SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP016052
Component protein type	TssE
T6SS ID (Type)	T6SS00930 (Type i1)
Strain	Escherichia coli SEPT362
Replicon	chromosome
Sequence	Protein sequence (138 a.a.); Nucleotide sequence (414 bp)
Reference	[1] de Pace F, Nakazato G, Pacheco A, et al. The type VI secretion system plays a role in type 1 fimbria expression and pathogenesis of an avian pathogenic Escherichia coli strain. Infect Immun. 2010 Dec;78(12):4990-8. doi: 10.1128/IAI.00531-10. Epub 2010 Sep 20. PMID: 20855516 [2] de Pace F, Boldrin de Paiva J, Nakazato G, et al. Characterization of IcmF of the type VI secretion system in an avian pathogenic Escherichia coli (APEC) strain. Microbiology (Reading). 2011 Oct;157(Pt 10):2954-2962. doi: 10.1099/mic.0.050005-0. Epub 2011 Jul 21. PMID: 21778203

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	A364_RS23085
Coordinate (Strand)	28360..28773 (-)
NCBI ID	446534397
RefSeq	NZ_AOGL01000004
Uniprot ID	U9YLI4_ECOLX, A0A0L6XVK3_ECOLX, F4TA64_ECOLX
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
GPW_gp25	PF04965.16	2.2e-14	31..114

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-137	MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNILNTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSPLDMTFHITAWVTFNETHEVLEFDMAPNSSQHYRVD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9348	-	-

Protein sequence: 138 a.a.

						>T6CP016052 NZ_AOGL01000004:c28773-28360 [Escherichia coli SEPT362] [TssE]
MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNILNTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCI
ERYEPRISHAEVQAVVSSASSPLDMTFHITAWVTFNETHEVLEFDMAPNSSQHYRVD*					

Nucleotide sequence: 414 bp

						>T6CP016052 NZ_AOGL01000004:c28773-28360 [Escherichia coli SEPT362] [TssE]
ATGATACCGGATCGGGAAAGAACCACAGGTTCTCTTTTTGAGCGTATGGAAGCCTCTTCGGCCAGAAACCGGCAGGGAGG
CAGTATTCATTCGCTCAGGCAATCCATTCGCCAGAACCTACGAAACATTCTTAATACCCGGTCCGGTAGTTGCCGAGGTG
CGCCTGAGCTGGGCATTGATGAACCGGAAGGGGCTGAAAACTTCCGCGAATCCATGAGTCGGGCCATCGAGCAATGTATA
GAACGCTATGAACCCCGGATCTCACACGCTGAAGTGCAGGCAGTGGTTTCATCCGCCTCCAGCCCGCTGGATATGACGTT
TCATATTACAGCCTGGGTGACATTTAACGAAACGCATGAGGTGCTTGAATTTGATATGGCACCGAATAGCAGCCAGCATT
ACCGGGTGGACTGA					