SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015902
Component protein type	TssF
T6SS ID (Type)	T6SS01280 (Type i5)
Strain	Vibrio coralliilyticus OCN008
Replicon	chromosome I
Sequence	Protein sequence (584 a.a.); Nucleotide sequence (1752 bp)
Reference	[1] Guillemette R, Ushijima B, Jalan M, et al. Insight into the resilience and susceptibility of marine bacteria to T6SS attack by Vibrio cholerae and Vibrio coralliilyticus. PLoS One. 2020 Jan 28;15(1):e0227864. doi: 10.1371/journal.pone.0227864. eCollection 2020. PMID: 31990915

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	G3U99_RS13160
Coordinate (Strand)	2770467..2772218 (+)
NCBI ID	WP_021457560.1
RefSeq	NZ_CP048693
Uniprot ID	UPI0003917754
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	4.7e-127	1..580

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-583	MTDPLMRYFEQELTFVRRALGQFGQQHSEHAERLNIHQGQIEDPSLARLLDGVALLNATVEKQLSEQLPEVVEGLLGVLYPSYIQTIPSVAYLQLAQEEDVPESVLIPKGSHFTSEAKGRLCQFTTVDDLKTAPFTLTDTTASSAPFKFNRPAGTEQSTAVIQLSFSTGDPDTHFSQLGHQDLDLFVQGFENNADSLVELLLGQTKAISISDASCEKHVVLLAEKLKSRISDLNFLFLPQKGNQFSGFQLISEFFFFKEKRQFFRLKDFGQAIKQFDCSEIKLNLFMNSIPTEFMRLFDNQVFKLDIIPAVNLFEQTGEPTNYDQRSLTIPVNADAHSESDIEVVEVKEVFEITPQGEKPLVPLFRDSYKSNPNADYWQSSRDINGQFQLAISLKPNHDIEFNKLYGTHLLCTNGKQACGINGEMECLESIDLPGVFRAIYPPTAPIEREQNLNLHWQFVGLLNGNFSSLLHADNPTEKLKQMLQLCSREQVSAEEIQTIHNVSIKSQVSAIRIMGKNVFSPGTEIELTLDTQGGFLVFSDVLNRFFQQFCSFDRYIQLSIKIYGRDGIAKRYPKIHGSQLAM

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9919	-	-

Protein sequence: 584 a.a.

						>T6CP015902 NZ_CP048693:2770467-2772218 [Vibrio coralliilyticus OCN008] [TssF]
MTDPLMRYFEQELTFVRRALGQFGQQHSEHAERLNIHQGQIEDPSLARLLDGVALLNATVEKQLSEQLPEVVEGLLGVLY
PSYIQTIPSVAYLQLAQEEDVPESVLIPKGSHFTSEAKGRLCQFTTVDDLKTAPFTLTDTTASSAPFKFNRPAGTEQSTA
VIQLSFSTGDPDTHFSQLGHQDLDLFVQGFENNADSLVELLLGQTKAISISDASCEKHVVLLAEKLKSRISDLNFLFLPQ
KGNQFSGFQLISEFFFFKEKRQFFRLKDFGQAIKQFDCSEIKLNLFMNSIPTEFMRLFDNQVFKLDIIPAVNLFEQTGEP
TNYDQRSLTIPVNADAHSESDIEVVEVKEVFEITPQGEKPLVPLFRDSYKSNPNADYWQSSRDINGQFQLAISLKPNHDI
EFNKLYGTHLLCTNGKQACGINGEMECLESIDLPGVFRAIYPPTAPIEREQNLNLHWQFVGLLNGNFSSLLHADNPTEKL
KQMLQLCSREQVSAEEIQTIHNVSIKSQVSAIRIMGKNVFSPGTEIELTLDTQGGFLVFSDVLNRFFQQFCSFDRYIQLS
IKIYGRDGIAKRYPKIHGSQLAM*					

