SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015871
Component protein type	TssM
T6SS ID (Type)	T6SS01277 (Type i1)
Strain	Aliivibrio fischeri strain FQ-A001
Replicon	chromosome
Sequence	Protein sequence (1174 a.a.); Nucleotide sequence (3522 bp)
Reference	[1] Guckes KR, Cecere AG, Wasilko NP, et al. Incompatibility of Vibrio fischeri Strains during Symbiosis Establishment Depends on Two Functionally Redundant hcp Genes. J Bacteriol. 2019 Sep 6;201(19). pii: e00221-19. doi: 10.1128/JB.00221-19. Print 2019 Oct 1. PMID: 31331977 [2] Speare L, Cecere AG, Guckes KR, et al. Bacterial symbionts use a type VI secretion system to eliminate competitors in their natural host. Proc Natl Acad Sci U S A. 2018 Sep 4;115(36):E8528-E8537. doi: 10.1073/pnas.1808302115. Epub 2018 Aug 20. PMID: 30127013

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VFFQA001_RS05435
Coordinate (Strand)	747470..750991 (-)
NCBI ID	1597592722
RefSeq	NZ_SJSX01000002
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related_N	PF14331.8	9.8e-74	203..452
IcmF-related	PF06761.14	3.5e-67	502..795
IcmF_C	PF06744.14	3.9e-19	1046..1150

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-15	MKSFFNSLLNTLKKT
Transmembrane helix	16-38	WCWSLLLTLFIALLLCLFGKHIA
Outside	39-52	IANTPLIETTTGKT
Transmembrane helix	53-72	LTVIICLLLWGAFNLALWGL
Inside	73-446	AKREAQKEENKEAYEAAQSEMAFIREHSTILKTKLEAAIATIKRAGLYGKLNNNVKYQLPWYMVIGPQNSGKTTLLECSGLDFPLNQTDGHYTRDIQNSQHCEWYFANHAVLLDAAGRFFDQHENELSKPIWGNFLKALRNKRRRRPLNGVILTIDITTLQSPDESAIEVQARYVRERLQELRHDLSSDMPIYFLLTKMDKVEGFEPFFSSLSREEMDQVFGVTFNEGEGQQADKIKHEFEKLILRIDSQVMSRLHDERDVMKRAELLQFPRQLSFLAERLALFSELAFTKTRYHQASHLRGLYLTSVPEPTNTQTESTTSGIGQNLGISSRVLPTYKHQRGFFIRKLLEEVIFPNSELATLDEKYEKQVKLKN
Transmembrane helix	447-469	MAVYAASFAIVIGFGSLWATSFL
Outside	470-1173	TNHHKQQELLSLSSDYNQQIVHLSPLAPSIELLPTLNTLLAATQVYTKEGETLTDEFAGLQQGDKLRKAAEAAYHRSLLSLLLPKVTSELEYQVANNQDNREFLTPSLRAYLMLNLHEHLDKAYLEQWMGLHWSYLYSGSATEQKELQNHFSNLLAIEFDPVPLNNNLIAKSRKFLREANTSELVYQQLKQDAKEMDLRDIKISDHLGPNQNLFNHTNTIIPGLYTQKGYKAVFLSKGLDQVKQLIEENWVIGLSSDLSANEIRGLYADVEDLYFHDYIKYWKEAVDQLQIKPSKNMDEAILQISNITGSSQPILKLLNLVKENTTFVDKAQVASKTAGQVKKLPVNTKLKKVASLGLHSVEERSQSARKAVSNQFEPLNLLLTDKDQATIPLEDALVVINELSGRLSMVKFSPNPDSSAFKIARDRMQGIPNELNDIRVMAETLPQPLQQWWKQISNNAWGIILNHSRKHVQMVYHDKVVTHYDVALSGRYPFRNSSHDVNIADFDEFFQNGGILDNFFDAYLSSFVYKQKGKFKQKSLHGRSLGLSDSFLDQYNYGLTIQRMFYGSKGKEANVSFRIAPFELDASALQSTFYYGKNKFAYRHGPIQKKQFTWPITNQRQYVSFVLQDLSGSKVVNQQNSGPWAFFRVLDKFRVNKYRGQDVIKVDLENKGMKAKYLIYSDRTPNPFNRKILTNFYLPKRING

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9750	-	-

Protein sequence: 1174 a.a.

