Detailed information of component protein


Summary

Component ID T6CP015838
Component protein type VasK
T6SS ID (Type) T6SS01274 experimental (Type i2)
Strain Klebsiella pneumoniae subsp. pneumoniae ATCC 43816
Replicon chromosome
Sequence Protein sequence (1150 a.a.); Nucleotide sequence (3450 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) IT767_RS08535
Coordinate (Strand) 1764049..1767498 (-)
NCBI ID WP_038431759.1
RefSeq NZ_CP064352
Uniprot ID UPI0005028CC5
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 4e-58 423..740
IcmF_C PF06744.14 1.3e-21 999..1103
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 METDKDKNQTQE
Transmembrane helix 13-35 ISAGITVLLIAVAVTLVIMLGGF
Outside 36-49 AYWLIAGERSTEWS
Transmembrane helix 50-81 VISPVLLVCSLLWVTLACVIALAFLAVHFWII
Inside 82-374 SRVKRTTAISQTNEAKKKARERRLTLARDIGTALRKRYSLFWHRKVRLLLVTGDEAAIEQLVPGLRQQRWLEGQRTVLIYGGSLLSEPDSEQYAALRKLRRGRPLDGIVRVMPSSLTLTPQISESDLHGLEKISELLGYAAPVWLWKLCDSEWPQADRAVQAVGVSFPLRATEEDVARQLAQMLPTLREQGMHQIAADTRHDFLLRLGQQLIDGEIAQWRRQLAPWLTASRQRLALRGLMFSLPEPRTVDPYQEADALPASQPHLLTLPATWQGIVDDCRRLRGHHVGMAWER
Transmembrane helix 375-397 GLACGLLAIFGLWAAGLLLSFAL
Outside 398-1149 NYSQIASVAGKARDLVAHPSVSDYQLTALHALRNEASRLVHDGQNGAPWYRRFGLDHHQQLLDAVLPWYGVANHRLIRDPANAALQQALNALVNSAPNSDLRAQLAKPGYDQLKAWLMMARPDKADGTFFAQTMKTVQPTRMGISTGLWQSLAPDLWAFYLSLLPERPEWKIIPDAQRMSQSRQVLLQQLGRRNAESTLYENMLKSVRRNFADVSLEDMTSSTDARRLFTTDEVVPGMFTRQAWEGGIQQAIDKAASSRREEIDWVLSDSRKTMSTDLSPEALKARLTRRYFTDFAGSWLNFLNSLRLNPATTIADVTDQLTLISDVRQSPLIALMNTLAWQGQAGQQREGLSDSLIKSAKDLVGGKDKPVIDQSAAGPQGPLDDTFGPLLQLMGKNTGSNVMSADSTLSLQTYLTRITRVRLRLQQVANASDPQEMMQTLAQTVFQGKSVDLTDTQQYGSLISASLGEEWSGFGNTLFVQPLTQAWETVLLPSAASLNDKWRRSVVANWHTAFDGRFPFAASKSDASLPMLAEFVRKDSGRIERFLTTELNGVLHKEGSQWVPDKVNSHGLVFNPAFLRAINQLSQLSDILFTDGSQGISFELQARPAPEVVETQLTIDGQKLRYFNQMADWQTFRWPGETYKPGTLLTWTTVNAGTRLFGDYSGTWGFIRWLEQGKRHPLDRSQWMMSFSAPDGRTLQWVLRSQLGSGPLALLALRGLTLPDQIFTVDAAESAQVLTTGVGNSDMDEMEL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9982 - -


  Protein sequence: 1150 a.a.    

