Detailed information of component protein


Summary

Component ID T6CP016013
Component protein type TssM
T6SS ID (Type) T6SS01290 experimental (Type i1)
Strain Vibrio cholerae DL4215
Replicon chromosome
Sequence Protein sequence (1167 a.a.); Nucleotide sequence (3501 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BL246_RS05985
Coordinate (Strand) 90198..93698 (+)
NCBI ID 446147150
RefSeq NZ_MOLM01000011
Uniprot ID UPI00005F3A83
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 3.2e-77 188..445
IcmF-related PF06761.14 3.5e-71 495..809
IcmF_C PF06744.14 4.8e-24 1039..1145
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-4 VVAA
Transmembrane helix 5-27 LPILLFTTFILLNVAIWWAGPWL
Outside 28-36 EVAGYKPLE
Transmembrane helix 37-59 SIIARVVASSLFTLGCLAVWGIW
Inside 60-437 QWRKLQAFKSEQKREEQLRQDPIKVYEQRQEVELNQVMLNMKQSLNKHNYLYALPWYLVLGLENAGKTSLINRSGQNFVFSSVMRASGQKSENPYSFDWWIGDESVLIDPDGELLTQGNRSEENDGALERRLWLHFVDWLDRTRSRRPLNGIVLALDVAHLATATASERKAYANLLRARLRELMETLSTRLPVYIALTKLDLLHGFEPFFKHYTKSQREEVLGFTFSMDSVDNLDSWLEEFASEYTQFVSRVNGMLPHAVAAPMTLEERNAIYSFTRQISGLKEILQQFFQEALASDQFSTSALVRGAYFTSVYQQGVPTNAFDDAASRRYGLSHAINTAQRAKNSTVYFTQKLFTHIIYPEAGLASDNFRVAKNKRR
Transmembrane helix 438-457 LMGLSFVACSVATLLLAGTW
Outside 458-1166 HRNYLNNVQHADTVLTKVNQYKEQFPTSRSLASQREVLDPLNKIREATLEFGFFRDKPQYISDFGLYQGHTIGPKVEETYLNLLETRFLPLLMADTIVALNQAETDEEKLAVLRVYRMLVDKSGRYQDYVMDYFAKYWQKSFSGQRQIQEELLGHLDYAMRHTDLTAERLNGDKGAEQVMRPYDKVIARAQVELGSMPNDQRVYRNLKLSAQTVLGPSVNLRSLIGPVFDVVFEERVLNSSSLFIPQMLTKRGFDDYFMPQSESVSELALIDSWVLGQSKTAQFSEADKQALREKIRDLYVADYTNTWRAALNEIDVKYFNDINDAVMVLENITSNLEPMQRLLRTLDDNTQLYSALPKDESALKELLKSPKYKVASMIETPFADLNGMLKPVGSKPAYMTEVLASVDELKSYLKSIQDAPDVGMAALDATKARVKLVNADPIYTLKRISSGLPKPLDSMMAKLADESWYVVKQEAIKHLEVRWTEDVYKTFQSKLAGRYPFNPASNKDVALADFEAFFAPNGTLDNFYNQQLKMFIDENISVASDDSAQSIIRKEVLDQIKQAQKIREAFFNRKGILDVSFSVEPLSLSNNKRRSVLNVDGQFLAYSHGPRENVELIWPNTLRDSAVSKVTLIPTQTNMSPRSLQIQGPWAFFRLLDQGDVVSASQTSVDFKFIVDGGEMIYRINAEADANPFTERLFKSFKLSKTLY


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9976 - -


  Protein sequence: 1167 a.a.    

