Detailed information of component protein


Summary

Component ID T6CP016158
Component protein type Vt4A
T6SS ID (Type) T6SS00946 experimental (Type Unknown)
Strain Escherichia coli strain PE117
Replicon chromosome I
Sequence Protein sequence (838 a.a.); Nucleotide sequence (2514 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) -
Coordinate (Strand) 1..2514 (+)
NCBI ID 1062685229
GenBank KX118293
Uniprot ID A0A0C5ENH0_ECOLX
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 9.5e-59 32..347
DUF2345 PF10106.11 2.5e-49 605..745
T6SS_Vgr PF13296.8 1e-32 486..587
Phage_base_V PF04717.14 1.8e-09 399..465
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-837 MDIPTLNFDHSHHKLKIRGLKSPADVLTFTGHEQLSSPFRYDIEFTSTNKAITPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITSFKHLSSSQDEARYEVRLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKNEYPAREQVMQYGEDDLTFVSRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGLFDGETEAVWGLNSAYSVVEKSVSTRDYNYREATAEMTTGQHDATGGDNTTYGEAYHYADNFLQKGDKEAAESGAFYARIRHERYLNEQAILQGQSTSSLLMPGLEIKVQGDDAPAVFRKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPCPVMAGTLPARVTSTVKNDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQLNLGHLVDAGKQQRGEGFELRTDLWGTVRAKKGIFISSDAQDKAQGKVREMAPAMAILDGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLTQAAILLSAPKGVAIASGEHLQLAASKNLIANAGNHADIGVVKNMFIGVGQALSVFVRKAGIKLFANKGAISVQAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEYNVKAGYYGRKPKAKLTPELMAFPVIKSEDFNQSFILLDENTGQPLINWPYELELESGLKMSGITDENGNTELISSDKEEVVNISVFEPDEFLDDEIN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9928 - -


  Protein sequence: 838 a.a.    

>T6CP016158 KX118293:1-2514 [Escherichia coli] [Vt4A]
MDIPTLNFDHSHHKLKIRGLKSPADVLTFTGHEQLSSPFRYDIEFTSTNKAITPESVLMQDGAFSLTAPPVQGMPVQTAL
RTLHGVITSFKHLSSSQDEARYEVRLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKNEYPAREQV
MQYGEDDLTFVSRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGLFDGETEAVWGLNSAYSVVEKSV
STRDYNYREATAEMTTGQHDATGGDNTTYGEAYHYADNFLQKGDKEAAESGAFYARIRHERYLNEQAILQGQSTSSLLMP
GLEIKVQGDDAPAVFRKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPCPVMAGTLPARVTSTVKNDIYAHID
KDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAFEEGNPDRPYIAGVKHDSAHTDHVTIQNY
KRNVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQLNLGHLVDAGKQQRGEGFELRTDLWGTVRAKKGIFISSDAQDKAQ
GKVREMAPAMAILDGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLTQAAILLSAPKGVAIASGEHLQLAASKNL
IANAGNHADIGVVKNMFIGVGQALSVFVRKAGIKLFANKGAISVQAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNG
GGSYIRLDACGIEAGTPGEYNVKAGYYGRKPKAKLTPELMAFPVIKSEDFNQSFILLDENTGQPLINWPYELELESGLKM
SGITDENGNTELISSDKEEVVNISVFEPDEFLDDEIN*

