Detailed information of component protein


Summary

Component ID T6CP015918
Component protein type TssM
T6SS ID (Type) T6SS01281 experimental (Type i1)
Strain Vibrio coralliilyticus OCN008
Replicon chromosome II
Sequence Protein sequence (1132 a.a.); Nucleotide sequence (3396 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) G3U99_RS23875
Coordinate (Strand) 1646746..1650141 (-)
NCBI ID WP_165579909.1
RefSeq NZ_CP048694
Uniprot ID UPI0010C391E4
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 3.3e-46 154..407
IcmF-related PF06761.14 4.2e-41 485..754
IcmF_C PF06744.14 4.6e-17 1004..1107
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-8 MLKNIIRH
Transmembrane helix 9-31 PIFISTLLALVLIGGLTAAYFLY
Outside 32-40 PETVSTNIL
Transmembrane helix 41-60 YIGIAAIALVLCAYQLVLFF
Inside 61-400 KKRKQNRDQQLETEEEALSLVLRPLLVGSKKKPIYLMIGNKAAGKKQFLTSSNAIKSIDTSRTKKNDFFEWYESDEAVYLKPDQRLVFQEVSAADSLLWDTMVAEVIRSRPRKPFAGCLFFTDLEFLIISEEEQIDYALTALIDRITSICDQTNTALPVYMVVSKLDKLQGFKEYVQLSPLKHNVEYLSIALKDAKGVLVDYYRDSFNNLVKVIEKNALDSTSQSNDTKEKQAILSFPKQLEICQKEVEYVVQRLSEVNRGAYFIDLRELFFSSCLQGGRKYNLLAKSCSTYFNLPIIASEHTQLSETPYFTRFLVESQILPEADFAGENKTYLKRILRQ
Transmembrane helix 401-423 SRLAMVASVVFLAGGAYLLGTTL
Outside 424-1131 DSNLRVIYQLLNIETGDQAADHVRLLDDKLAQAITQIEPVYDAWVEGSEALDEEIVSLNVSRLEPATKMAYQTLLQSIQKELIPVIEQTYRVQLTKYQDKPIQALPVLKGYLMLKEPSKRDLPFMTTQTQSTLSKVIGDSEQVASAMKYLNAYFRTDFAPVNINMDIVRATRRNLLAQSNVDLVYAQLINQANSIDLGTLNIERAVGFDFNNVFNDQIDQELLDISKVYTATGFSTFYRPRVDLMSKDVITNNWVLGLSKNVTPSKEEHEAFKDQIRKKYTDDYISYWRNALSELKVKKYNSIGELTNAIDLVSGPSSPLTTVLKQVYSNTHFSPTGEKELLVNKLPTAATEAIDKLADSAEEFVQPDYLLMQRVEQAFFQLNRLQINETPNSPTPWDEIVTALSRLRTYMKDIADSPDPQMASLLAARSRMQSSEADPIIRLKQIAQKSPEPVRSWLLGMVQQSWSVMIAESAKGVQTQWYSEVYTKYRDLALNKYPFDLNAEEEIALEDFELLFSTGGIIDTFVQKNLASFYDTNLWTPKRVDGETMPLTPELLVQLKNYNVIRDTLINKSTNRVSIPFTTKILDLDSSAIRATVKVADSQMSYYHGPSRIRELEWPPKSGDFNVSITIQDVTDEGKQYVLSENGQWAIYRLLGQSTLTNQHDGSFVSDITVSGRDLKLRVKPLSQRNPFTLAELYNFSLPETIDL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9707 - -


  Protein sequence: 1132 a.a.    

