SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015921
Component protein type	TssJ
T6SS ID (Type)	T6SS01281 (Type i1)
Strain	Vibrio coralliilyticus OCN008
Replicon	chromosome II
Sequence	Protein sequence (167 a.a.); Nucleotide sequence (501 bp)
Reference	[1] Guillemette R, Ushijima B, Jalan M, et al. Insight into the resilience and susceptibility of marine bacteria to T6SS attack by Vibrio cholerae and Vibrio coralliilyticus. PLoS One. 2020 Jan 28;15(1):e0227864. doi: 10.1371/journal.pone.0227864. eCollection 2020. PMID: 31990915

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	G3U99_RS23895
Coordinate (Strand)	1653973..1654473 (+)
NCBI ID	WP_019277701.1
RefSeq	NZ_CP048694
Uniprot ID	UPI0002EA5115
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	5.3e-31	34..149

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-166	MRKLITLFLFLSLTGCSMWDQFKESSGITPETSSIELIIEASPLLNMREGGQPSPVILRIHELTSPVLFRNLDFFALFENDKASLGDEYIKRYEYQLKPGEKIHEVLTLDPGTRALGFSAAFRDINGSSWRKVELIEEQSEYFLKLKLEGSELSFNSTRGIEQTYF

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9984	pos: 15-16. LTG-CS.	MRKLITLFLFLSLTG

Protein sequence: 167 a.a.

						>T6CP015921 NZ_CP048694:1653973-1654473 [Vibrio coralliilyticus OCN008] [TssJ]
MRKLITLFLFLSLTGCSMWDQFKESSGITPETSSIELIIEASPLLNMREGGQPSPVILRIHELTSPVLFRNLDFFALFEN
DKASLGDEYIKRYEYQLKPGEKIHEVLTLDPGTRALGFSAAFRDINGSSWRKVELIEEQSEYFLKLKLEGSELSFNSTRG
IEQTYF*					

Nucleotide sequence: 501 bp

						>T6CP015921 NZ_CP048694:1653973-1654473 [Vibrio coralliilyticus OCN008] [TssJ]
ATGCGTAAACTAATCACTCTTTTCTTATTCCTGTCTTTAACTGGTTGTTCTATGTGGGACCAGTTCAAGGAATCAAGTGG
TATCACACCTGAAACTTCCTCTATCGAGCTGATCATTGAGGCATCTCCTTTGCTTAACATGCGGGAGGGCGGACAGCCGT
CACCTGTGATATTGAGAATTCATGAATTGACTTCGCCAGTCTTGTTTCGAAATTTAGACTTCTTTGCTTTGTTTGAGAAT
GACAAAGCCTCTCTTGGTGATGAGTACATTAAGCGCTATGAATATCAGTTGAAGCCGGGTGAGAAAATTCATGAGGTGCT
GACTCTTGACCCTGGAACTCGTGCATTAGGTTTTTCTGCGGCGTTTCGCGATATCAATGGTTCCTCTTGGCGAAAAGTTG
AATTAATTGAAGAGCAGTCTGAGTACTTTTTAAAACTTAAGCTGGAGGGAAGCGAACTGTCGTTTAACTCTACGCGTGGA
ATCGAGCAAACTTACTTTTAA					