SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015913
Component protein type	TssB
T6SS ID (Type)	T6SS01281 (Type i1)
Strain	Vibrio coralliilyticus OCN008
Replicon	chromosome II
Sequence	Protein sequence (170 a.a.); Nucleotide sequence (510 bp)
Reference	[1] Guillemette R, Ushijima B, Jalan M, et al. Insight into the resilience and susceptibility of marine bacteria to T6SS attack by Vibrio cholerae and Vibrio coralliilyticus. PLoS One. 2020 Jan 28;15(1):e0227864. doi: 10.1371/journal.pone.0227864. eCollection 2020. PMID: 31990915

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	G3U99_RS23845
Coordinate (Strand)	1640957..1641466 (+)
NCBI ID	WP_006961144.1
RefSeq	NZ_CP048694
Uniprot ID	A0A086WMI7_9VIBR, A0A097AXY0_9VIBR, A0A6B8NI90_9VIBR, A0A649YYQ8_9VIBR
KEGG ID	vct:JV59_19710
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	1.9e-58	6..157

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-169	MSRDGSVAPKERINIRYVPATGDAQEDVELPLNMMVVGDFTARADETPIEERTPINIDKDNFNEVLEGYAPNLKVNVENRLTDEEGAQLGVDLTFTNMKDFSPEAIAKSVPELNSLLELREALVALKGPLGNVPAFRKKIAAVLQDEEARKKLLEELSIGENQDAEKAE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9860	-	-

Protein sequence: 170 a.a.

						>T6CP015913 NZ_CP048694:1640957-1641466 [Vibrio coralliilyticus OCN008] [TssB]
MSRDGSVAPKERINIRYVPATGDAQEDVELPLNMMVVGDFTARADETPIEERTPINIDKDNFNEVLEGYAPNLKVNVENR
LTDEEGAQLGVDLTFTNMKDFSPEAIAKSVPELNSLLELREALVALKGPLGNVPAFRKKIAAVLQDEEARKKLLEELSIG
ENQDAEKAE*					

Nucleotide sequence: 510 bp

						>T6CP015913 NZ_CP048694:1640957-1641466 [Vibrio coralliilyticus OCN008] [TssB]
ATGTCACGCGATGGCTCGGTAGCTCCTAAAGAGCGAATTAATATCCGTTACGTTCCAGCGACTGGTGATGCGCAAGAAGA
CGTTGAACTGCCGTTGAATATGATGGTTGTTGGTGACTTTACAGCACGTGCTGATGAGACACCAATCGAAGAACGTACGC
CAATCAATATTGACAAAGACAATTTTAACGAAGTTTTGGAAGGCTATGCTCCAAACCTAAAAGTTAATGTTGAAAACCGT
CTGACTGATGAAGAAGGTGCACAGCTTGGCGTTGATCTAACGTTTACGAACATGAAGGACTTCTCACCTGAGGCGATTGC
TAAAAGTGTTCCGGAACTAAACAGCCTGCTAGAGCTTCGTGAAGCATTGGTTGCGCTAAAAGGTCCACTAGGCAACGTAC
CTGCATTCCGTAAGAAGATCGCTGCCGTTCTTCAGGATGAAGAAGCTCGTAAGAAATTACTAGAAGAACTCAGCATTGGT
GAAAACCAAGATGCTGAAAAGGCTGAATAA					