Detailed information of component protein
Summary
| Component ID | T6CP015959 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS00945  (Type i1) |
| Strain | Vibrio proteolyticus NBRC 13287 |
| Replicon | chromosome II |
| Sequence | Protein sequence (264 a.a.); Nucleotide sequence (792 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | VPR01S_RS08910 |
| Coordinate (Strand) | 45884..46675 (+) |
| NCBI ID | 750620622 |
| RefSeq | NZ_BATJ01000006 |
| Uniprot ID | UPI000593C3BE |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 1.4e-49 | 47..247 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-230 | MSYADDDVTVVLFQPEPGKPMEVMPAPDLSRNIAVDDLDISTMGVNPLVDQFSWLIASLSCMDSIPWLDDPMPFREQVAREIRKGERKLNEMEIDRATILVVRYCLCAAVDEAVLAQEWGTNSRWSQNSLLAEFHNETSGGDKFFVILERLKAEPRKYRYVIEFLYLLLQLGFKGKFGREERGNEQLAEIGNTIYRLIRDDRLAEQEKVSLVNLKAKYLKKPLKRVISPK |
| Transmembrane helix | 231-253 | MILGVTALVFAAMYAASYLLIDV |
| Outside | 254-263 | KYQQLLGVYQ |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9965 | - | - |
Protein sequence: 264 a.a.
>T6CP015959 NZ_BATJ01000006:45884-46675 [Vibrio proteolyticus NBRC 13287] [TssL]
MSYADDDVTVVLFQPEPGKPMEVMPAPDLSRNIAVDDLDISTMGVNPLVDQFSWLIASLSCMDSIPWLDDPMPFREQVAR
EIRKGERKLNEMEIDRATILVVRYCLCAAVDEAVLAQEWGTNSRWSQNSLLAEFHNETSGGDKFFVILERLKAEPRKYRY
VIEFLYLLLQLGFKGKFGREERGNEQLAEIGNTIYRLIRDDRLAEQEKVSLVNLKAKYLKKPLKRVISPKMILGVTALVF
AAMYAASYLLIDVKYQQLLGVYQ*
MSYADDDVTVVLFQPEPGKPMEVMPAPDLSRNIAVDDLDISTMGVNPLVDQFSWLIASLSCMDSIPWLDDPMPFREQVAR
EIRKGERKLNEMEIDRATILVVRYCLCAAVDEAVLAQEWGTNSRWSQNSLLAEFHNETSGGDKFFVILERLKAEPRKYRY
VIEFLYLLLQLGFKGKFGREERGNEQLAEIGNTIYRLIRDDRLAEQEKVSLVNLKAKYLKKPLKRVISPKMILGVTALVF
AAMYAASYLLIDVKYQQLLGVYQ*
Nucleotide sequence: 792 bp
>T6CP015959 NZ_BATJ01000006:45884-46675 [Vibrio proteolyticus NBRC 13287] [TssL]
ATGAGTTACGCAGATGATGATGTAACCGTTGTTCTCTTTCAGCCTGAGCCCGGCAAGCCTATGGAGGTTATGCCGGCGCC
GGATCTGTCGCGTAATATTGCGGTGGATGATCTGGATATTTCCACCATGGGGGTTAACCCCTTGGTGGATCAGTTTTCGT
GGCTGATTGCGAGTTTATCCTGTATGGACTCGATTCCATGGCTGGACGATCCAATGCCATTTCGCGAGCAGGTAGCACGA
GAAATTCGCAAAGGTGAACGCAAGCTCAATGAGATGGAAATCGACCGGGCGACCATTCTGGTGGTGCGTTACTGTTTATG
TGCTGCGGTCGACGAAGCGGTACTGGCGCAGGAGTGGGGCACCAACAGCCGTTGGAGTCAGAACAGCTTGTTGGCCGAGT
TTCACAATGAGACATCGGGCGGTGATAAGTTTTTCGTTATCCTTGAGCGCCTGAAAGCAGAACCAAGAAAGTATCGCTAT
GTAATTGAGTTTCTGTACTTGCTGCTACAGCTTGGTTTTAAAGGTAAATTTGGCCGTGAAGAGCGTGGTAACGAGCAACT
GGCGGAAATTGGCAATACAATTTATCGTCTGATTCGGGATGACCGTCTTGCGGAACAAGAGAAAGTTTCGTTGGTCAATC
TTAAGGCGAAATACCTTAAGAAGCCGCTCAAGCGCGTCATCTCACCGAAAATGATACTCGGTGTGACCGCCCTAGTTTTT
GCTGCCATGTACGCGGCGTCTTATCTGCTGATCGATGTTAAGTACCAGCAACTTCTGGGTGTGTACCAATAA
ATGAGTTACGCAGATGATGATGTAACCGTTGTTCTCTTTCAGCCTGAGCCCGGCAAGCCTATGGAGGTTATGCCGGCGCC
GGATCTGTCGCGTAATATTGCGGTGGATGATCTGGATATTTCCACCATGGGGGTTAACCCCTTGGTGGATCAGTTTTCGT
GGCTGATTGCGAGTTTATCCTGTATGGACTCGATTCCATGGCTGGACGATCCAATGCCATTTCGCGAGCAGGTAGCACGA
GAAATTCGCAAAGGTGAACGCAAGCTCAATGAGATGGAAATCGACCGGGCGACCATTCTGGTGGTGCGTTACTGTTTATG
TGCTGCGGTCGACGAAGCGGTACTGGCGCAGGAGTGGGGCACCAACAGCCGTTGGAGTCAGAACAGCTTGTTGGCCGAGT
TTCACAATGAGACATCGGGCGGTGATAAGTTTTTCGTTATCCTTGAGCGCCTGAAAGCAGAACCAAGAAAGTATCGCTAT
GTAATTGAGTTTCTGTACTTGCTGCTACAGCTTGGTTTTAAAGGTAAATTTGGCCGTGAAGAGCGTGGTAACGAGCAACT
GGCGGAAATTGGCAATACAATTTATCGTCTGATTCGGGATGACCGTCTTGCGGAACAAGAGAAAGTTTCGTTGGTCAATC
TTAAGGCGAAATACCTTAAGAAGCCGCTCAAGCGCGTCATCTCACCGAAAATGATACTCGGTGTGACCGCCCTAGTTTTT
GCTGCCATGTACGCGGCGTCTTATCTGCTGATCGATGTTAAGTACCAGCAACTTCTGGGTGTGTACCAATAA