Nucleotide sequence: 1752 bp

						>T6CP015902 NZ_CP048693:2770467-2772218 [Vibrio coralliilyticus OCN008] [TssF]
ATGACTGATCCTTTAATGCGTTACTTCGAGCAAGAGCTTACTTTCGTAAGACGTGCACTAGGTCAATTTGGCCAACAACA
CTCAGAACATGCAGAGCGATTAAATATTCATCAGGGTCAGATTGAAGACCCAAGCCTCGCTCGATTACTGGATGGCGTTG
CCCTCCTCAATGCGACGGTAGAAAAACAGTTATCGGAACAATTACCAGAAGTAGTCGAAGGCCTATTAGGTGTGCTCTAC
CCGAGCTATATTCAAACTATTCCGAGTGTCGCATACCTACAGTTGGCGCAAGAAGAAGATGTGCCGGAGTCAGTTTTAAT
ACCTAAAGGCTCACACTTTACTTCAGAAGCTAAGGGCCGATTGTGTCAGTTTACGACTGTAGACGATCTCAAAACTGCGC
CTTTTACATTGACCGATACAACAGCCTCCAGCGCACCGTTTAAATTCAATCGTCCCGCTGGTACCGAGCAGTCTACCGCA
GTGATACAGCTGTCGTTTTCCACTGGGGATCCAGATACTCATTTTTCTCAGCTCGGTCACCAAGACCTAGATCTATTTGT
TCAGGGTTTCGAGAATAATGCCGACTCTCTGGTGGAGCTCTTGTTGGGGCAAACCAAAGCGATTTCGATATCGGATGCCA
GTTGCGAGAAGCACGTTGTCTTATTAGCAGAAAAACTTAAAAGTCGGATAAGTGACCTGAATTTCCTGTTCCTTCCTCAA
AAAGGAAATCAATTTTCAGGCTTTCAACTTATTAGCGAATTTTTCTTCTTCAAAGAAAAGCGCCAGTTTTTCCGTCTGAA
AGATTTCGGACAAGCTATAAAGCAGTTTGATTGCTCCGAAATCAAACTGAACCTGTTTATGAATTCAATTCCTACAGAAT
TCATGCGCTTATTCGATAATCAAGTGTTTAAGCTGGATATAATCCCAGCGGTTAACTTGTTTGAACAAACAGGTGAACCA
ACAAATTATGACCAACGCTCTCTGACTATTCCTGTCAATGCAGATGCTCACAGTGAAAGTGACATTGAAGTTGTCGAAGT
AAAAGAAGTCTTTGAGATCACCCCTCAAGGTGAAAAGCCTCTGGTTCCACTATTCAGGGATAGCTATAAATCCAACCCAA
ATGCCGATTATTGGCAGAGTTCAAGGGATATTAATGGTCAATTCCAACTTGCCATCAGCCTAAAGCCAAACCATGATATA
GAGTTTAACAAGCTCTATGGGACACATTTGCTTTGTACCAATGGAAAACAAGCATGTGGTATTAATGGAGAGATGGAGTG
TCTTGAAAGCATTGACCTTCCTGGCGTATTTCGTGCTATTTACCCACCTACGGCTCCGATTGAACGAGAACAGAACCTCA
ATCTTCACTGGCAGTTTGTTGGCTTATTGAATGGTAATTTTTCTTCACTGTTACATGCGGATAACCCGACAGAGAAGCTA
AAGCAAATGCTGCAGCTATGTAGCCGAGAGCAAGTATCCGCGGAAGAAATACAAACGATTCACAATGTATCCATCAAATC
TCAGGTGTCTGCCATTCGAATTATGGGTAAAAACGTCTTTTCTCCAGGTACAGAGATTGAACTCACACTCGATACTCAAG
GGGGCTTTCTGGTCTTTAGCGATGTTCTTAATCGTTTCTTCCAACAGTTTTGCAGTTTTGATCGCTATATCCAGCTGTCT
ATTAAGATATACGGGCGAGATGGCATCGCAAAACGCTACCCTAAAATCCATGGGAGCCAGCTAGCAATGTAG					