SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015874
Component protein type	TssJ
T6SS ID (Type)	T6SS01277 (Type i1)
Strain	Aliivibrio fischeri strain FQ-A001
Replicon	chromosome
Sequence	Protein sequence (164 a.a.); Nucleotide sequence (492 bp)
Reference	[1] Guckes KR, Cecere AG, Wasilko NP, et al. Incompatibility of Vibrio fischeri Strains during Symbiosis Establishment Depends on Two Functionally Redundant hcp Genes. J Bacteriol. 2019 Sep 6;201(19). pii: e00221-19. doi: 10.1128/JB.00221-19. Print 2019 Oct 1. PMID: 31331977 [2] Speare L, Cecere AG, Guckes KR, et al. Bacterial symbionts use a type VI secretion system to eliminate competitors in their natural host. Proc Natl Acad Sci U S A. 2018 Sep 4;115(36):E8528-E8537. doi: 10.1073/pnas.1808302115. Epub 2018 Aug 20. PMID: 30127013

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VFFQA001_RS05450
Coordinate (Strand)	753171..753662 (-)
NCBI ID	499580846
RefSeq	NZ_SJSX01000002
Uniprot ID	Q5E655_ALIF1
KEGG ID	vfi:VF_0996
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	1.2e-35	24..139

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-163	MKKLFTFIALLLLIGCSSTPDPTYVALTLNASANVNPDHADRPSPLVVRVLELSSITGFENSEFFDLYNNAKSTLGADFIAEEEIILRPGDKKIIKLKLSEKGQYLAVIGAYQDIDNAKWHYIYTVEPEEAQKVELIATDSSIVKLKRVKKKKNAPQSSNARI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9990	pos: 15-16. LIG-CS.	MKKLFTFIALLLLIG

Protein sequence: 164 a.a.

						>T6CP015874 NZ_SJSX01000002:c753662-753171 [Aliivibrio fischeri] [TssJ]
MKKLFTFIALLLLIGCSSTPDPTYVALTLNASANVNPDHADRPSPLVVRVLELSSITGFENSEFFDLYNNAKSTLGADFI
AEEEIILRPGDKKIIKLKLSEKGQYLAVIGAYQDIDNAKWHYIYTVEPEEAQKVELIATDSSIVKLKRVKKKKNAPQSSN
ARI*					

Nucleotide sequence: 492 bp

						>T6CP015874 NZ_SJSX01000002:c753662-753171 [Aliivibrio fischeri] [TssJ]
ATGAAAAAATTATTTACCTTCATAGCTCTATTGTTACTCATTGGATGCAGCAGCACACCAGATCCAACTTACGTAGCGCT
TACTCTCAATGCCTCTGCAAATGTTAACCCTGATCATGCGGATCGCCCCTCGCCTCTAGTGGTAAGGGTATTAGAGTTAT
CTTCAATCACTGGCTTTGAAAACTCAGAATTTTTTGATTTATACAATAATGCAAAATCAACCTTAGGAGCGGATTTTATC
GCAGAAGAGGAAATCATCTTACGACCGGGCGATAAGAAAATCATCAAATTAAAGCTCAGTGAAAAAGGACAATATTTAGC
CGTTATCGGCGCTTATCAAGATATAGATAATGCAAAATGGCATTACATATACACAGTTGAGCCCGAAGAGGCACAAAAAG
TGGAATTGATCGCTACCGACTCTTCTATCGTCAAATTAAAACGCGTGAAAAAGAAAAAGAACGCACCACAAAGCAGTAAT
GCGCGAATTTAG					