SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP016049
Component protein type	TssJ
T6SS ID (Type)	T6SS00930 (Type i1)
Strain	Escherichia coli SEPT362
Replicon	chromosome
Sequence	Protein sequence (175 a.a.); Nucleotide sequence (525 bp)
Reference	[1] de Pace F, Nakazato G, Pacheco A, et al. The type VI secretion system plays a role in type 1 fimbria expression and pathogenesis of an avian pathogenic Escherichia coli strain. Infect Immun. 2010 Dec;78(12):4990-8. doi: 10.1128/IAI.00531-10. Epub 2010 Sep 20. PMID: 20855516 [2] de Pace F, Boldrin de Paiva J, Nakazato G, et al. Characterization of IcmF of the type VI secretion system in an avian pathogenic Escherichia coli (APEC) strain. Microbiology (Reading). 2011 Oct;157(Pt 10):2954-2962. doi: 10.1099/mic.0.050005-0. Epub 2011 Jul 21. PMID: 21778203

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	A364_RS23105
Coordinate (Strand)	23634..24158 (-)
NCBI ID	447002900
RefSeq	NZ_AOGL01000004
Uniprot ID	U9YRH7_ECOLX, A0A0L6XV99_ECOLX, F4TA60_ECOLX
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	1.7e-22	45..164

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-174	MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPSEITVTLLTEPDTNTNAEGESAAVDVQLVYMTDDSKLQAADYDQIASTPLPDVLGKNYIDHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLVHVRQSSIEMKKEDE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9992	pos: 18-19. LTG-CE.	MNRKAFLACVLMCVILTG

Protein sequence: 175 a.a.

						>T6CP016049 NZ_AOGL01000004:c24158-23634 [Escherichia coli SEPT362] [TssJ]
MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPSEITVTLLTEPDTNTNAEGESAAVDVQLVYMTDDSKL
QAADYDQIASTPLPDVLGKNYIDHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
HVRQSSIEMKKEDE*					

Nucleotide sequence: 525 bp

						>T6CP016049 NZ_AOGL01000004:c24158-23634 [Escherichia coli SEPT362] [TssJ]
ATGAACAGGAAGGCGTTTCTGGCCTGCGTGCTGATGTGCGTCATATTAACCGGCTGCGAAACGGCGAAAAAAATCAGCGA
GGTGATCAAAAACCCGGATATCCAGGTCGGGAGCCTGAAGGAACAACCTTCGGAAATAACCGTCACCCTGCTGACTGAAC
CGGACACCAATACCAATGCGGAGGGTGAATCAGCCGCCGTGGATGTGCAACTGGTGTACATGACTGACGACTCAAAATTG
CAGGCAGCCGACTATGACCAGATAGCCAGTACGCCGCTACCGGATGTGCTGGGTAAAAACTATATCGACCACCAGGATTT
CAACCTGTTGCCGGACACCATCAAAACCCTGCCGCCGGTAAAGCTGGATGAGAAAACGCAGTTTATCGGTGTCGTGGCCT
ATTTCTCCGATGACCAGGCCACTGAATGGAAACAAATCGAGACGGTGGAAGGAACCGGCCACCATTACCGCCTGCTGGTG
CATGTACGTCAAAGCAGCATCGAAATGAAAAAAGAGGATGAATAA					