SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015911
Component protein type	TssI
T6SS ID (Type)	T6SS01281 (Type i1)
Strain	Vibrio coralliilyticus OCN008
Replicon	chromosome II
Sequence	Protein sequence (693 a.a.); Nucleotide sequence (2079 bp)
Reference	[1] Guillemette R, Ushijima B, Jalan M, et al. Insight into the resilience and susceptibility of marine bacteria to T6SS attack by Vibrio cholerae and Vibrio coralliilyticus. PLoS One. 2020 Jan 28;15(1):e0227864. doi: 10.1371/journal.pone.0227864. eCollection 2020. PMID: 31990915

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	G3U99_RS23810
Coordinate (Strand)	1631338..1633416 (+)
NCBI ID	WP_040121702.1
RefSeq	NZ_CP048694
Uniprot ID	A0A2A2MZG2_9VIBR
KEGG ID	vct:JV59_19745
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	5.9e-78	26..331
Phage_base_V	PF04717.14	8.8e-15	387..455

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-692	MTIDVEFKFEVQGSGHEFRVESFQVTEELSKPFQVSLSLLSLDADITFDELIRKPALLTLFGQGVGSARLFHGVVNEVRFLGSGRRFSRYQIILVPQMWFMTQRQDCRIFQNLAAPDIISQVLDDASVTDYRLDLSGVYPPKEYVLQYRESDSNFVQRMLAEHGMWYYFEHTEGNHTMVIVDSNDAIPELLSTPLNASYLGPVVYHSDDGGVADREHIFDLAAINRVRTGSTTYTDYNYEQPKIPQEMSSDGPLDQDLKQFDYPGRYVDPAMGQVRASEWIADHQVENQQIEAASNIMRMVPGYSFNIGEHPRSAINRDYTMLSVIHSGHDPQVHEDENSGLPTTYQNQFICIPRDVIFKAPKLPAPMVDGPQTAVVVGPAGEEIYTDKLGRIKVQFHWDRYGNNDEHASCWIRVSQSMAAPTWGAVYLPRIGHEVVVTFLEGDPDRPLVTGAVYNGLHYPPYSLPENKTRTTFRTQTHKGTGYNEMSFEDEANQEEIYFHAQKDMSTKVLNNRYRDIGQDEFLKVGRHQTNEVHGDHKETIDGHKTTQVNSTFTETVEQDVTVTYNANETQSVKNNSTLEIGDNRSTKIGKNDDLDVGENSNLTVGASRSADIGADDNLTVGGNLTVSVKGNTSYKADAATQVISGDKIVLKTGGSSLVMNSDGSIKLSGSSITVEGSDKVVIKGGNVAIN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9937	-	-

Protein sequence: 693 a.a.

						>T6CP015911 NZ_CP048694:1631338-1633416 [Vibrio coralliilyticus OCN008] [TssI]
MTIDVEFKFEVQGSGHEFRVESFQVTEELSKPFQVSLSLLSLDADITFDELIRKPALLTLFGQGVGSARLFHGVVNEVRF
LGSGRRFSRYQIILVPQMWFMTQRQDCRIFQNLAAPDIISQVLDDASVTDYRLDLSGVYPPKEYVLQYRESDSNFVQRML
AEHGMWYYFEHTEGNHTMVIVDSNDAIPELLSTPLNASYLGPVVYHSDDGGVADREHIFDLAAINRVRTGSTTYTDYNYE
QPKIPQEMSSDGPLDQDLKQFDYPGRYVDPAMGQVRASEWIADHQVENQQIEAASNIMRMVPGYSFNIGEHPRSAINRDY
TMLSVIHSGHDPQVHEDENSGLPTTYQNQFICIPRDVIFKAPKLPAPMVDGPQTAVVVGPAGEEIYTDKLGRIKVQFHWD
RYGNNDEHASCWIRVSQSMAAPTWGAVYLPRIGHEVVVTFLEGDPDRPLVTGAVYNGLHYPPYSLPENKTRTTFRTQTHK
GTGYNEMSFEDEANQEEIYFHAQKDMSTKVLNNRYRDIGQDEFLKVGRHQTNEVHGDHKETIDGHKTTQVNSTFTETVEQ
DVTVTYNANETQSVKNNSTLEIGDNRSTKIGKNDDLDVGENSNLTVGASRSADIGADDNLTVGGNLTVSVKGNTSYKADA
ATQVISGDKIVLKTGGSSLVMNSDGSIKLSGSSITVEGSDKVVIKGGNVAIN*					

