SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP016055
Component protein type	TssD
T6SS ID (Type)	T6SS00930 (Type i1)
Strain	Escherichia coli SEPT362
Replicon	chromosome
Sequence	Protein sequence (173 a.a.); Nucleotide sequence (519 bp)
Reference	[1] de Pace F, Nakazato G, Pacheco A, et al. The type VI secretion system plays a role in type 1 fimbria expression and pathogenesis of an avian pathogenic Escherichia coli strain. Infect Immun. 2010 Dec;78(12):4990-8. doi: 10.1128/IAI.00531-10. Epub 2010 Sep 20. PMID: 20855516 [2] de Pace F, Boldrin de Paiva J, Nakazato G, et al. Characterization of IcmF of the type VI secretion system in an avian pathogenic Escherichia coli (APEC) strain. Microbiology (Reading). 2011 Oct;157(Pt 10):2954-2962. doi: 10.1099/mic.0.050005-0. Epub 2011 Jul 21. PMID: 21778203

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	A364_RS23060
Coordinate (Strand)	31762..32280 (+)
NCBI ID	447065702
RefSeq	NZ_AOGL01000004
Uniprot ID	A0A1Z3UUT6_ECOLX, A0A0E0Y4V8_ECO1C, L3QI00_ECOLX, U9YRG6_ECOLX, V0YIY8_ECOLX, A7ZHX3_ECO24, A0A0E1SSY5_ECOLX, B6HZU9_ECOSE, A0A0H3PHB8_ECO5C, D6I5D3_ECOLX, E0J653_ECOLW, A0A170UYG5_KLEOX, B7LHE1_ECO55, A0A1X3JLW6_ECOLX, A0A1X3L786_ECOLX, F4VA08_ECOLX, A0A1X3IBM1_ECOLX, F4TA69_ECOLX, A0A1X3LM59_ECOLX, A0A1X3J5C3_ECOLX, E9XQ91_ECOLX, E6BQG9_ECOLX, E1IYR3_ECOLX, E1JBS7_ECOLX, D8E9U8_ECOLX, D3QWN1_ECOCB, D7XRD3_ECOLX, E9TA15_ECOLX, Q7DC35_ECO57, I2UQJ1_ECOLX, I2WRX5_ECOLX, F0JUI5_ESCFE
KEGG ID	ecw:EcE24377A_0243, elw:ECW_m0233, esl:O3K_20405, ecy:ECSE_0232, eok:G2583_0242, eck:EC55989_0235, ecs:ECs0234
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_HCP	PF05638.14	5e-32	8..155

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-172	MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEFLHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTSVEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGTSGADDWRAPLEA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9242	-	-

Protein sequence: 173 a.a.

						>T6CP016055 NZ_AOGL01000004:31762-32280 [Escherichia coli SEPT362] [TssD]
MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEFLHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPL
MYNALASGEMLPTTELHWWRTSVEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
SGADDWRAPLEA*					

Nucleotide sequence: 519 bp

						>T6CP016055 NZ_AOGL01000004:31762-32280 [Escherichia coli SEPT362] [TssD]
ATGCCAACACCGTGTTATATCTCCATCACTGGTCAGACCCAGGGAAATATTACTGCAGGTGCATTTACCGCTGATTCTGT
CGGCAACATCTATGTGCAGGGGCACGAAGACGAGATGCTGGTGCAGGAATTCCTGCATAACGTTACTGTCCCGACCGATC
CTCAGTCCGGTCAGCCTGCGGGTCAGCGTGCTCACAAGCCTTTCATTTTTACCGTGGCGCTGAACAAAGCGGTTCCGCTG
ATGTACAACGCACTGGCCTCCGGCGAAATGCTGCCAACCACTGAACTTCACTGGTGGCGTACCTCTGTGGAAGGCAAACA
GGAGCATTATTTCACTACCCGCCTGACTGATTCGACGATTGTCGATATGAAACTGCATATGCCGCATTGCCAGGACCCGG
CGAAACGCGAGTTCACCCAGTTGCTCGAAGTTTCACTGGCCTACCGCAAAATTGAATGGGAACACGTCAAATCTGGCACC
TCCGGTGCCGACGACTGGCGCGCACCGCTGGAAGCATAA					