SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015764
Component protein type	TssH
T6SS ID (Type)	T6SS01265 (Type i4b)
Strain	Burkholderia glumae 336gr-1
Replicon	chromosome
Sequence	Protein sequence (705 a.a.); Nucleotide sequence (2115 bp)
Reference	[1] Peng J, Lelis T, Chen R, et al. tepR encoding a bacterial enhancer-binding protein orchestrates the virulence and interspecies competition of Burkholderia glumae through qsmR and a type VI secretion system. Mol Plant Pathol. 2020 Aug;21(8):1042-1054. doi: 10.1111/mpp.12947. Epub 2020 Jul 1. PMID: 32608174

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	6047..8161 (+)
NCBI ID	-
GenBank	AYPW01000191
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
AAA_lid_9	PF17871.3	3.6e-28	374..463
AAA_2	PF07724.16	2.1e-21	619..705
Clp_N	PF02861.22	1.3e-09	23..74
AAA	PF00004.31	5.9e-09	232..344

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-705	MSTPLKTLIAKLNPTCRKAAERAASQCLARGHYEVDLEHLFVALLDETSGDMPVVLRASGVDPHALRRDLERELDTLKSGNTRTPVFSVHLSELFQQAWLIASLDAQIGRIRSGHLLLALLSAPDLAQFAQRMSPQFAQVRVTDLKHKFDEITAGSHEAEPRGAEAGHDGAPEAAALAGAAGGPSKTPALDTYTTNLTQRARDGKIDPVIGRDAEIRQAIDILMRRRQNNPIMTGEAGVGKTAVVEGLALRIAAADVPPPLAGVALHVLDMGLLQAGASVKGEFENRLKSVIDEVKQSPHPIILFIDEAHTIIGAGGQAGQNDAANLLKPALARGELRTIAATTWSEYKKYFEKDAALARRFQVVKIEEPSEPLAAAMLRGMAALMERHFNVRILDDAITEAVRLSHRYISGRQLPDKAISVLDTACAKVALAQSATPAAIDDLKKRLERTAAEIAALEREVAGGAAHDERLAALGAARDADLAALAAAEARYAEEQAIVAEIVALRAQIDASREASADGERIDADAVRAQLAERVAALKAVQGGEPMVPLQVDAHVVAEIVAAWTGIPLGRMVKDEIETVMNLKALLGARVIGQDHALDAVAQRVRTATAGLEDPNKPRGVFLFVGPSGVGKTETALALADVLYGGERKMVTINMSEYQEAHSVSGLKGSPPGYVGYGEGGVLTEAVRRNPYSVVLLDEIEKAH

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9905	-	-

Protein sequence: 705 a.a.

						>T6CP015764 AYPW01000191:6047-8161 [Burkholderia glumae 336gr-1] [TssH]
MSTPLKTLIAKLNPTCRKAAERAASQCLARGHYEVDLEHLFVALLDETSGDMPVVLRASGVDPHALRRDLERELDTLKSG
NTRTPVFSVHLSELFQQAWLIASLDAQIGRIRSGHLLLALLSAPDLAQFAQRMSPQFAQVRVTDLKHKFDEITAGSHEAE
PRGAEAGHDGAPEAAALAGAAGGPSKTPALDTYTTNLTQRARDGKIDPVIGRDAEIRQAIDILMRRRQNNPIMTGEAGVG
KTAVVEGLALRIAAADVPPPLAGVALHVLDMGLLQAGASVKGEFENRLKSVIDEVKQSPHPIILFIDEAHTIIGAGGQAG
QNDAANLLKPALARGELRTIAATTWSEYKKYFEKDAALARRFQVVKIEEPSEPLAAAMLRGMAALMERHFNVRILDDAIT
EAVRLSHRYISGRQLPDKAISVLDTACAKVALAQSATPAAIDDLKKRLERTAAEIAALEREVAGGAAHDERLAALGAARD
ADLAALAAAEARYAEEQAIVAEIVALRAQIDASREASADGERIDADAVRAQLAERVAALKAVQGGEPMVPLQVDAHVVAE
IVAAWTGIPLGRMVKDEIETVMNLKALLGARVIGQDHALDAVAQRVRTATAGLEDPNKPRGVFLFVGPSGVGKTETALAL
ADVLYGGERKMVTINMSEYQEAHSVSGLKGSPPGYVGYGEGGVLTEAVRRNPYSVVLLDEIEKAH					

