGACCCGCAGGAA
ATGACGCAACAGCTGGCACAGACGGTACTGCAGGGCAAAACCGTTGACCTCACCGACACCCGCGACTACGGACGGTTAAT
CGCCGCCAGTCTGGGCGAGGAATGGAGTGGCTTCGGTCAGGCGCTGTTCGTCCGCCCGGTGGAGCAGTCGTGGCGGCAGG
TGCTGACGCCTGCGGCGGACAGCCTGAACCGCCAGTGGCAGCGGGCGATTGTCAGCCACTGGAATCAGGACTTCGCTGGT
CGCTACCCGTTCAAAGCCTCACAGAACGATGCCTCCCTCCCCCTGCTGGCGCAGTATCTGCGCGATGACGGGCGCATCAA
CCAGTTTATTGCCGCCAACCTTTCCGGCGTTCTGAAACGTGAAGGCCGCTACTGGGTGGCGGACGCCATGAACACGCAGG
GGCTGACGGTCAACCCGGACTTTATCCGCGCCCTGAACCGCCTGCGCGACGTGGCTGACACCGCCTTTGCCAGCGGCGAT
GCCGGAATACATTTTGAACTGCAGGCAAAACCGGCCCGAGACGTGATGAAGACACATCTGGTGATTGACGGGCAGGAACT
GGAGTATTTCAACCAGAAAGAACGCTGGCAGCGTTTCAACTGGCCGGATGAACAGTGGCAACCCGGCGCATCGCTGAGCT
GGACCAGTACGCAGGCGATGGAACGCATACTGGCGGATTACCGGGGGAGCTGGAGCCTTATTCGCCTGCTGGAACAGGCG
CAGGTGACACCGGTGGACAGCAGCACCTTTAAAGTGGTGTGGAAAGCGCAGGACGGCCTGCCGCTGAATTACCTGCTGCG
GGTTGAACAGGGTAAAGGGCCGCTGGCGCTGCTGGAACTGAAAAACTTCCGCCTGCCGGGACAGGTATTTCTGACCGGCA
GGAGTATGAAAGATGCGGAAGAATACGGGGAAGACGCCGATGAGTGA