SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015905
Component protein type	TssJ
T6SS ID (Type)	T6SS01280 (Type i5)
Strain	Vibrio coralliilyticus OCN008
Replicon	chromosome I
Sequence	Protein sequence (161 a.a.); Nucleotide sequence (483 bp)
Reference	[1] Guillemette R, Ushijima B, Jalan M, et al. Insight into the resilience and susceptibility of marine bacteria to T6SS attack by Vibrio cholerae and Vibrio coralliilyticus. PLoS One. 2020 Jan 28;15(1):e0227864. doi: 10.1371/journal.pone.0227864. eCollection 2020. PMID: 31990915

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	G3U99_RS13195
Coordinate (Strand)	2781080..2781562 (+)
NCBI ID	WP_006962078.1
RefSeq	NZ_CP048693
Uniprot ID	A0A086WRM7_9VIBR, A0A0A0SNZ5_9VIBR, A0A6B8N7L9_9VIBR, A0A649YPC2_9VIBR
KEGG ID	vcy:IX92_06565
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	3.5e-26	24..141

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-160	MAKWCFVLALFLVGCSSDPAPVVTQYSLAIKSDKSANPSESNDSNPVVVRLYQLTDAQMFKQQPFIDLYSNDVQLLSANLISKQILPVVIPGSEEKITLDINNQTQYVGVLVEFIDYQQSEPKSVSMLPKNSDEYLQLSISGDKAKLEVITPDSPWWKLF

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9988	pos: 14-15. LVG-CS.	MAKWCFVLALFLVG

Protein sequence: 161 a.a.

						>T6CP015905 NZ_CP048693:2781080-2781562 [Vibrio coralliilyticus OCN008] [TssJ]
MAKWCFVLALFLVGCSSDPAPVVTQYSLAIKSDKSANPSESNDSNPVVVRLYQLTDAQMFKQQPFIDLYSNDVQLLSANL
ISKQILPVVIPGSEEKITLDINNQTQYVGVLVEFIDYQQSEPKSVSMLPKNSDEYLQLSISGDKAKLEVITPDSPWWKLF
*					

Nucleotide sequence: 483 bp

						>T6CP015905 NZ_CP048693:2781080-2781562 [Vibrio coralliilyticus OCN008] [TssJ]
ATGGCTAAGTGGTGTTTTGTTTTGGCTCTGTTTCTGGTGGGTTGTTCATCCGATCCAGCGCCTGTTGTTACACAATACTC
ATTAGCAATTAAGTCAGACAAATCGGCTAACCCTTCGGAGTCTAACGATTCCAACCCTGTGGTTGTACGCCTTTACCAGC
TGACTGATGCACAAATGTTTAAACAGCAGCCTTTTATTGACCTGTACAGCAATGACGTACAGTTACTTAGCGCTAACTTG
ATTTCCAAGCAAATTTTGCCAGTGGTCATTCCTGGGAGCGAGGAGAAAATAACTTTAGATATCAATAACCAAACACAATA
CGTCGGTGTTTTGGTGGAGTTTATTGACTATCAACAAAGTGAGCCAAAGTCGGTGTCAATGTTACCTAAAAATTCAGACG
AGTATTTGCAACTTAGCATTTCAGGTGACAAAGCCAAGCTCGAAGTTATTACGCCAGATTCTCCCTGGTGGAAATTATTT
TAA					