Detailed information of component protein
Summary
| Component ID | T6CP015831 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS01273  (Type Unknown) |
| Strain | Klebsiella pneumoniae subsp. pneumoniae ATCC 43816 |
| Replicon | chromosome |
| Sequence | Protein sequence (179 a.a.); Nucleotide sequence (537 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | IT767_RS04075 |
| Coordinate (Strand) | 876980..877516 (+) |
| NCBI ID | WP_004175532.1 |
| RefSeq | NZ_CP064352 |
| Uniprot ID | UPI0001576BE4 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 1e-32 | 47..167 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-178 | MAITAVKPSARLLAFLMVTVLTGCGLTQTVKDGAVSVTQSIFYPQVKTLHLDLRAREGVNNNAKGASLATVVRIYQLKDRQAFDNTDYPSLFAGDGQALQADRVAEKDVRLRPGESVTVDMPMETSAQFVAVAAMFIDPDLTQNSWRLVLTRDELDPARPRIIEASQNQLTLHPFKEK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9977 | pos: 23-24. LTG-CG. | MAITAVKPSARLLAFLMVTVLTG |
Protein sequence: 179 a.a.
>T6CP015831 NZ_CP064352:876980-877516 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816] [TssJ]
MAITAVKPSARLLAFLMVTVLTGCGLTQTVKDGAVSVTQSIFYPQVKTLHLDLRAREGVNNNAKGASLATVVRIYQLKDR
QAFDNTDYPSLFAGDGQALQADRVAEKDVRLRPGESVTVDMPMETSAQFVAVAAMFIDPDLTQNSWRLVLTRDELDPARP
RIIEASQNQLTLHPFKEK*
MAITAVKPSARLLAFLMVTVLTGCGLTQTVKDGAVSVTQSIFYPQVKTLHLDLRAREGVNNNAKGASLATVVRIYQLKDR
QAFDNTDYPSLFAGDGQALQADRVAEKDVRLRPGESVTVDMPMETSAQFVAVAAMFIDPDLTQNSWRLVLTRDELDPARP
RIIEASQNQLTLHPFKEK*
Nucleotide sequence: 537 bp
>T6CP015831 NZ_CP064352:876980-877516 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816] [TssJ]
ATGGCGATTACCGCAGTTAAACCCTCCGCCAGGTTGCTGGCATTCCTCATGGTTACCGTCTTAACCGGCTGCGGACTGAC
GCAGACCGTGAAAGACGGCGCCGTTTCCGTGACCCAGTCCATTTTTTACCCCCAGGTGAAAACCCTGCATCTGGATCTTC
GCGCCCGGGAAGGCGTGAATAACAACGCCAAAGGCGCCTCCCTGGCGACGGTGGTGCGTATTTATCAGCTGAAGGACCGG
CAAGCGTTCGACAATACTGACTACCCGTCGCTGTTTGCCGGCGATGGCCAGGCGCTGCAGGCGGACCGGGTGGCGGAAAA
AGATGTTCGCCTGCGTCCGGGCGAATCGGTGACCGTCGATATGCCCATGGAAACCAGCGCGCAGTTTGTGGCGGTGGCGG
CCATGTTTATCGATCCAGACCTGACGCAAAACAGCTGGCGGCTGGTACTCACCCGGGACGAACTTGATCCGGCGAGGCCG
CGCATTATCGAAGCCAGCCAAAATCAACTGACGCTGCACCCGTTTAAGGAGAAGTGA
ATGGCGATTACCGCAGTTAAACCCTCCGCCAGGTTGCTGGCATTCCTCATGGTTACCGTCTTAACCGGCTGCGGACTGAC
GCAGACCGTGAAAGACGGCGCCGTTTCCGTGACCCAGTCCATTTTTTACCCCCAGGTGAAAACCCTGCATCTGGATCTTC
GCGCCCGGGAAGGCGTGAATAACAACGCCAAAGGCGCCTCCCTGGCGACGGTGGTGCGTATTTATCAGCTGAAGGACCGG
CAAGCGTTCGACAATACTGACTACCCGTCGCTGTTTGCCGGCGATGGCCAGGCGCTGCAGGCGGACCGGGTGGCGGAAAA
AGATGTTCGCCTGCGTCCGGGCGAATCGGTGACCGTCGATATGCCCATGGAAACCAGCGCGCAGTTTGTGGCGGTGGCGG
CCATGTTTATCGATCCAGACCTGACGCAAAACAGCTGGCGGCTGGTACTCACCCGGGACGAACTTGATCCGGCGAGGCCG
CGCATTATCGAAGCCAGCCAAAATCAACTGACGCTGCACCCGTTTAAGGAGAAGTGA