Detailed information of component protein


Summary

Component ID T6CP015939
Component protein type TssJ
T6SS ID (Type) T6SS01282 experimental (Type i3)
Strain Kosakonia oryzae strain KO348
Replicon chromosome
Sequence Protein sequence (169 a.a.); Nucleotide sequence (507 bp)
Reference
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) UG97_RS09210
Coordinate (Strand) 192526..193032 (-)
NCBI ID 780193720
RefSeq NZ_JZLI01000006
Uniprot ID UPI0005ED49B7
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS-SciN PF12790.9 1.2e-27 35..138

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-11 MNNNSVVRQIS
Transmembrane helix 12-34 LVFFFVLATLASGCGSSSHSVPT
Outside 35-168 SYNLQFRAHPQINESAPLKVRVLLLKSDATFMSADFWSLQNNADSVLGANLLNSDEFFLMPGQLSKTLSGKSAPDARYIGVMAEYQALDGKKWRMSLPLPVQGETHFYEFWKSSSDELEANIFLDVNGIRVVSK



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9957 pos: 24-25. ASG-CG. MNNNSVVRQISLVFFFVLATLASG



  Protein sequence: 169 a.a.    

>T6CP015939 NZ_JZLI01000006:c193032-192526 [Kosakonia oryzae] [TssJ]
MNNNSVVRQISLVFFFVLATLASGCGSSSHSVPTSYNLQFRAHPQINESAPLKVRVLLLKSDATFMSADFWSLQNNADSV
LGANLLNSDEFFLMPGQLSKTLSGKSAPDARYIGVMAEYQALDGKKWRMSLPLPVQGETHFYEFWKSSSDELEANIFLDV
NGIRVVSK*

  Nucleotide sequence: 507 bp    

>T6CP015939 NZ_JZLI01000006:c193032-192526 [Kosakonia oryzae] [TssJ]
ATGAATAATAATTCGGTTGTACGCCAGATTAGCCTGGTGTTTTTCTTTGTGCTGGCCACCCTGGCCAGCGGTTGTGGCTC
CTCTTCACACAGCGTACCTACCAGCTATAACCTTCAGTTCAGGGCTCATCCGCAAATCAATGAGTCCGCTCCCCTTAAAG
TCAGGGTGTTGCTGTTGAAATCGGACGCGACATTTATGTCCGCCGATTTCTGGTCGCTGCAAAACAACGCAGATAGCGTA
CTTGGCGCCAACCTGCTGAACAGTGATGAATTTTTCCTGATGCCCGGCCAGTTGTCGAAAACGCTGAGCGGTAAAAGCGC
GCCCGACGCGCGTTACATCGGCGTGATGGCGGAGTATCAGGCGCTGGATGGCAAAAAATGGCGGATGTCCTTACCGCTCC
CGGTGCAGGGAGAGACGCACTTCTACGAATTCTGGAAATCGTCGTCAGACGAACTGGAAGCCAACATCTTCCTCGACGTC
AATGGCATCCGTGTGGTTTCCAAATAG