>T6CP015918 NZ_CP048694:c1650141-1646746 [Vibrio coralliilyticus OCN008] [TssM]
MLKNIIRHPIFISTLLALVLIGGLTAAYFLYPETVSTNILYIGIAAIALVLCAYQLVLFFKKRKQNRDQQLETEEEALSL
VLRPLLVGSKKKPIYLMIGNKAAGKKQFLTSSNAIKSIDTSRTKKNDFFEWYESDEAVYLKPDQRLVFQEVSAADSLLWD
TMVAEVIRSRPRKPFAGCLFFTDLEFLIISEEEQIDYALTALIDRITSICDQTNTALPVYMVVSKLDKLQGFKEYVQLSP
LKHNVEYLSIALKDAKGVLVDYYRDSFNNLVKVIEKNALDSTSQSNDTKEKQAILSFPKQLEICQKEVEYVVQRLSEVNR
GAYFIDLRELFFSSCLQGGRKYNLLAKSCSTYFNLPIIASEHTQLSETPYFTRFLVESQILPEADFAGENKTYLKRILRQ
SRLAMVASVVFLAGGAYLLGTTLDSNLRVIYQLLNIETGDQAADHVRLLDDKLAQAITQIEPVYDAWVEGSEALDEEIVS
LNVSRLEPATKMAYQTLLQSIQKELIPVIEQTYRVQLTKYQDKPIQALPVLKGYLMLKEPSKRDLPFMTTQTQSTLSKVI
GDSEQVASAMKYLNAYFRTDFAPVNINMDIVRATRRNLLAQSNVDLVYAQLINQANSIDLGTLNIERAVGFDFNNVFNDQ
IDQELLDISKVYTATGFSTFYRPRVDLMSKDVITNNWVLGLSKNVTPSKEEHEAFKDQIRKKYTDDYISYWRNALSELKV
KKYNSIGELTNAIDLVSGPSSPLTTVLKQVYSNTHFSPTGEKELLVNKLPTAATEAIDKLADSAEEFVQPDYLLMQRVEQ
AFFQLNRLQINETPNSPTPWDEIVTALSRLRTYMKDIADSPDPQMASLLAARSRMQSSEADPIIRLKQIAQKSPEPVRSW
LLGMVQQSWSVMIAESAKGVQTQWYSEVYTKYRDLALNKYPFDLNAEEEIALEDFELLFSTGGIIDTFVQKNLASFYDTN
LWTPKRVDGETMPLTPELLVQLKNYNVIRDTLINKSTNRVSIPFTTKILDLDSSAIRATVKVADSQMSYYHGPSRIRELE
WPPKSGDFNVSITIQDVTDEGKQYVLSENGQWAIYRLLGQSTLTNQHDGSFVSDITVSGRDLKLRVKPLSQRNPFTLAEL
YNFSLPETIDL*