Nucleotide sequence: 2079 bp

						>T6CP015911 NZ_CP048694:1631338-1633416 [Vibrio coralliilyticus OCN008] [TssI]
ATGACGATTGACGTAGAATTTAAATTTGAAGTTCAGGGTAGTGGCCATGAATTTCGTGTTGAAAGTTTTCAAGTGACTGA
AGAACTGTCGAAACCATTTCAAGTCAGCCTCTCATTACTTTCACTTGATGCTGATATTACGTTCGACGAACTTATTCGCA
AACCAGCATTACTGACATTGTTTGGTCAGGGTGTTGGTTCTGCTCGTTTGTTCCATGGCGTAGTAAATGAGGTACGATTC
CTTGGCTCAGGCCGCAGGTTCTCGCGCTACCAGATTATTCTTGTTCCACAGATGTGGTTCATGACCCAACGCCAAGATTG
TCGAATTTTCCAGAATTTAGCCGCACCAGACATCATTTCTCAGGTGCTAGATGACGCCTCTGTCACCGATTACCGTCTTG
ACCTTTCTGGTGTTTATCCTCCTAAGGAATATGTGCTTCAGTACCGTGAAAGCGACAGTAACTTTGTTCAACGTATGCTT
GCTGAGCATGGTATGTGGTACTACTTCGAGCATACAGAAGGCAACCACACAATGGTCATTGTTGATAGCAATGACGCGAT
TCCAGAGCTGTTAAGTACACCGCTGAACGCATCCTACCTAGGTCCGGTGGTGTATCACTCGGATGACGGCGGCGTGGCTG
ACCGTGAACATATCTTTGACCTTGCAGCGATCAACCGAGTTAGAACCGGTAGTACAACATATACCGACTACAACTATGAA
CAACCTAAGATCCCTCAGGAAATGTCTAGTGATGGTCCTTTAGATCAGGATCTTAAGCAGTTTGACTACCCTGGTCGTTA
TGTTGACCCTGCAATGGGACAAGTCCGTGCGTCAGAGTGGATTGCTGATCATCAGGTAGAAAATCAGCAGATTGAAGCGG
CGTCTAACATCATGAGAATGGTGCCGGGTTACAGCTTTAATATTGGTGAGCACCCACGTAGCGCGATTAACCGTGATTAC
ACTATGTTGTCAGTTATTCATAGTGGTCATGATCCTCAGGTGCATGAGGACGAAAACAGCGGCTTGCCAACTACTTATCA
GAACCAGTTTATCTGTATCCCACGTGATGTGATTTTCAAAGCACCTAAACTGCCAGCACCTATGGTTGATGGTCCTCAGA
CTGCGGTTGTTGTTGGCCCTGCGGGCGAAGAAATCTACACGGATAAACTTGGCCGAATCAAGGTTCAATTTCATTGGGAC
AGATACGGTAACAATGATGAGCATGCTAGCTGCTGGATTCGTGTCAGCCAATCAATGGCAGCCCCAACTTGGGGTGCCGT
TTATCTGCCGCGTATTGGGCACGAAGTTGTGGTTACCTTCCTCGAGGGAGACCCAGATCGTCCACTAGTAACAGGCGCGG
TATACAACGGTCTACATTACCCTCCTTACTCATTGCCTGAGAATAAAACGCGTACGACATTCCGAACTCAGACCCATAAG
GGAACGGGTTACAACGAGATGAGCTTCGAAGACGAAGCAAATCAGGAAGAAATCTACTTCCATGCTCAGAAAGATATGTC
GACCAAGGTGCTGAATAACCGTTACCGTGATATTGGCCAGGATGAGTTTCTTAAAGTAGGGCGCCATCAGACAAACGAAG
TGCATGGTGATCACAAGGAAACCATCGACGGACATAAAACGACTCAGGTGAACAGTACGTTTACTGAGACCGTTGAACAA
GATGTCACGGTAACTTACAACGCAAACGAAACTCAATCGGTTAAGAATAACTCGACACTTGAAATAGGTGACAACCGCTC
AACTAAGATAGGCAAAAACGACGATCTAGATGTTGGTGAAAACAGCAACCTGACTGTCGGTGCTTCTCGCTCCGCTGATA
TTGGTGCTGATGACAATCTAACAGTCGGTGGCAACCTTACAGTCTCTGTGAAAGGAAACACGTCATACAAAGCGGACGCT
GCAACACAGGTGATCAGTGGTGACAAAATCGTGCTGAAAACAGGTGGCTCTTCTTTGGTTATGAACAGTGATGGAAGTAT
CAAGCTTTCCGGTTCTTCTATTACAGTTGAAGGCAGCGACAAAGTTGTGATTAAAGGTGGCAACGTAGCGATTAACTAA					