						>T6CP015871 NZ_SJSX01000002:c750991-747470 [Aliivibrio fischeri] [TssM]
MKSFFNSLLNTLKKTWCWSLLLTLFIALLLCLFGKHIAIANTPLIETTTGKTLTVIICLLLWGAFNLALWGLAKREAQKE
ENKEAYEAAQSEMAFIREHSTILKTKLEAAIATIKRAGLYGKLNNNVKYQLPWYMVIGPQNSGKTTLLECSGLDFPLNQT
DGHYTRDIQNSQHCEWYFANHAVLLDAAGRFFDQHENELSKPIWGNFLKALRNKRRRRPLNGVILTIDITTLQSPDESAI
EVQARYVRERLQELRHDLSSDMPIYFLLTKMDKVEGFEPFFSSLSREEMDQVFGVTFNEGEGQQADKIKHEFEKLILRID
SQVMSRLHDERDVMKRAELLQFPRQLSFLAERLALFSELAFTKTRYHQASHLRGLYLTSVPEPTNTQTESTTSGIGQNLG
ISSRVLPTYKHQRGFFIRKLLEEVIFPNSELATLDEKYEKQVKLKNMAVYAASFAIVIGFGSLWATSFLTNHHKQQELLS
LSSDYNQQIVHLSPLAPSIELLPTLNTLLAATQVYTKEGETLTDEFAGLQQGDKLRKAAEAAYHRSLLSLLLPKVTSELE
YQVANNQDNREFLTPSLRAYLMLNLHEHLDKAYLEQWMGLHWSYLYSGSATEQKELQNHFSNLLAIEFDPVPLNNNLIAK
SRKFLREANTSELVYQQLKQDAKEMDLRDIKISDHLGPNQNLFNHTNTIIPGLYTQKGYKAVFLSKGLDQVKQLIEENWV
IGLSSDLSANEIRGLYADVEDLYFHDYIKYWKEAVDQLQIKPSKNMDEAILQISNITGSSQPILKLLNLVKENTTFVDKA
QVASKTAGQVKKLPVNTKLKKVASLGLHSVEERSQSARKAVSNQFEPLNLLLTDKDQATIPLEDALVVINELSGRLSMVK
FSPNPDSSAFKIARDRMQGIPNELNDIRVMAETLPQPLQQWWKQISNNAWGIILNHSRKHVQMVYHDKVVTHYDVALSGR
YPFRNSSHDVNIADFDEFFQNGGILDNFFDAYLSSFVYKQKGKFKQKSLHGRSLGLSDSFLDQYNYGLTIQRMFYGSKGK
EANVSFRIAPFELDASALQSTFYYGKNKFAYRHGPIQKKQFTWPITNQRQYVSFVLQDLSGSKVVNQQNSGPWAFFRVLD
KFRVNKYRGQDVIKVDLENKGMKAKYLIYSDRTPNPFNRKILTNFYLPKRING*					

