Detailed information of component protein
Summary
| Component ID | T6CP015939 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS01282  (Type i3) |
| Strain | Kosakonia oryzae strain KO348 |
| Replicon | chromosome |
| Sequence | Protein sequence (169 a.a.); Nucleotide sequence (507 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | UG97_RS09210 |
| Coordinate (Strand) | 192526..193032 (-) |
| NCBI ID | 780193720 |
| RefSeq | NZ_JZLI01000006 |
| Uniprot ID | UPI0005ED49B7 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 1.2e-27 | 35..138 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-11 | MNNNSVVRQIS |
| Transmembrane helix | 12-34 | LVFFFVLATLASGCGSSSHSVPT |
| Outside | 35-168 | SYNLQFRAHPQINESAPLKVRVLLLKSDATFMSADFWSLQNNADSVLGANLLNSDEFFLMPGQLSKTLSGKSAPDARYIGVMAEYQALDGKKWRMSLPLPVQGETHFYEFWKSSSDELEANIFLDVNGIRVVSK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9957 | pos: 24-25. ASG-CG. | MNNNSVVRQISLVFFFVLATLASG |
Protein sequence: 169 a.a.
>T6CP015939 NZ_JZLI01000006:c193032-192526 [Kosakonia oryzae] [TssJ]
MNNNSVVRQISLVFFFVLATLASGCGSSSHSVPTSYNLQFRAHPQINESAPLKVRVLLLKSDATFMSADFWSLQNNADSV
LGANLLNSDEFFLMPGQLSKTLSGKSAPDARYIGVMAEYQALDGKKWRMSLPLPVQGETHFYEFWKSSSDELEANIFLDV
NGIRVVSK*
MNNNSVVRQISLVFFFVLATLASGCGSSSHSVPTSYNLQFRAHPQINESAPLKVRVLLLKSDATFMSADFWSLQNNADSV
LGANLLNSDEFFLMPGQLSKTLSGKSAPDARYIGVMAEYQALDGKKWRMSLPLPVQGETHFYEFWKSSSDELEANIFLDV
NGIRVVSK*
Nucleotide sequence: 507 bp
>T6CP015939 NZ_JZLI01000006:c193032-192526 [Kosakonia oryzae] [TssJ]
ATGAATAATAATTCGGTTGTACGCCAGATTAGCCTGGTGTTTTTCTTTGTGCTGGCCACCCTGGCCAGCGGTTGTGGCTC
CTCTTCACACAGCGTACCTACCAGCTATAACCTTCAGTTCAGGGCTCATCCGCAAATCAATGAGTCCGCTCCCCTTAAAG
TCAGGGTGTTGCTGTTGAAATCGGACGCGACATTTATGTCCGCCGATTTCTGGTCGCTGCAAAACAACGCAGATAGCGTA
CTTGGCGCCAACCTGCTGAACAGTGATGAATTTTTCCTGATGCCCGGCCAGTTGTCGAAAACGCTGAGCGGTAAAAGCGC
GCCCGACGCGCGTTACATCGGCGTGATGGCGGAGTATCAGGCGCTGGATGGCAAAAAATGGCGGATGTCCTTACCGCTCC
CGGTGCAGGGAGAGACGCACTTCTACGAATTCTGGAAATCGTCGTCAGACGAACTGGAAGCCAACATCTTCCTCGACGTC
AATGGCATCCGTGTGGTTTCCAAATAG
ATGAATAATAATTCGGTTGTACGCCAGATTAGCCTGGTGTTTTTCTTTGTGCTGGCCACCCTGGCCAGCGGTTGTGGCTC
CTCTTCACACAGCGTACCTACCAGCTATAACCTTCAGTTCAGGGCTCATCCGCAAATCAATGAGTCCGCTCCCCTTAAAG
TCAGGGTGTTGCTGTTGAAATCGGACGCGACATTTATGTCCGCCGATTTCTGGTCGCTGCAAAACAACGCAGATAGCGTA
CTTGGCGCCAACCTGCTGAACAGTGATGAATTTTTCCTGATGCCCGGCCAGTTGTCGAAAACGCTGAGCGGTAAAAGCGC
GCCCGACGCGCGTTACATCGGCGTGATGGCGGAGTATCAGGCGCTGGATGGCAAAAAATGGCGGATGTCCTTACCGCTCC
CGGTGCAGGGAGAGACGCACTTCTACGAATTCTGGAAATCGTCGTCAGACGAACTGGAAGCCAACATCTTCCTCGACGTC
AATGGCATCCGTGTGGTTTCCAAATAG