>T6CP015838 NZ_CP064352:c1767498-1764049 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816] [VasK]
METDKDKNQTQEISAGITVLLIAVAVTLVIMLGGFAYWLIAGERSTEWSVISPVLLVCSLLWVTLACVIALAFLAVHFWI
ISRVKRTTAISQTNEAKKKARERRLTLARDIGTALRKRYSLFWHRKVRLLLVTGDEAAIEQLVPGLRQQRWLEGQRTVLI
YGGSLLSEPDSEQYAALRKLRRGRPLDGIVRVMPSSLTLTPQISESDLHGLEKISELLGYAAPVWLWKLCDSEWPQADRA
VQAVGVSFPLRATEEDVARQLAQMLPTLREQGMHQIAADTRHDFLLRLGQQLIDGEIAQWRRQLAPWLTASRQRLALRGL
MFSLPEPRTVDPYQEADALPASQPHLLTLPATWQGIVDDCRRLRGHHVGMAWERGLACGLLAIFGLWAAGLLLSFALNYS
QIASVAGKARDLVAHPSVSDYQLTALHALRNEASRLVHDGQNGAPWYRRFGLDHHQQLLDAVLPWYGVANHRLIRDPANA
ALQQALNALVNSAPNSDLRAQLAKPGYDQLKAWLMMARPDKADGTFFAQTMKTVQPTRMGISTGLWQSLAPDLWAFYLSL
LPERPEWKIIPDAQRMSQSRQVLLQQLGRRNAESTLYENMLKSVRRNFADVSLEDMTSSTDARRLFTTDEVVPGMFTRQA
WEGGIQQAIDKAASSRREEIDWVLSDSRKTMSTDLSPEALKARLTRRYFTDFAGSWLNFLNSLRLNPATTIADVTDQLTL
ISDVRQSPLIALMNTLAWQGQAGQQREGLSDSLIKSAKDLVGGKDKPVIDQSAAGPQGPLDDTFGPLLQLMGKNTGSNVM
SADSTLSLQTYLTRITRVRLRLQQVANASDPQEMMQTLAQTVFQGKSVDLTDTQQYGSLISASLGEEWSGFGNTLFVQPL
TQAWETVLLPSAASLNDKWRRSVVANWHTAFDGRFPFAASKSDASLPMLAEFVRKDSGRIERFLTTELNGVLHKEGSQWV
PDKVNSHGLVFNPAFLRAINQLSQLSDILFTDGSQGISFELQARPAPEVVETQLTIDGQKLRYFNQMADWQTFRWPGETY
KPGTLLTWTTVNAGTRLFGDYSGTWGFIRWLEQGKRHPLDRSQWMMSFSAPDGRTLQWVLRSQLGSGPLALLALRGLTLP
DQIFTVDAAESAQVLTTGVGNSDMDEMEL*

