SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015899
Component protein type	TssB
T6SS ID (Type)	T6SS01280 (Type i5)
Strain	Vibrio coralliilyticus OCN008
Replicon	chromosome I
Sequence	Protein sequence (169 a.a.); Nucleotide sequence (507 bp)
Reference	[1] Guillemette R, Ushijima B, Jalan M, et al. Insight into the resilience and susceptibility of marine bacteria to T6SS attack by Vibrio cholerae and Vibrio coralliilyticus. PLoS One. 2020 Jan 28;15(1):e0227864. doi: 10.1371/journal.pone.0227864. eCollection 2020. PMID: 31990915

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	G3U99_RS13140
Coordinate (Strand)	2766615..2767121 (+)
NCBI ID	WP_006962089.1
RefSeq	NZ_CP048693
Uniprot ID	A0A086WRN8_9VIBR, A0A097QKF1_9VIBR, A0A7Y3ZP66_9VIBR, A0A6B8MWN1_9VIBR, A0A649YPE6_9VIBR
KEGG ID	vct:JV59_31905, vcy:IX92_06620
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	5.5e-57	8..159

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-168	VALNSQHKRVSKNRVSITYDVETNGAVETKELPFVVGVIGDYSGHKQDKEDVEDRTFYNVDKDNFDTVMKRVGPELSLKVDNVLADDDSQFEAALKFNSMKDFEPEAIVEQVEPLKKLVETRNQLKVLLSKADRSRDLEKLLKEVLQSADTINALSEELGVNKEGGEE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9916	-	-

Protein sequence: 169 a.a.

						>T6CP015899 NZ_CP048693:2766615-2767121 [Vibrio coralliilyticus OCN008] [TssB]
VALNSQHKRVSKNRVSITYDVETNGAVETKELPFVVGVIGDYSGHKQDKEDVEDRTFYNVDKDNFDTVMKRVGPELSLKV
DNVLADDDSQFEAALKFNSMKDFEPEAIVEQVEPLKKLVETRNQLKVLLSKADRSRDLEKLLKEVLQSADTINALSEELG
VNKEGGEE*					

Nucleotide sequence: 507 bp

						>T6CP015899 NZ_CP048693:2766615-2767121 [Vibrio coralliilyticus OCN008] [TssB]
GTGGCACTTAATTCCCAACACAAACGTGTAAGTAAAAACCGCGTAAGTATTACTTACGATGTGGAAACTAATGGCGCTGT
AGAAACCAAAGAACTCCCATTTGTGGTTGGGGTAATTGGTGACTACTCTGGTCATAAACAAGACAAGGAAGACGTTGAAG
ATCGTACGTTCTACAACGTTGACAAAGACAACTTTGACACCGTGATGAAACGCGTTGGCCCAGAGTTGTCTTTGAAAGTC
GACAATGTACTTGCAGACGATGATAGCCAGTTTGAAGCTGCTTTGAAGTTCAACTCAATGAAAGACTTTGAACCTGAAGC
TATCGTCGAGCAAGTAGAACCTCTTAAAAAGCTTGTTGAAACCCGTAACCAGCTAAAAGTACTACTTTCAAAAGCAGATC
GCTCTCGCGATCTCGAGAAGCTGCTGAAAGAAGTGCTCCAAAGTGCAGATACTATCAATGCATTATCGGAAGAGCTTGGT
GTCAATAAGGAAGGAGGCGAAGAATGA					