>T6CP016013 NZ_MOLM01000011:90198-93698 [Vibrio cholerae] [TssM]
VVAALPILLFTTFILLNVAIWWAGPWLEVAGYKPLESIIARVVASSLFTLGCLAVWGIWQWRKLQAFKSEQKREEQLRQD
PIKVYEQRQEVELNQVMLNMKQSLNKHNYLYALPWYLVLGLENAGKTSLINRSGQNFVFSSVMRASGQKSENPYSFDWWI
GDESVLIDPDGELLTQGNRSEENDGALERRLWLHFVDWLDRTRSRRPLNGIVLALDVAHLATATASERKAYANLLRARLR
ELMETLSTRLPVYIALTKLDLLHGFEPFFKHYTKSQREEVLGFTFSMDSVDNLDSWLEEFASEYTQFVSRVNGMLPHAVA
APMTLEERNAIYSFTRQISGLKEILQQFFQEALASDQFSTSALVRGAYFTSVYQQGVPTNAFDDAASRRYGLSHAINTAQ
RAKNSTVYFTQKLFTHIIYPEAGLASDNFRVAKNKRRLMGLSFVACSVATLLLAGTWHRNYLNNVQHADTVLTKVNQYKE
QFPTSRSLASQREVLDPLNKIREATLEFGFFRDKPQYISDFGLYQGHTIGPKVEETYLNLLETRFLPLLMADTIVALNQA
ETDEEKLAVLRVYRMLVDKSGRYQDYVMDYFAKYWQKSFSGQRQIQEELLGHLDYAMRHTDLTAERLNGDKGAEQVMRPY
DKVIARAQVELGSMPNDQRVYRNLKLSAQTVLGPSVNLRSLIGPVFDVVFEERVLNSSSLFIPQMLTKRGFDDYFMPQSE
SVSELALIDSWVLGQSKTAQFSEADKQALREKIRDLYVADYTNTWRAALNEIDVKYFNDINDAVMVLENITSNLEPMQRL
LRTLDDNTQLYSALPKDESALKELLKSPKYKVASMIETPFADLNGMLKPVGSKPAYMTEVLASVDELKSYLKSIQDAPDV
GMAALDATKARVKLVNADPIYTLKRISSGLPKPLDSMMAKLADESWYVVKQEAIKHLEVRWTEDVYKTFQSKLAGRYPFN
PASNKDVALADFEAFFAPNGTLDNFYNQQLKMFIDENISVASDDSAQSIIRKEVLDQIKQAQKIREAFFNRKGILDVSFS
VEPLSLSNNKRRSVLNVDGQFLAYSHGPRENVELIWPNTLRDSAVSKVTLIPTQTNMSPRSLQIQGPWAFFRLLDQGDVV
SASQTSVDFKFIVDGGEMIYRINAEADANPFTERLFKSFKLSKTLY*

