Detailed information of component protein
Summary
| Component ID | T6CP016151 |
| Component protein type | Het2B |
| T6SS ID (Type) | T6SS00943  (Type Unknown) |
| Strain | Escherichia coli strain PE321 |
| Replicon | chromosome I |
| Sequence | Protein sequence (221 a.a.); Nucleotide sequence (663 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | - |
| Coordinate (Strand) | 2071..2733 (+) |
| NCBI ID | - |
| GenBank | KX118290 |
| Uniprot ID | - |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DUF2931 | PF11153.10 | 1.5e-31 | 31..218 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-220 | MEFNIKLTFVFIMCMILNFNVAAKERISLLAPYDEWYFNFFYPDSLPAEVIYVELLDTDGIYYQFRTLASTNASPGSVGQWSEKVLGMSSVFNKAKNPPMSIHFCWDSVIDKKVYETWITLAGDVWKLMLTPYSPPGGGSEKYYLRYLLIGLAPEGKIGVWLEKPDKPNIRLTDKQILIETVSGEKMEMCNGRSAHKHGYSYPESTKNFIKDKQYPYGNW |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.8580 | pos: 23-24. VAA-KE. | MEFNIKLTFVFIMCMILNFNVAA |
Protein sequence: 221 a.a.
>T6CP016151 KX118290:2071-2733 [Escherichia coli] [Het2B]
MEFNIKLTFVFIMCMILNFNVAAKERISLLAPYDEWYFNFFYPDSLPAEVIYVELLDTDGIYYQFRTLASTNASPGSVGQ
WSEKVLGMSSVFNKAKNPPMSIHFCWDSVIDKKVYETWITLAGDVWKLMLTPYSPPGGGSEKYYLRYLLIGLAPEGKIGV
WLEKPDKPNIRLTDKQILIETVSGEKMEMCNGRSAHKHGYSYPESTKNFIKDKQYPYGNW*
MEFNIKLTFVFIMCMILNFNVAAKERISLLAPYDEWYFNFFYPDSLPAEVIYVELLDTDGIYYQFRTLASTNASPGSVGQ
WSEKVLGMSSVFNKAKNPPMSIHFCWDSVIDKKVYETWITLAGDVWKLMLTPYSPPGGGSEKYYLRYLLIGLAPEGKIGV
WLEKPDKPNIRLTDKQILIETVSGEKMEMCNGRSAHKHGYSYPESTKNFIKDKQYPYGNW*
Nucleotide sequence: 663 bp
>T6CP016151 KX118290:2071-2733 [Escherichia coli] [Het2B]
ATGGAATTTAATATAAAGTTAACGTTTGTTTTTATTATGTGTATGATTCTTAATTTTAACGTAGCAGCGAAAGAAAGAAT
ATCGTTGCTTGCACCCTATGATGAGTGGTATTTCAATTTTTTCTACCCTGATTCCCTACCAGCAGAAGTTATCTATGTGG
AATTATTAGATACCGATGGCATCTATTATCAGTTCAGAACGCTGGCCAGTACCAATGCCAGTCCAGGAAGTGTTGGACAA
TGGAGCGAGAAAGTATTAGGGATGTCGTCAGTTTTTAATAAAGCCAAAAATCCCCCTATGTCTATCCATTTTTGCTGGGA
CTCAGTGATTGATAAAAAAGTGTATGAAACCTGGATAACGCTGGCGGGAGACGTCTGGAAATTGATGCTCACACCTTATT
CACCACCAGGCGGTGGCAGTGAAAAGTATTATCTGCGGTATCTGTTAATCGGGCTGGCTCCGGAAGGGAAAATAGGAGTG
TGGCTGGAGAAACCTGACAAGCCCAATATTCGCCTGACGGATAAGCAGATTCTGATAGAAACGGTTTCAGGTGAAAAAAT
GGAAATGTGTAACGGCCGAAGTGCCCATAAACATGGGTATTCTTATCCGGAAAGTACAAAAAACTTTATCAAAGATAAAC
AATATCCCTATGGCAACTGGTAG
ATGGAATTTAATATAAAGTTAACGTTTGTTTTTATTATGTGTATGATTCTTAATTTTAACGTAGCAGCGAAAGAAAGAAT
ATCGTTGCTTGCACCCTATGATGAGTGGTATTTCAATTTTTTCTACCCTGATTCCCTACCAGCAGAAGTTATCTATGTGG
AATTATTAGATACCGATGGCATCTATTATCAGTTCAGAACGCTGGCCAGTACCAATGCCAGTCCAGGAAGTGTTGGACAA
TGGAGCGAGAAAGTATTAGGGATGTCGTCAGTTTTTAATAAAGCCAAAAATCCCCCTATGTCTATCCATTTTTGCTGGGA
CTCAGTGATTGATAAAAAAGTGTATGAAACCTGGATAACGCTGGCGGGAGACGTCTGGAAATTGATGCTCACACCTTATT
CACCACCAGGCGGTGGCAGTGAAAAGTATTATCTGCGGTATCTGTTAATCGGGCTGGCTCCGGAAGGGAAAATAGGAGTG
TGGCTGGAGAAACCTGACAAGCCCAATATTCGCCTGACGGATAAGCAGATTCTGATAGAAACGGTTTCAGGTGAAAAAAT
GGAAATGTGTAACGGCCGAAGTGCCCATAAACATGGGTATTCTTATCCGGAAAGTACAAAAAACTTTATCAAAGATAAAC
AATATCCCTATGGCAACTGGTAG