  Nucleotide sequence: 3450 bp    

>T6CP015838 NZ_CP064352:c1767498-1764049 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816] [VasK]
ATGGAAACTGATAAGGACAAAAACCAGACCCAGGAAATTTCTGCCGGGATCACTGTACTGCTTATTGCTGTGGCAGTAAC
GCTGGTGATCATGCTGGGGGGATTTGCATACTGGTTGATTGCCGGAGAGCGTTCGACGGAATGGTCAGTGATTTCTCCAG
TTCTGCTTGTGTGCAGCCTCCTCTGGGTCACACTTGCCTGTGTGATCGCCCTGGCTTTCCTTGCCGTTCATTTTTGGATC
ATCAGTCGGGTCAAACGAACGACTGCAATATCACAGACAAATGAAGCCAAAAAGAAGGCCAGGGAAAGGCGCCTGACGCT
CGCCAGAGACATTGGCACCGCGCTGCGCAAACGCTACTCCCTCTTCTGGCACCGCAAAGTCCGCCTGCTGCTGGTTACCG
GCGACGAGGCGGCAATCGAACAACTGGTGCCAGGCCTGCGACAGCAGCGCTGGCTGGAGGGCCAGCGTACCGTTCTGATT
TACGGCGGCAGTCTGCTCTCCGAGCCCGACAGTGAGCAATATGCCGCGCTGCGCAAGCTGCGCCGTGGCCGTCCTCTGGA
CGGGATTGTGCGGGTGATGCCGTCGTCTCTCACCCTGACGCCGCAGATAAGCGAGAGCGACCTGCACGGGCTGGAAAAAA
TCAGCGAACTGCTGGGCTATGCCGCCCCGGTCTGGTTATGGAAACTGTGCGACAGTGAGTGGCCGCAGGCTGACCGCGCG
GTGCAGGCGGTAGGGGTTAGCTTCCCGTTGCGCGCCACTGAGGAAGATGTCGCCCGTCAGCTTGCGCAGATGTTGCCGAC
GCTACGGGAGCAGGGGATGCACCAGATCGCAGCAGATACCCGCCACGACTTCCTGCTGCGTCTGGGGCAGCAGCTTATCG
ACGGGGAGATCGCACAATGGCGCCGGCAGCTGGCGCCGTGGCTGACCGCCTCGCGGCAGCGGCTTGCGCTGCGAGGCCTG
ATGTTTAGCCTGCCAGAGCCTCGTACCGTAGATCCGTATCAGGAAGCTGATGCCCTGCCTGCGAGCCAGCCTCACCTCCT
GACGCTGCCCGCCACCTGGCAGGGGATCGTCGATGACTGCCGCCGTTTACGCGGGCATCACGTGGGAATGGCATGGGAGC
GGGGCCTGGCGTGCGGTCTGCTGGCCATCTTCGGCCTGTGGGCGGCGGGACTGCTGCTCTCCTTTGCCCTCAATTACAGT
CAGATTGCTTCGGTAGCGGGAAAAGCCCGCGACCTGGTGGCCCACCCCTCCGTTTCTGACTATCAACTGACGGCCCTCCA
TGCCCTGCGCAATGAGGCCAGCCGTCTCGTGCACGATGGGCAGAATGGCGCGCCCTGGTATCGGCGCTTTGGTCTGGACC
ATCATCAGCAACTATTAGACGCCGTCCTGCCCTGGTACGGTGTGGCGAATCATCGCCTGATACGTGACCCGGCCAATGCC
GCGTTGCAGCAGGCGCTCAACGCGCTGGTCAACTCCGCCCCCAATAGCGACCTACGAGCCCAGCTGGCGAAACCGGGCTA
TGACCAGCTGAAAGCCTGGCTGATGATGGCTCGTCCGGATAAAGCCGATGGCACATTTTTCGCACAGACCATGAAAACGG
TCCAGCCGACGCGAATGGGGATCTCGACCGGTCTGTGGCAAAGTCTGGCGCCGGATTTGTGGGCCTTTTATCTCTCCCTG
CTGCCCGAGCGGCCAGAGTGGAAGATCATCCCGGATGCGCAACGGATGAGTCAGAGCCGTCAGGTTCTGTTGCAGCAGCT
AGGTCGCCGCAATGCCGAAAGCACCTTATATGAGAACATGCTCAAATCCGTGCGTCGTAATTTTGCCGATGTATCGCTGG
AAGACATGACCAGTAGCACCGACGCGCGCCGCCTGTTTACCACCGATGAAGTGGTCCCGGGGATGTTTACCCGCCAGGCA
TGGGAAGGTGGCATTCAGCAGGCCATTGATAAGGCAGCCAGCTCGCGCCGGGAGGAAATCGACTGGGTGCTGAGCGATTC
GCGCAAAACGATGTCGACAGACCTGTCGCCGGAGGCGCTGAAAGCGCGTCTGACCCGACGCTACTTCACCGATTTCGCCG
GCAGCTGGCTGAATTTTCTCAACAGTCTGCGACTTAATCCGGCGACCACCATTGCCGATGTTACCGACCAGCTCACGCTG
ATAAGCGATGTCCGCCAGTCGCCGCTGATTGCCCTGATGAATACCCTTGCCTGGCAGGGGCAGGCGGGGCAACAAAGGGA
GGGGCTGTCGGACAGCCTTATTAAATCGGCCAAAGATCTGGTGGGCGGGAAAGACAAGCCGGTCATCGACCAGTCGGCAG
CCGGCCCGCAGGGGCCTCTGGATGACACGTTCGGCCCACTGCTCCAGCTGATGGGGAAAAACACGGGCAGCAACGTCATG
TCGGCCGATAGCACCCTGAGCCTGCAAACCTATCTGACCCGTATCACCCGCGTGCGCCTGCGCCTGCAGCAGGTTGCGAA
TGCTTCCGATCCGCAGGAGATGATGCAGACCCTGGCGCAGACCGTCTTCCAGGGGAAAAGCGTCGACCTGACCGACACGC
AACAGTACGGTAGCCTGATTTCGGCAAGCCTTGGCGAAGAGTGGAGTGGCTTCGGTAATACTCTGTTTGTGCAGCCGCTG
ACTCAGGCCTGGGAAACCGTGCTTCTGCCGTCGGCGGCCAGTCTGAATGATAAATGGCGCCGCTCGGTGGTCGCCAACTG
GCACACCGCCTTTGACGGACGCTTTCCGTTTGCCGCCAGCAAAAGCGATGCCTCCCTGCCGATGCTGGCTGAGTTTGTGC
GCAAAGACAGCGGGCGAATCGAACGTTTTCTCACGACGGAGCTGAACGGTGTCCTGCATAAAGAAGGCAGTCAATGGGTG
CCGGATAAGGTCAACAGTCACGGCCTGGTCTTTAATCCGGCCTTTCTGCGCGCGATCAACCAGCTAAGCCAGCTGTCCGA
CATTCTTTTCACCGACGGCAGCCAGGGGATCAGCTTTGAGTTACAGGCCAGGCCTGCCCCGGAGGTTGTCGAGACACAGC
TGACGATAGACGGCCAAAAGCTGCGCTATTTCAATCAAATGGCCGACTGGCAGACTTTCCGCTGGCCGGGGGAGACGTAT
AAACCCGGCACTCTGCTTACCTGGACCACCGTCAATGCGGGCACGCGCCTGTTCGGTGACTACAGCGGTACCTGGGGTTT
TATTCGCTGGCTGGAGCAGGGTAAACGCCATCCGCTGGATCGCAGCCAGTGGATGATGAGCTTCTCCGCCCCGGATGGCC
GCACTCTGCAGTGGGTATTGCGCTCCCAGCTTGGGAGCGGCCCGCTGGCGCTTCTGGCGCTGCGCGGATTGACCCTGCCG
GATCAGATTTTTACTGTCGATGCGGCTGAGAGCGCACAGGTCCTGACGACGGGGGTAGGGAATAGTGACATGGATGAGAT
GGAATTATGA