SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015898
Component protein type	TssD
T6SS ID (Type)	T6SS01280 (Type i5)
Strain	Vibrio coralliilyticus OCN008
Replicon	chromosome I
Sequence	Protein sequence (174 a.a.); Nucleotide sequence (522 bp)
Reference	[1] Guillemette R, Ushijima B, Jalan M, et al. Insight into the resilience and susceptibility of marine bacteria to T6SS attack by Vibrio cholerae and Vibrio coralliilyticus. PLoS One. 2020 Jan 28;15(1):e0227864. doi: 10.1371/journal.pone.0227864. eCollection 2020. PMID: 31990915

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	G3U99_RS13135
Coordinate (Strand)	2766074..2766595 (+)
NCBI ID	WP_006962090.1
RefSeq	NZ_CP048693
Uniprot ID	A0A086WRN9_9VIBR, A0A097QKF4_9VIBR, A0A7Y3ZND5_9VIBR, A0A6B8N5G7_9VIBR, A0A649YPK1_9VIBR
KEGG ID	vcy:IX92_06625, vct:JV59_31900
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_HCP	PF05638.14	3.5e-18	5..144

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-173	MASIFMRIDGTTPKGAATVEKIGGKDGFFAIDTVTWNAVRGVGIDVGNANNADQGMVALGEINVTRGCDGATPHLTTFLYAPGGEGKTVEIVMTKPNREGSGADPYLIVTLKAARMSSYNMAGSDGTLPNESFSLTYTEISKAYYIEGEGGKIEKGPEVGFDATTAKVTSTAA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9786	-	-

Protein sequence: 174 a.a.

						>T6CP015898 NZ_CP048693:2766074-2766595 [Vibrio coralliilyticus OCN008] [TssD]
MASIFMRIDGTTPKGAATVEKIGGKDGFFAIDTVTWNAVRGVGIDVGNANNADQGMVALGEINVTRGCDGATPHLTTFLY
APGGEGKTVEIVMTKPNREGSGADPYLIVTLKAARMSSYNMAGSDGTLPNESFSLTYTEISKAYYIEGEGGKIEKGPEVG
FDATTAKVTSTAA*					

Nucleotide sequence: 522 bp

						>T6CP015898 NZ_CP048693:2766074-2766595 [Vibrio coralliilyticus OCN008] [TssD]
ATGGCTTCTATTTTCATGAGAATTGATGGCACTACACCTAAAGGTGCAGCAACTGTAGAAAAGATCGGTGGTAAAGATGG
TTTCTTTGCTATTGACACCGTGACTTGGAATGCTGTTCGTGGCGTGGGTATTGATGTGGGTAACGCAAACAACGCTGACC
AAGGTATGGTTGCGCTTGGCGAAATCAACGTAACTCGTGGTTGTGACGGTGCAACTCCGCATCTAACAACTTTCCTTTAC
GCACCAGGCGGTGAAGGTAAAACAGTTGAAATCGTAATGACTAAGCCAAACCGTGAAGGTTCTGGTGCAGATCCATACCT
AATCGTAACTCTAAAAGCAGCTCGTATGTCTAGCTACAACATGGCTGGTTCTGACGGTACTCTGCCAAACGAATCTTTCT
CTCTAACCTACACTGAAATTTCGAAAGCTTACTACATCGAAGGTGAAGGCGGTAAGATCGAGAAAGGTCCTGAAGTTGGC
TTCGATGCAACAACTGCGAAAGTTACTTCGACTGCTGCTTAA					