Nucleotide sequence: 2115 bp

						>T6CP015764 AYPW01000191:6047-8161 [Burkholderia glumae 336gr-1] [TssH]
ATGAGCACGCCCCTCAAAACCCTGATTGCCAAACTGAATCCCACCTGCCGCAAGGCGGCCGAACGCGCGGCCAGCCAGTG
CCTCGCGCGCGGCCACTACGAGGTCGATCTCGAGCACCTGTTCGTGGCGCTGCTCGACGAGACCTCGGGCGACATGCCGG
TGGTGCTGCGCGCGAGCGGCGTCGATCCGCACGCGCTGCGCCGCGACCTCGAGCGCGAGCTCGACACGCTCAAGAGCGGC
AACACGCGCACGCCGGTGTTCTCGGTGCATCTGAGCGAGCTGTTCCAGCAGGCCTGGCTGATTGCCTCGCTCGATGCGCA
GATCGGCCGGATCCGTTCCGGGCACCTGCTGCTCGCGCTGCTGAGCGCGCCGGACCTCGCGCAGTTCGCGCAGCGCATGT
CGCCGCAGTTCGCGCAGGTGCGCGTGACCGATCTGAAGCACAAGTTCGACGAGATCACGGCCGGCTCCCACGAGGCCGAG
CCGCGCGGCGCCGAGGCCGGGCACGACGGCGCGCCCGAGGCCGCCGCGCTGGCCGGTGCCGCGGGCGGCCCGTCGAAGAC
GCCCGCGCTCGACACCTACACCACCAACCTCACGCAGCGCGCGCGCGACGGCAAGATCGATCCGGTGATCGGCCGCGACG
CCGAGATCCGCCAGGCGATCGACATCCTGATGCGGCGCCGCCAGAACAACCCGATCATGACCGGCGAGGCCGGCGTCGGG
AAGACCGCCGTGGTGGAAGGGCTGGCGCTGCGGATCGCCGCGGCCGACGTGCCGCCGCCGCTCGCGGGCGTGGCGCTGCA
CGTGCTCGACATGGGGCTGCTGCAGGCCGGCGCGAGCGTGAAGGGCGAGTTCGAGAACCGCCTGAAGAGCGTGATCGACG
AGGTCAAGCAGAGCCCGCATCCGATCATCCTGTTCATCGACGAGGCGCACACCATCATCGGCGCCGGCGGCCAGGCCGGC
CAGAACGACGCGGCCAACCTGCTCAAGCCGGCGCTCGCGCGCGGCGAGCTGCGCACCATCGCGGCCACCACCTGGAGCGA
GTACAAGAAATACTTCGAGAAGGATGCGGCGCTCGCGCGGCGCTTCCAGGTGGTCAAGATCGAGGAGCCGAGCGAGCCGC
TGGCCGCCGCGATGCTGCGCGGCATGGCCGCGCTGATGGAGCGCCACTTCAACGTGCGGATTCTCGACGACGCGATCACC
GAGGCGGTGCGCCTGTCGCATCGCTACATCAGCGGCCGGCAGCTGCCGGACAAGGCGATCAGCGTGCTCGATACGGCCTG
CGCGAAGGTCGCGCTGGCGCAGAGCGCGACGCCGGCGGCCATCGACGACCTGAAGAAGCGCCTGGAGCGCACCGCCGCCG
AGATCGCCGCGCTCGAGCGCGAGGTGGCGGGCGGGGCCGCGCACGACGAGCGCCTCGCCGCGCTCGGCGCGGCGCGCGAC
GCCGATCTGGCCGCGCTGGCGGCGGCCGAGGCGCGCTATGCCGAGGAGCAGGCGATCGTCGCCGAGATCGTCGCGCTGCG
CGCGCAGATCGATGCCTCGCGCGAGGCATCGGCCGACGGCGAGCGGATCGACGCCGACGCGGTGCGCGCCCAGCTGGCCG
AGCGCGTGGCCGCGCTGAAGGCGGTGCAGGGCGGCGAGCCGATGGTGCCGCTGCAGGTGGACGCGCACGTGGTGGCCGAG
ATCGTCGCCGCGTGGACCGGCATCCCGCTCGGCCGCATGGTCAAGGACGAGATCGAGACCGTGATGAACCTGAAGGCGCT
GCTCGGCGCGCGCGTGATCGGTCAGGACCACGCGCTCGACGCGGTGGCGCAGCGCGTGCGCACCGCGACCGCCGGCCTCG
AGGATCCGAACAAGCCGCGCGGCGTGTTCCTGTTCGTCGGGCCGTCGGGCGTCGGCAAGACCGAGACCGCGCTCGCGCTG
GCCGACGTGCTGTACGGCGGCGAGCGCAAGATGGTCACCATCAACATGAGCGAGTACCAGGAGGCGCACAGCGTGTCGGG
CCTGAAGGGCTCGCCGCCGGGCTACGTCGGCTACGGCGAGGGCGGCGTGCTGACCGAGGCGGTGCGGCGCAACCCGTATT
CGGTGGTGCTGCTCGACGAGATCGAGAAGGCGCAC					