Detailed information of component protein
Summary
| Component ID | T6CP016008 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS01290  (Type i1) |
| Strain | Vibrio cholerae DL4215 |
| Replicon | chromosome |
| Sequence | Protein sequence (159 a.a.); Nucleotide sequence (477 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | BL246_RS05950 |
| Coordinate (Strand) | 81245..81721 (+) |
| NCBI ID | 446968055 |
| RefSeq | NZ_MOLM01000011 |
| Uniprot ID | Q9KN52_VIBCH, A0A0X1L1T9_VIBCO, A0A0H3AEY5_VIBC3, D7HAU7_VIBCL, A0A0K9UWF9_VIBCL, A0A0H3Q3J5_VIBCL, C3LUD7_VIBCM, A0A2A1YU60_VIBCL |
| KEGG ID | vcz:VAB027_916, vcm:VCM66_A0111, vco:VC0395_0025, vcq:EN18_04290, vch:VCA0113, vcx:VAA049_1795 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 4.8e-29 | 30..145 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-158 | VNKVLWMLFALTVLTACSSSDKYEPAKEPTKVTFSLVSDEGVNPNIWGEASPIEIQVFELKDDSMFMSSDYDQIKTDYKKALRSNFVKNYDYVLTPGQFKFVNAFEVDEETNYIGVMAHFAEPELSEWKKAVKVINKGREYHLLMLFKDYDVKLDRVE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9993 | pos: 16-17. LTA-CS. | VNKVLWMLFALTVLTA |
Protein sequence: 159 a.a.
>T6CP016008 NZ_MOLM01000011:81245-81721 [Vibrio cholerae] [TssJ]
VNKVLWMLFALTVLTACSSSDKYEPAKEPTKVTFSLVSDEGVNPNIWGEASPIEIQVFELKDDSMFMSSDYDQIKTDYKK
ALRSNFVKNYDYVLTPGQFKFVNAFEVDEETNYIGVMAHFAEPELSEWKKAVKVINKGREYHLLMLFKDYDVKLDRVE*
VNKVLWMLFALTVLTACSSSDKYEPAKEPTKVTFSLVSDEGVNPNIWGEASPIEIQVFELKDDSMFMSSDYDQIKTDYKK
ALRSNFVKNYDYVLTPGQFKFVNAFEVDEETNYIGVMAHFAEPELSEWKKAVKVINKGREYHLLMLFKDYDVKLDRVE*
Nucleotide sequence: 477 bp
>T6CP016008 NZ_MOLM01000011:81245-81721 [Vibrio cholerae] [TssJ]
GTGAACAAAGTGCTGTGGATGCTGTTTGCCTTGACGGTGCTGACCGCGTGCAGTTCGTCGGATAAGTACGAGCCTGCGAA
AGAGCCGACCAAGGTGACTTTTAGCTTAGTCAGCGATGAAGGGGTGAACCCGAACATCTGGGGTGAAGCGTCACCGATAG
AAATTCAAGTTTTTGAGCTAAAGGATGACTCGATGTTTATGTCGTCCGACTACGACCAGATCAAAACCGATTATAAAAAA
GCGCTGCGCAGTAACTTTGTTAAAAACTACGACTATGTACTGACTCCAGGGCAGTTCAAATTCGTCAACGCTTTCGAAGT
CGATGAAGAAACTAACTACATCGGTGTGATGGCTCATTTTGCCGAGCCGGAACTTAGCGAATGGAAGAAAGCGGTCAAGG
TGATCAATAAAGGGCGAGAGTATCACTTGCTGATGCTGTTTAAAGACTACGATGTGAAATTGGATAGGGTGGAATAA
GTGAACAAAGTGCTGTGGATGCTGTTTGCCTTGACGGTGCTGACCGCGTGCAGTTCGTCGGATAAGTACGAGCCTGCGAA
AGAGCCGACCAAGGTGACTTTTAGCTTAGTCAGCGATGAAGGGGTGAACCCGAACATCTGGGGTGAAGCGTCACCGATAG
AAATTCAAGTTTTTGAGCTAAAGGATGACTCGATGTTTATGTCGTCCGACTACGACCAGATCAAAACCGATTATAAAAAA
GCGCTGCGCAGTAACTTTGTTAAAAACTACGACTATGTACTGACTCCAGGGCAGTTCAAATTCGTCAACGCTTTCGAAGT
CGATGAAGAAACTAACTACATCGGTGTGATGGCTCATTTTGCCGAGCCGGAACTTAGCGAATGGAAGAAAGCGGTCAAGG
TGATCAATAAAGGGCGAGAGTATCACTTGCTGATGCTGTTTAAAGACTACGATGTGAAATTGGATAGGGTGGAATAA