Nucleotide sequence: 3522 bp

						>T6CP015871 NZ_SJSX01000002:c750991-747470 [Aliivibrio fischeri] [TssM]
ATGAAATCATTTTTTAATTCATTGCTTAACACATTAAAAAAGACATGGTGTTGGAGCTTATTATTAACACTATTTATTGC
CTTATTATTGTGTCTATTTGGTAAGCATATTGCTATAGCAAATACCCCATTAATTGAAACGACTACAGGAAAAACATTAA
CGGTTATCATTTGCTTACTACTATGGGGAGCATTCAATCTGGCTTTGTGGGGCTTAGCAAAGCGAGAAGCACAAAAAGAA
GAAAATAAAGAAGCGTATGAAGCTGCGCAATCTGAAATGGCTTTTATTCGTGAACACAGCACCATTCTAAAAACAAAACT
TGAAGCTGCAATTGCAACAATCAAGCGTGCTGGGCTATATGGAAAACTCAATAATAACGTTAAATATCAACTTCCTTGGT
ATATGGTTATTGGCCCACAAAATAGTGGTAAAACAACCCTTTTAGAGTGTTCAGGATTAGATTTCCCTTTAAACCAAACT
GATGGGCATTACACAAGAGACATTCAAAATAGCCAACATTGTGAATGGTATTTTGCTAACCACGCCGTTCTTTTAGATGC
TGCAGGACGCTTTTTTGATCAACATGAAAATGAGTTAAGTAAACCAATTTGGGGGAATTTTTTAAAAGCTTTACGCAATA
AGCGTCGTCGTCGCCCATTAAATGGGGTCATACTAACTATTGATATCACAACATTACAATCTCCAGATGAAAGCGCTATC
GAAGTTCAAGCTCGCTATGTACGTGAACGCTTACAAGAGTTAAGACATGATTTAAGCTCAGATATGCCTATCTATTTCTT
ATTAACGAAAATGGATAAAGTGGAAGGATTCGAACCCTTCTTCTCTTCCCTATCCAGAGAAGAAATGGACCAAGTGTTTG
GCGTCACTTTTAACGAAGGTGAAGGCCAACAAGCCGATAAAATTAAGCATGAGTTCGAGAAACTCATTTTACGTATCGAT
TCGCAAGTCATGTCACGCTTACATGATGAGCGTGATGTGATGAAACGTGCAGAACTATTACAATTCCCACGCCAATTAAG
CTTCCTTGCTGAACGCTTAGCGTTATTTTCTGAATTAGCCTTTACTAAAACCCGTTATCACCAAGCTTCTCATTTACGTG
GTTTATACCTAACGTCAGTACCCGAACCAACAAATACCCAGACTGAATCAACCACGTCAGGTATTGGGCAAAACCTTGGT
ATCAGCTCTCGAGTTCTTCCTACTTATAAACATCAACGAGGGTTCTTTATTCGAAAATTACTTGAAGAGGTTATTTTTCC
AAACAGTGAACTCGCTACATTAGATGAAAAATATGAGAAACAAGTAAAACTGAAAAATATGGCGGTCTATGCTGCCTCTT
TTGCAATTGTAATTGGATTTGGTTCATTATGGGCAACCAGCTTTTTAACCAATCATCATAAGCAACAAGAGTTGCTGTCT
CTTTCTAGTGATTATAATCAACAGATTGTCCATTTATCCCCTCTAGCTCCTTCTATTGAATTACTGCCAACGTTAAATAC
CTTATTAGCAGCAACTCAAGTGTATACAAAAGAGGGAGAAACGCTCACAGATGAGTTTGCGGGACTCCAACAAGGAGATA
AATTACGAAAGGCAGCAGAAGCTGCATACCACAGAAGTTTACTCAGTTTACTGCTTCCTAAAGTAACAAGTGAACTCGAA
TACCAAGTGGCAAACAATCAAGATAATAGAGAATTTTTAACGCCTTCTTTACGTGCATATTTGATGCTAAATCTACATGA
GCACCTTGATAAAGCTTATCTAGAACAATGGATGGGATTGCATTGGTCGTATTTATACAGTGGTTCAGCAACAGAGCAGA
AAGAGCTGCAAAATCACTTTTCAAATTTATTAGCTATTGAATTTGATCCTGTTCCACTGAACAATAATTTAATCGCTAAA
TCTCGTAAATTTTTACGTGAAGCCAATACATCAGAATTGGTATACCAACAGCTAAAACAAGACGCAAAAGAGATGGATCT
GCGTGATATAAAAATCAGTGACCATTTAGGACCAAACCAAAACTTATTCAACCACACCAATACCATTATTCCTGGTTTAT
ATACGCAAAAAGGATATAAAGCTGTCTTTTTAAGCAAAGGATTAGACCAAGTAAAACAACTGATTGAAGAAAACTGGGTT
ATTGGACTATCCAGTGATTTAAGTGCTAATGAGATCCGTGGACTATACGCAGATGTCGAAGATCTATATTTTCATGATTA
CATTAAATATTGGAAAGAAGCGGTAGATCAACTTCAAATTAAACCATCTAAAAACATGGATGAAGCCATTCTTCAAATTT
CAAATATTACCGGTTCAAGCCAACCAATTTTAAAGCTTTTAAACCTAGTAAAAGAAAATACAACCTTTGTTGATAAGGCT
CAAGTGGCTTCAAAAACTGCAGGTCAGGTTAAGAAACTACCTGTAAATACGAAACTAAAGAAAGTCGCCTCTCTGGGCTT
ACACAGTGTCGAAGAACGCTCTCAAAGCGCTCGTAAAGCGGTAAGCAATCAATTTGAACCACTGAACCTGTTACTCACAG
ATAAAGATCAAGCAACGATTCCATTAGAAGATGCGCTAGTAGTGATCAACGAACTGAGTGGACGATTATCTATGGTGAAG
TTCTCACCTAACCCTGATTCATCAGCCTTTAAGATTGCTCGCGATCGCATGCAAGGAATACCAAATGAGTTAAATGATAT
ACGTGTAATGGCAGAAACGCTTCCCCAACCATTACAGCAATGGTGGAAACAAATATCAAATAACGCTTGGGGTATTATAC
TGAACCATTCAAGAAAACATGTACAAATGGTTTACCATGATAAAGTCGTTACTCATTACGATGTCGCTTTATCAGGTCGA
TACCCATTTAGAAACAGTAGTCATGATGTAAACATTGCTGACTTTGATGAATTCTTCCAAAACGGCGGTATTTTAGACAA
TTTCTTTGACGCCTATTTATCCTCATTTGTTTATAAACAAAAAGGGAAATTCAAACAAAAGTCCTTACACGGACGTTCAT
TAGGGCTATCTGATTCATTCTTAGACCAATATAACTATGGATTGACTATCCAACGTATGTTTTATGGTTCAAAAGGAAAA
GAAGCCAACGTCTCGTTCCGTATTGCGCCATTTGAACTTGATGCAAGCGCTTTGCAATCCACTTTCTATTACGGAAAAAA
CAAATTTGCTTACCGCCATGGTCCAATACAGAAAAAACAATTTACGTGGCCAATAACCAATCAGCGTCAATACGTCAGTT
TTGTTTTACAAGATCTAAGTGGATCTAAAGTCGTAAACCAACAAAACTCTGGCCCTTGGGCTTTCTTCCGAGTATTAGAC
AAATTCAGAGTCAATAAATACCGAGGTCAAGATGTCATAAAAGTAGATCTGGAAAATAAAGGAATGAAAGCCAAATACCT
TATTTATAGTGATCGAACCCCTAACCCTTTTAATAGAAAAATATTAACGAATTTCTATCTTCCAAAAAGGATTAATGGAT
GA					