SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015756
Component protein type	TssJ
T6SS ID (Type)	T6SS01264 (Type i3)
Strain	Serratia marcescens RM66262
Replicon	chromosome
Sequence	Protein sequence (177 a.a.); Nucleotide sequence (531 bp)
Reference	[1] Lazzaro M, Feldman MF, García Véscovi E. A Transcriptional Regulatory Mechanism Finely Tunes the Firing of Type VI Secretion System in Response to Bacterial Enemies. mBio. 2017 Aug 22;8(4). pii: e00559-17. doi: 10.1128/mBio.00559-17. PMID: 28830939

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	RT90_RS02725
Coordinate (Strand)	591895..592425 (-)
NCBI ID	913459525
RefSeq	NZ_JWLO01000001
Uniprot ID	UPI00044D6217
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	1e-28	36..148

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-176	MMTTPIASRWCGMLFLLLSALALGGCMSSAKSVPSRYSLVFDADRQVNAAAGAQPAPIKIRVLLLRSDAEFMDADFFSLQNDAKSVLGNSLLDSDQFFLTPGQTGKKLGGQSALDARYIGVIAEYQNLDGKTWRLSLPLPEPTETNFYKVWQFSPDELEAHIVAGVSGLRPVKKVD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9874	pos: 25-26. LGG-CM.	MMTTPIASRWCGMLFLLLSALALGG

Protein sequence: 177 a.a.

						>T6CP015756 NZ_JWLO01000001:c592425-591895 [Serratia marcescens] [TssJ]
MMTTPIASRWCGMLFLLLSALALGGCMSSAKSVPSRYSLVFDADRQVNAAAGAQPAPIKIRVLLLRSDAEFMDADFFSLQ
NDAKSVLGNSLLDSDQFFLTPGQTGKKLGGQSALDARYIGVIAEYQNLDGKTWRLSLPLPEPTETNFYKVWQFSPDELEA
HIVAGVSGLRPVKKVD*					

Nucleotide sequence: 531 bp

						>T6CP015756 NZ_JWLO01000001:c592425-591895 [Serratia marcescens] [TssJ]
ATGATGACGACCCCTATCGCTTCCCGCTGGTGCGGGATGCTTTTTCTGCTGCTGAGCGCGTTGGCGCTCGGCGGCTGCAT
GTCTTCCGCCAAAAGCGTGCCGTCGCGATACAGCCTGGTGTTCGATGCCGATCGCCAGGTCAACGCCGCCGCCGGCGCGC
AACCGGCGCCGATAAAAATTCGCGTGCTGCTGCTGCGTTCGGATGCGGAATTCATGGATGCCGACTTCTTCAGTCTGCAA
AACGACGCCAAAAGCGTGCTCGGCAACAGCCTGCTGGACAGCGATCAGTTCTTCCTGACGCCGGGCCAGACCGGCAAAAA
ACTCGGTGGGCAAAGCGCGTTGGATGCGCGCTACATCGGCGTGATCGCCGAATATCAAAATCTGGACGGCAAAACCTGGC
GCCTTTCACTGCCGCTGCCGGAACCCACCGAAACCAATTTCTACAAGGTATGGCAATTCTCGCCGGATGAGCTGGAAGCG
CATATCGTCGCGGGCGTGAGTGGCCTGCGCCCCGTAAAAAAGGTCGACTGA					