  Nucleotide sequence: 3396 bp    

>T6CP015918 NZ_CP048694:c1650141-1646746 [Vibrio coralliilyticus OCN008] [TssM]
ATGCTAAAAAATATTATACGTCATCCGATATTCATCTCCACTTTGCTTGCGTTAGTTCTCATTGGTGGTTTAACGGCTGC
ATATTTCTTATACCCAGAAACCGTTTCAACAAACATTTTGTATATTGGAATTGCGGCCATCGCATTAGTGCTTTGTGCTT
ATCAACTAGTTCTTTTCTTTAAGAAGAGAAAACAAAATCGTGACCAGCAGCTAGAAACAGAAGAAGAGGCCTTGAGCTTA
GTTCTTAGACCTCTACTCGTAGGTTCAAAGAAAAAACCAATCTACTTAATGATCGGTAATAAAGCTGCTGGTAAAAAGCA
GTTTCTTACTAGTTCCAATGCTATCAAATCAATTGATACATCACGTACCAAGAAAAATGACTTTTTCGAATGGTATGAGT
CTGACGAAGCGGTTTACCTAAAACCCGATCAAAGATTGGTGTTCCAAGAAGTTTCCGCGGCTGACTCGCTACTGTGGGAT
ACTATGGTCGCGGAAGTGATTCGCAGCCGTCCACGTAAACCATTTGCAGGTTGTTTATTCTTTACTGACTTAGAATTCTT
AATCATCTCTGAAGAAGAGCAGATCGATTATGCTCTGACGGCACTGATTGACAGAATTACGTCCATTTGCGATCAAACAA
ATACCGCACTTCCAGTCTATATGGTCGTCTCTAAGTTAGATAAGCTTCAGGGCTTCAAAGAATATGTCCAATTGAGCCCT
CTTAAGCACAACGTTGAATATTTATCCATCGCACTTAAGGACGCTAAAGGTGTTTTGGTCGACTACTATCGCGATAGTTT
CAACAACTTGGTAAAAGTAATTGAAAAAAATGCGTTGGATTCAACATCACAGTCAAATGACACCAAAGAAAAACAAGCGA
TACTATCTTTCCCGAAACAATTAGAAATTTGTCAGAAGGAAGTTGAGTACGTTGTTCAGCGTCTAAGTGAAGTTAACCGC
GGAGCGTACTTCATTGACCTTCGCGAACTGTTTTTCTCTTCATGTCTTCAGGGTGGCAGAAAGTACAACCTGCTAGCGAA
AAGCTGTAGCACATACTTTAACTTGCCTATTATCGCTTCGGAGCACACTCAGTTATCTGAAACGCCTTATTTCACACGCT
TTCTTGTGGAGTCTCAGATTCTTCCAGAAGCGGATTTTGCAGGTGAAAACAAAACCTACCTGAAGCGAATACTGCGTCAG
AGCCGTTTAGCAATGGTGGCTTCTGTTGTTTTCCTTGCTGGTGGCGCTTATCTACTAGGCACAACACTCGATAGTAACCT
TCGCGTAATCTATCAGCTGTTAAACATTGAAACAGGCGATCAAGCTGCAGACCATGTACGTTTGCTAGATGACAAGTTAG
CACAAGCCATCACCCAGATTGAACCTGTCTACGATGCTTGGGTTGAGGGCAGCGAAGCGCTCGACGAAGAGATCGTCTCT
CTGAATGTGAGCCGCCTTGAGCCTGCGACTAAGATGGCGTACCAAACGCTCCTTCAATCAATTCAGAAAGAACTGATCCC
TGTGATTGAGCAAACTTACCGTGTGCAGCTAACCAAGTATCAGGACAAGCCAATTCAAGCCCTGCCAGTGCTTAAAGGCT
ATTTGATGTTGAAAGAGCCAAGTAAACGCGATCTGCCGTTTATGACAACTCAAACGCAATCGACCCTTTCAAAAGTCATC
GGCGATTCTGAACAGGTAGCCTCTGCAATGAAGTACCTGAATGCTTATTTCAGAACAGACTTCGCACCTGTAAACATCAA
CATGGACATCGTGCGTGCTACACGCAGAAACTTGCTTGCACAATCTAATGTCGACCTTGTTTATGCTCAATTGATCAACC
AAGCGAACTCTATTGACCTTGGTACTTTAAACATTGAACGCGCGGTTGGTTTTGACTTTAACAATGTATTTAACGACCAA
ATTGACCAAGAATTGCTAGATATTAGCAAGGTCTACACAGCCACCGGATTTAGTACCTTCTATCGTCCACGCGTCGATTT
GATGTCAAAAGACGTGATTACAAACAACTGGGTTCTGGGGTTATCTAAGAATGTCACACCTTCAAAAGAAGAGCATGAAG
CGTTCAAAGACCAGATTCGTAAGAAGTATACCGATGACTATATCAGCTACTGGCGTAACGCACTCAGTGAGCTAAAAGTT
AAAAAGTACAATTCAATTGGCGAACTGACTAACGCTATCGACCTTGTTTCTGGCCCTTCTTCACCTCTAACGACGGTACT
TAAGCAGGTGTATTCCAATACGCACTTCTCTCCAACAGGAGAAAAGGAGCTACTAGTGAATAAACTGCCTACAGCAGCAA
CCGAAGCGATAGATAAACTAGCAGATTCAGCAGAGGAATTTGTTCAACCTGATTACTTGCTGATGCAACGCGTCGAACAG
GCTTTCTTCCAGCTTAACCGTCTGCAAATTAACGAGACACCAAACTCCCCTACTCCATGGGATGAGATAGTGACTGCGTT
GAGTCGCCTACGTACCTACATGAAGGACATCGCCGACTCACCAGATCCACAAATGGCCTCGCTACTGGCAGCGCGTTCAC
GCATGCAAAGTTCAGAAGCTGACCCGATTATCCGCCTCAAGCAGATTGCTCAAAAGTCTCCGGAGCCGGTACGCAGTTGG
CTGCTTGGTATGGTTCAGCAAAGTTGGTCTGTCATGATCGCAGAGTCAGCAAAAGGCGTTCAAACTCAATGGTATAGTGA
GGTTTACACTAAGTATCGTGATTTAGCATTAAACAAGTATCCATTTGATTTAAATGCAGAAGAAGAAATTGCACTAGAAG
ACTTCGAGCTTCTTTTCTCTACCGGTGGCATCATCGATACCTTCGTACAAAAGAACTTGGCGTCCTTCTACGACACTAAC
CTTTGGACACCGAAGCGTGTTGATGGCGAGACAATGCCTTTGACGCCTGAGCTTCTGGTCCAATTGAAAAACTACAATGT
GATTCGTGACACGTTGATTAACAAGAGTACCAACCGTGTTTCAATCCCATTTACTACTAAGATTCTCGACCTAGACTCCA
GCGCGATTCGTGCAACAGTCAAAGTTGCAGATTCGCAAATGAGCTACTACCACGGTCCAAGTCGCATTAGGGAACTGGAA
TGGCCACCTAAGAGCGGTGACTTTAACGTGAGTATTACTATCCAAGACGTAACTGATGAGGGCAAACAATACGTACTAAG
CGAAAATGGCCAGTGGGCGATCTACAGGCTGTTGGGCCAATCCACACTAACAAATCAACATGATGGAAGTTTTGTTAGTG
ACATTACCGTTTCAGGTCGTGATCTGAAACTACGAGTGAAGCCACTCTCGCAGAGGAACCCATTTACCCTTGCGGAGCTG
TATAACTTCTCTCTACCCGAAACCATCGACCTATAA