Detailed information of component protein


Summary

Component ID T6CP016049
Component protein type TssJ
T6SS ID (Type) T6SS00930 experimental (Type i1)
Strain Escherichia coli SEPT362
Replicon chromosome
Sequence Protein sequence (175 a.a.); Nucleotide sequence (525 bp)
Reference
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) A364_RS23105
Coordinate (Strand) 23634..24158 (-)
NCBI ID 447002900
RefSeq NZ_AOGL01000004
Uniprot ID U9YRH7_ECOLX, A0A0L6XV99_ECOLX, F4TA60_ECOLX
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS-SciN PF12790.9 1.7e-22 45..164

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-174 MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPSEITVTLLTEPDTNTNAEGESAAVDVQLVYMTDDSKLQAADYDQIASTPLPDVLGKNYIDHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLVHVRQSSIEMKKEDE



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9992 pos: 18-19. LTG-CE. MNRKAFLACVLMCVILTG



  Protein sequence: 175 a.a.    

>T6CP016049 NZ_AOGL01000004:c24158-23634 [Escherichia coli SEPT362] [TssJ]
MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPSEITVTLLTEPDTNTNAEGESAAVDVQLVYMTDDSKL
QAADYDQIASTPLPDVLGKNYIDHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
HVRQSSIEMKKEDE*

  Nucleotide sequence: 525 bp    

>T6CP016049 NZ_AOGL01000004:c24158-23634 [Escherichia coli SEPT362] [TssJ]
ATGAACAGGAAGGCGTTTCTGGCCTGCGTGCTGATGTGCGTCATATTAACCGGCTGCGAAACGGCGAAAAAAATCAGCGA
GGTGATCAAAAACCCGGATATCCAGGTCGGGAGCCTGAAGGAACAACCTTCGGAAATAACCGTCACCCTGCTGACTGAAC
CGGACACCAATACCAATGCGGAGGGTGAATCAGCCGCCGTGGATGTGCAACTGGTGTACATGACTGACGACTCAAAATTG
CAGGCAGCCGACTATGACCAGATAGCCAGTACGCCGCTACCGGATGTGCTGGGTAAAAACTATATCGACCACCAGGATTT
CAACCTGTTGCCGGACACCATCAAAACCCTGCCGCCGGTAAAGCTGGATGAGAAAACGCAGTTTATCGGTGTCGTGGCCT
ATTTCTCCGATGACCAGGCCACTGAATGGAAACAAATCGAGACGGTGGAAGGAACCGGCCACCATTACCGCCTGCTGGTG
CATGTACGTCAAAGCAGCATCGAAATGAAAAAAGAGGATGAATAA