  Nucleotide sequence: 3501 bp    

>T6CP016013 NZ_MOLM01000011:90198-93698 [Vibrio cholerae] [TssM]
GTGGTGGCAGCACTTCCCATTTTGCTATTCACTACGTTCATTTTGCTGAACGTTGCCATTTGGTGGGCAGGCCCTTGGTT
AGAAGTGGCTGGCTATAAACCGCTGGAGTCGATCATAGCTCGAGTTGTGGCAAGCAGTCTGTTTACTCTCGGCTGTTTAG
CCGTGTGGGGCATTTGGCAGTGGCGTAAGCTGCAAGCTTTCAAATCGGAACAAAAGCGCGAAGAGCAACTGCGCCAAGAT
CCGATCAAGGTGTATGAGCAGCGCCAAGAGGTTGAACTGAATCAGGTGATGCTCAATATGAAGCAGAGCCTAAATAAACA
CAACTACTTGTATGCACTGCCTTGGTACTTGGTTCTAGGTTTAGAAAATGCGGGTAAAACCAGCCTGATCAACCGTTCTG
GTCAGAACTTTGTCTTCTCTTCGGTCATGCGAGCTTCGGGACAAAAAAGCGAAAACCCATACTCGTTTGATTGGTGGATT
GGTGATGAATCTGTCCTGATTGATCCTGATGGTGAGCTGTTAACGCAAGGCAATCGTAGTGAAGAGAATGATGGTGCGTT
AGAGCGCCGCTTATGGTTGCATTTTGTCGATTGGCTCGACCGCACGCGTAGCCGCCGTCCGCTAAACGGCATCGTGTTGG
CGTTGGATGTGGCGCATCTTGCTACCGCCACCGCCTCAGAGCGTAAAGCTTATGCCAATTTGCTGCGTGCTCGTCTGCGT
GAGTTGATGGAAACCTTATCGACTCGTTTGCCGGTGTACATTGCACTGACTAAGCTCGACTTATTGCACGGTTTTGAGCC
TTTCTTTAAGCACTACACCAAGAGTCAGCGCGAAGAGGTGTTGGGTTTTACCTTCTCAATGGACTCGGTAGATAACCTAG
ATTCTTGGTTGGAAGAGTTTGCCAGCGAGTACACTCAATTTGTGTCACGAGTTAATGGCATGCTACCACATGCGGTCGCA
GCGCCAATGACGCTTGAAGAACGTAACGCGATTTACAGCTTCACGCGTCAAATCTCAGGTTTGAAAGAGATTTTGCAACA
GTTTTTCCAAGAGGCTTTGGCGAGCGATCAATTCTCAACGTCAGCGCTGGTGCGTGGTGCTTATTTCACGTCGGTTTATC
AACAAGGTGTTCCCACCAACGCTTTTGATGATGCGGCTTCCCGTCGTTATGGTCTGTCTCATGCGATTAATACGGCGCAG
CGCGCAAAAAACTCCACGGTCTATTTCACGCAAAAATTGTTTACCCACATCATCTATCCTGAAGCAGGTTTAGCGTCGGA
TAATTTCCGGGTTGCAAAAAACAAACGCCGTTTGATGGGGCTCTCTTTTGTCGCATGCTCAGTGGCGACTTTGCTGTTGG
CGGGTACTTGGCATCGTAACTACCTCAATAACGTGCAACACGCCGATACTGTATTAACCAAAGTGAATCAGTATAAAGAG
CAGTTCCCGACCAGCCGTTCACTGGCCTCACAAAGAGAGGTATTGGATCCGTTGAACAAGATCCGTGAGGCGACACTGGA
GTTTGGTTTCTTCCGTGATAAACCTCAGTACATCTCCGATTTTGGTCTCTACCAAGGGCACACCATTGGCCCGAAAGTAG
AAGAGACCTACTTAAACTTACTCGAAACTCGCTTCTTGCCTCTGTTGATGGCGGACACCATTGTTGCGCTCAATCAAGCT
GAGACAGATGAAGAAAAATTAGCGGTGCTGCGTGTGTATCGCATGCTGGTCGATAAAAGTGGTCGTTACCAAGACTATGT
GATGGATTATTTTGCGAAGTATTGGCAAAAATCCTTCTCAGGTCAGCGTCAAATTCAGGAAGAGCTGCTCGGCCACTTAG
ATTACGCGATGCGTCATACGGACCTCACTGCAGAGCGTCTCAACGGTGATAAAGGGGCGGAACAGGTGATGCGCCCTTAC
GATAAAGTGATTGCTCGTGCTCAGGTTGAACTGGGTTCAATGCCAAACGACCAGCGCGTCTATCGTAACTTGAAGCTGAG
CGCACAAACGGTGCTTGGTCCTTCGGTTAACTTGCGTAGCTTGATTGGGCCGGTGTTCGATGTGGTGTTTGAAGAGCGCG
TTCTTAACAGTAGCAGCCTGTTTATTCCTCAAATGCTCACCAAGCGTGGTTTTGATGATTACTTTATGCCGCAATCTGAA
TCGGTCTCTGAGTTGGCACTGATTGACAGTTGGGTTTTGGGACAGAGCAAAACGGCGCAGTTTAGTGAAGCAGACAAGCA
GGCACTGCGCGAAAAAATCCGCGATCTTTATGTGGCGGATTACACCAACACATGGCGCGCGGCACTCAATGAAATTGATG
TGAAGTATTTCAATGACATCAATGATGCGGTCATGGTTTTGGAAAATATCACCAGCAACCTTGAGCCGATGCAGCGTCTA
TTGCGCACATTAGATGACAATACCCAACTGTACAGTGCGTTACCTAAAGATGAATCGGCGTTAAAAGAGCTGCTGAAAAG
TCCGAAATACAAAGTGGCTTCGATGATTGAAACTCCATTTGCTGATTTGAACGGTATGCTCAAGCCGGTAGGCAGCAAAC
CAGCGTACATGACGGAAGTTTTGGCTTCGGTGGATGAGCTGAAAAGCTACTTGAAATCGATTCAAGATGCTCCCGATGTT
GGTATGGCGGCGCTCGATGCGACCAAAGCGCGGGTCAAACTGGTTAATGCTGACCCTATCTATACCTTAAAACGTATCTC
GTCAGGACTGCCGAAGCCGCTGGATAGCATGATGGCAAAGCTTGCAGATGAGAGCTGGTATGTGGTCAAGCAAGAAGCGA
TCAAGCATCTTGAAGTACGCTGGACGGAGGATGTGTATAAAACCTTCCAAAGCAAGCTGGCTGGGCGTTATCCATTCAAC
CCCGCATCAAATAAAGATGTGGCCTTGGCCGATTTTGAAGCCTTCTTTGCGCCGAATGGCACGTTAGACAATTTCTACAA
TCAGCAACTGAAGATGTTTATTGATGAGAACATCAGCGTCGCGTCGGATGACTCAGCGCAGTCGATCATTCGTAAAGAAG
TGCTGGATCAGATTAAGCAAGCGCAGAAAATTCGTGAGGCTTTCTTCAACCGTAAAGGGATTTTGGATGTCAGCTTCTCG
GTTGAGCCGCTCAGTTTGAGTAACAACAAGCGTCGTAGTGTGCTGAATGTGGATGGTCAGTTCTTAGCATACAGCCATGG
CCCGCGTGAAAATGTCGAGCTGATTTGGCCTAACACGCTGCGTGATTCTGCGGTGTCTAAGGTGACCTTGATTCCAACCC
AAACCAATATGTCACCGCGCAGTTTGCAAATCCAAGGACCGTGGGCATTCTTCCGCTTATTGGATCAAGGTGATGTGGTT
TCGGCGAGTCAAACGTCGGTTGATTTCAAATTTATCGTCGATGGCGGTGAGATGATCTACCGTATTAACGCAGAGGCCGA
TGCCAACCCATTTACCGAACGCCTGTTCAAGTCGTTTAAACTGTCTAAAACACTCTATTAA