SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015835
Component protein type	TssG
T6SS ID (Type)	T6SS01274 (Type i2)
Strain	Klebsiella pneumoniae subsp. pneumoniae ATCC 43816
Replicon	chromosome
Sequence	Protein sequence (362 a.a.); Nucleotide sequence (1086 bp)
Reference	[1] Storey D, McNally A, Åstrand M, et al. Klebsiella pneumoniae type VI secretion system-mediated microbial competition is PhoPQ controlled and reactive oxygen species dependent. PLoS Pathog. 2020 Mar 19;16(3):e1007969. doi: 10.1371/journal.ppat.1007969. eCollection 2020 Mar. PMID: 32191774

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	IT767_RS08520
Coordinate (Strand)	1759577..1760662 (-)
NCBI ID	WP_004212345.1
RefSeq	NZ_CP064352
Uniprot ID	A0A2L1BZK9_KLEPN, A0A7M3G8Y6_KLEPN
KEGG ID	kpt:VK055_1090
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	5.7e-63	21..320

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-361	MGREAQPPHSRLTPRLEADLPRINFYRFCQLLEKRRPGQPLMGGTSHPADDPVRFYPHPGMGFPASELKAVEYDEADDSRPPVIRTTFMGLYGVDSPLPTAYLDDIAQHREGHEALQGLLDIFSHRIMTQFYRIWRKYSWPATFEPGGTDRLSQSLLGLAGLGIPGTTQHIASPASRFLALTGVLRQPGKTQQGIQALVTLLAPETTVRVSPYSLRPVAISQPLGFYGDDDFFLDGNTPLGDEAMDASSQLLVALTTDNPAEAQGWKPDGPLFRDFLILLRVYLGWRFRANITLTAPTRLLAVPPLGDEPFWLGMNGVLGVGQGESEDDIPQTFTTELGTYTGLQPATFLQGNRRVTYKFD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9812	-	-

Protein sequence: 362 a.a.

						>T6CP015835 NZ_CP064352:c1760662-1759577 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816] [TssG]
MGREAQPPHSRLTPRLEADLPRINFYRFCQLLEKRRPGQPLMGGTSHPADDPVRFYPHPGMGFPASELKAVEYDEADDSR
PPVIRTTFMGLYGVDSPLPTAYLDDIAQHREGHEALQGLLDIFSHRIMTQFYRIWRKYSWPATFEPGGTDRLSQSLLGLA
GLGIPGTTQHIASPASRFLALTGVLRQPGKTQQGIQALVTLLAPETTVRVSPYSLRPVAISQPLGFYGDDDFFLDGNTPL
GDEAMDASSQLLVALTTDNPAEAQGWKPDGPLFRDFLILLRVYLGWRFRANITLTAPTRLLAVPPLGDEPFWLGMNGVLG
VGQGESEDDIPQTFTTELGTYTGLQPATFLQGNRRVTYKFD*					

Nucleotide sequence: 1086 bp

						>T6CP015835 NZ_CP064352:c1760662-1759577 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816] [TssG]
ATGGGAAGAGAAGCACAGCCGCCGCATTCCCGGCTGACCCCGCGACTGGAGGCCGACCTGCCGCGGATCAATTTTTACCG
CTTCTGCCAGCTTCTGGAAAAACGGCGTCCCGGCCAACCGCTGATGGGGGGAACCAGCCATCCGGCTGACGATCCGGTGC
GTTTTTACCCGCATCCGGGAATGGGATTTCCCGCCAGCGAACTGAAAGCCGTGGAATACGATGAGGCCGATGACAGCAGG
CCGCCGGTCATTCGCACCACCTTTATGGGGTTGTACGGTGTCGATTCGCCGCTCCCGACGGCATATCTCGATGATATCGC
CCAGCACAGGGAAGGGCATGAAGCGCTGCAGGGCTTACTGGATATCTTCAGCCACCGGATCATGACGCAGTTTTACCGCA
TCTGGCGGAAATACTCCTGGCCCGCCACCTTTGAGCCCGGCGGCACCGATCGTCTCTCGCAGTCGCTACTGGGCCTGGCG
GGGCTAGGCATCCCCGGCACGACGCAGCATATCGCCTCGCCGGCGTCGCGCTTTCTGGCGCTAACCGGCGTGCTGCGTCA
GCCGGGCAAAACCCAGCAGGGGATCCAGGCGCTGGTCACCCTGCTGGCCCCGGAGACCACCGTCAGGGTGAGCCCGTACA
GCCTGCGTCCGGTGGCGATCAGCCAGCCGCTGGGGTTTTACGGCGATGACGACTTCTTCCTGGACGGCAATACGCCGCTG
GGTGACGAAGCGATGGATGCCAGCAGCCAGCTCCTCGTCGCCCTTACCACCGACAATCCCGCGGAGGCGCAGGGCTGGAA
GCCTGACGGCCCGCTGTTTCGGGACTTTCTCATCCTGCTGCGGGTATACCTCGGCTGGCGATTCCGGGCCAACATCACCC
TGACCGCGCCGACCCGACTGCTGGCTGTCCCGCCGCTGGGGGACGAGCCGTTCTGGCTCGGTATGAACGGCGTGCTGGGC
GTGGGTCAGGGCGAGAGTGAGGACGATATTCCTCAGACCTTTACCACCGAGCTGGGTACCTATACGGGCCTGCAGCCTGC
CACCTTTCTACAAGGAAACCGACGTGTTACGTATAAGTTTGACTAA					