Detailed information of component protein
Summary
| Component ID | T6CP015957 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS00945  (Type i1) |
| Strain | Vibrio proteolyticus NBRC 13287 |
| Replicon | chromosome II |
| Sequence | Protein sequence (167 a.a.); Nucleotide sequence (501 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | VPR01S_RS08900 |
| Coordinate (Strand) | 44050..44550 (+) |
| NCBI ID | 545468489 |
| RefSeq | NZ_BATJ01000006 |
| Uniprot ID | UPI0003986934 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 9.5e-30 | 34..149 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-166 | MRKLTVALTLFLLSGCAMWEQFKESSGITPETSSIELVIEASDVLNVRDGGQPSPVIMRVHELTSPVLFRSLDFFALFENDKAALGDEYIKRYEYQIKPGEKIHELIELDPTTRALGFSVAFRDINGSSWRKVEAIEEKTEYFLRLKLEGSEMISDTTKGIEQTYF |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9980 | pos: 15-16. LSG-CA. | MRKLTVALTLFLLSG |
Protein sequence: 167 a.a.
>T6CP015957 NZ_BATJ01000006:44050-44550 [Vibrio proteolyticus NBRC 13287] [TssJ]
MRKLTVALTLFLLSGCAMWEQFKESSGITPETSSIELVIEASDVLNVRDGGQPSPVIMRVHELTSPVLFRSLDFFALFEN
DKAALGDEYIKRYEYQIKPGEKIHELIELDPTTRALGFSVAFRDINGSSWRKVEAIEEKTEYFLRLKLEGSEMISDTTKG
IEQTYF*
MRKLTVALTLFLLSGCAMWEQFKESSGITPETSSIELVIEASDVLNVRDGGQPSPVIMRVHELTSPVLFRSLDFFALFEN
DKAALGDEYIKRYEYQIKPGEKIHELIELDPTTRALGFSVAFRDINGSSWRKVEAIEEKTEYFLRLKLEGSEMISDTTKG
IEQTYF*
Nucleotide sequence: 501 bp
>T6CP015957 NZ_BATJ01000006:44050-44550 [Vibrio proteolyticus NBRC 13287] [TssJ]
ATGCGTAAGTTGACGGTCGCCCTGACACTGTTTTTGTTATCGGGCTGCGCGATGTGGGAACAGTTTAAAGAATCTTCTGG
TATTACCCCGGAAACCTCCTCGATTGAACTGGTGATCGAAGCGTCGGACGTGTTGAACGTTCGAGATGGCGGCCAGCCAT
CGCCGGTCATTATGCGAGTACATGAACTGACTTCGCCGGTATTGTTTCGGAGTTTGGATTTCTTTGCGTTGTTTGAAAAC
GACAAGGCGGCACTGGGTGATGAATACATCAAACGTTATGAGTATCAGATAAAGCCAGGCGAAAAAATCCATGAGCTGAT
CGAGCTGGACCCTACCACTCGAGCGCTGGGTTTCTCGGTCGCGTTTCGTGACATTAATGGCTCTTCATGGCGCAAAGTGG
AAGCTATCGAAGAGAAAACAGAATACTTCCTGAGACTGAAACTGGAAGGTAGCGAAATGATCTCTGATACCACGAAAGGT
ATCGAGCAGACTTATTTTTAA
ATGCGTAAGTTGACGGTCGCCCTGACACTGTTTTTGTTATCGGGCTGCGCGATGTGGGAACAGTTTAAAGAATCTTCTGG
TATTACCCCGGAAACCTCCTCGATTGAACTGGTGATCGAAGCGTCGGACGTGTTGAACGTTCGAGATGGCGGCCAGCCAT
CGCCGGTCATTATGCGAGTACATGAACTGACTTCGCCGGTATTGTTTCGGAGTTTGGATTTCTTTGCGTTGTTTGAAAAC
GACAAGGCGGCACTGGGTGATGAATACATCAAACGTTATGAGTATCAGATAAAGCCAGGCGAAAAAATCCATGAGCTGAT
CGAGCTGGACCCTACCACTCGAGCGCTGGGTTTCTCGGTCGCGTTTCGTGACATTAATGGCTCTTCATGGCGCAAAGTGG
AAGCTATCGAAGAGAAAACAGAATACTTCCTGAGACTGAAACTGGAAGGTAGCGAAATGATCTCTGATACCACGAAAGGT
ATCGAGCAGACTTATTTTTAA