  Nucleotide sequence: 2514 bp    

>T6CP016158 KX118293:1-2514 [Escherichia coli] [Vt4A]
ATGGATATCCCGACTCTGAATTTCGACCACTCACACCACAAGCTCAAAATTCGCGGCCTGAAAAGCCCGGCGGATGTGCT
GACGTTTACGGGCCACGAACAGCTCAGCTCACCCTTCCGCTACGACATTGAGTTTACCAGTACCAATAAAGCAATAACGC
CGGAGTCGGTGCTGATGCAGGACGGCGCATTCAGCCTGACGGCACCGCCCGTGCAGGGAATGCCGGTGCAGACTGCCCTG
CGCACCCTGCACGGCGTCATTACCAGCTTTAAACACCTCTCATCGTCACAGGACGAAGCCCGTTACGAAGTGCGCCTTGA
ACCCCGTATGGCGCTGCTTACCCGCAGCCGCCAGAACGCCATCTACCAGAACCAGACGGTGCCGCAAATCGTCGAAAAAA
TCCTGCGCGAGCGCCATCAAATGCGCGGTCAGGACTTCGTGTTTAACCTCAAAAACGAATACCCGGCCCGTGAACAGGTG
ATGCAGTACGGTGAGGACGACCTGACGTTTGTCTCACGCCTGCTGTCGGAGGTGGGTATCTGGTTCCGCTTTGCCACCGA
CGCCCGGCTGAAAATCGAAGTTGTCGAGTTTTATGACGACCAGAGCGGCTATGAGCGCGGACTGACGCTGCCGCTTCGTC
ATCCTTCAGGGCTGTTTGATGGTGAAACGGAAGCCGTATGGGGGTTGAACAGCGCGTACAGCGTGGTGGAAAAAAGCGTC
TCCACGCGGGACTACAACTACCGTGAAGCCACGGCGGAAATGACCACAGGCCAGCATGACGCCACCGGGGGCGATAACAC
CACCTACGGCGAAGCCTACCACTACGCTGACAACTTCCTGCAAAAAGGCGATAAAGAGGCAGCGGAAAGCGGCGCGTTTT
ATGCCCGTATCCGCCACGAACGCTATTTGAATGAACAGGCCATTCTACAGGGCCAGAGCACCAGTTCACTGCTGATGCCG
GGGCTGGAGATAAAAGTTCAGGGTGACGACGCGCCAGCGGTGTTCCGCAAGGGCGTACTGATTACCGGCGTGACGGCCAG
CGCGGCGCGTGACCGCAGCTATGAACTGACGTTTACCGCCATCCCGTACTCAGAACGCTATGGCTACCGCCCGGCGCTTA
TCCCCTGCCCCGTGATGGCCGGCACGCTCCCGGCACGGGTCACCAGCACGGTGAAGAACGACATCTACGCCCATATCGAC
AAGGACGGGCGCTACCGGGTCAACCTCGACTTTGACCGCGATACGTGGAAACCGGGTTATGAAAGCCTGTGGGTACGCCA
GTCGCGCCCGTATGCGGGAGACACCTACGGCCTGCATCTGCCGCTGCTGGCGGGAACGGAAGTCAGCATCGCGTTTGAGG
AAGGCAACCCGGACCGACCTTATATTGCAGGCGTAAAACACGACTCGGCACACACCGACCATGTGACCATTCAGAACTAC
AAGCGCAACGTACTGCGCACCCCGGCGAACAACAAAATCCGCCTCGATGACGAGCGCGGCAAAGAGCATATCAAGGTCAG
TACCGAGTATGGCGGCAAGAGCCAGCTCAACCTCGGCCATCTGGTGGATGCCGGAAAACAGCAGCGCGGGGAAGGCTTTG
AACTGCGTACCGATCTGTGGGGTACAGTACGGGCAAAGAAAGGCATCTTTATCAGCTCCGACGCGCAGGACAAGGCGCAG
GGTAAGGTGCGCGAAATGGCTCCGGCGATGGCAATACTGGATGGCGCACAGTCACAGATGAAGTCGCTGTCCACTGATGC
GCAGACGGCGAATGCCGATCCGGCAGATCTGTCATCACAGATAGCGCTGTTGCAGCAGAGTGTAAAAGATCTGACGCAGG
CTGCGATCCTGCTAAGCGCACCCAAAGGCGTGGCGATCGCCAGCGGAGAGCACCTGCAACTGGCGGCGTCGAAAAACCTG
ATCGCGAACGCAGGAAATCACGCTGATATCGGTGTCGTGAAGAATATGTTTATCGGCGTGGGTCAGGCGCTGAGCGTGTT
TGTACGCAAAGCGGGTATCAAACTGTTTGCCAATAAAGGGGCGATCTCGGTACAGGCACAGAACGATCTGATGGAACTGT
TGGCGCAGAAATCGATAGAGATCACCAGTACGGAAGATGAGATTAAAATCACCGCGAAGAAGAAAATCACCCTTAACGGC
GGTGGCAGCTACATCCGGCTGGATGCCTGTGGGATAGAGGCCGGTACGCCGGGAGAATATAACGTTAAGGCGGGATATTA
CGGGCGTAAGCCGAAGGCGAAGCTGACGCCGGAGCTGATGGCATTTCCGGTGATTAAGTCTGAGGATTTTAATCAGAGCT
TTATTTTGCTGGATGAAAATACTGGGCAACCACTTATTAATTGGCCCTATGAACTAGAACTGGAAAGTGGGCTAAAAATG
AGTGGGATTACTGATGAAAATGGAAATACAGAACTAATATCCTCTGATAAGGAGGAGGTCGTTAATATTAGTGTGTTTGA
ACCCGATGAGTTTTTAGACGATGAAATTAATTAA