SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015745
Component protein type	TssE
T6SS ID (Type)	T6SS01264 (Type i3)
Strain	Serratia marcescens RM66262
Replicon	chromosome
Sequence	Protein sequence (192 a.a.); Nucleotide sequence (576 bp)
Reference	[1] Lazzaro M, Feldman MF, García Véscovi E. A Transcriptional Regulatory Mechanism Finely Tunes the Firing of Type VI Secretion System in Response to Bacterial Enemies. mBio. 2017 Aug 22;8(4). pii: e00559-17. doi: 10.1128/mBio.00559-17. PMID: 28830939

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	RT90_RS02645
Coordinate (Strand)	573020..573595 (-)
NCBI ID	727162842
RefSeq	NZ_JWLO01000001
Uniprot ID	UPI0003DFEFE6
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
GPW_gp25	PF04965.16	1.5e-19	63..167

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-191	MNDKRPPHYDGGDLHRQGYRYRQDSERLTSRDKMQPVLLDMLTDDEPQKKQETQVRNLVSHSELRRRVLRDLQWLFNCVNSESNLDLGDFPQVRRSTLNYGIASLAGKRMSDIEWLDIQRALTESILHFEPRILPEGLQVRCISDTGSLELHNVLSIEIKGRLWCVPYPLEFLFRTDVDLENGHFDLKDIG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9900	-	-

Protein sequence: 192 a.a.

						>T6CP015745 NZ_JWLO01000001:c573595-573020 [Serratia marcescens] [TssE]
MNDKRPPHYDGGDLHRQGYRYRQDSERLTSRDKMQPVLLDMLTDDEPQKKQETQVRNLVSHSELRRRVLRDLQWLFNCVN
SESNLDLGDFPQVRRSTLNYGIASLAGKRMSDIEWLDIQRALTESILHFEPRILPEGLQVRCISDTGSLELHNVLSIEIK
GRLWCVPYPLEFLFRTDVDLENGHFDLKDIG*					

Nucleotide sequence: 576 bp

						>T6CP015745 NZ_JWLO01000001:c573595-573020 [Serratia marcescens] [TssE]
ATGAACGACAAGCGCCCCCCCCACTACGACGGCGGCGATCTGCACCGCCAGGGCTATCGCTATCGTCAGGACAGCGAGCG
CCTGACGTCGCGCGACAAAATGCAGCCGGTGCTGCTCGATATGTTGACCGACGACGAGCCGCAGAAAAAACAGGAGACGC
AGGTGCGCAACCTGGTGTCGCACAGCGAACTGCGCCGCCGGGTGCTGCGCGATCTGCAGTGGCTGTTCAACTGCGTCAAC
AGCGAATCCAATCTGGATCTCGGCGATTTTCCGCAGGTGCGGCGCTCCACCCTGAATTACGGCATCGCCTCACTGGCGGG
CAAGCGCATGTCGGACATCGAATGGCTCGACATTCAGCGCGCGCTGACCGAGTCCATTCTCCATTTCGAGCCGCGCATTT
TGCCCGAGGGGCTGCAGGTGCGCTGCATCTCGGACACCGGCTCGCTGGAGCTGCACAACGTGTTGTCGATCGAAATCAAG
GGCCGCCTGTGGTGCGTGCCCTATCCGCTGGAGTTTCTGTTCCGTACCGACGTGGATCTGGAAAACGGCCACTTCGATCT
GAAAGACATAGGGTAA					