SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015743
Component protein type	TssG
T6SS ID (Type)	T6SS01264 (Type i3)
Strain	Serratia marcescens RM66262
Replicon	chromosome
Sequence	Protein sequence (357 a.a.); Nucleotide sequence (1071 bp)
Reference	[1] Lazzaro M, Feldman MF, García Véscovi E. A Transcriptional Regulatory Mechanism Finely Tunes the Firing of Type VI Secretion System in Response to Bacterial Enemies. mBio. 2017 Aug 22;8(4). pii: e00559-17. doi: 10.1128/mBio.00559-17. PMID: 28830939

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	RT90_RS02635
Coordinate (Strand)	570062..571132 (-)
NCBI ID	727185942
RefSeq	NZ_JWLO01000001
Uniprot ID	UPI0003DFDA09
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	1.1e-100	33..333

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-356	MSAEMIATELAPPQINALYRLPDDFWPRLRSAPYKHDLFQLLRRIDAQGGQPYLLGRAPLPRHEMLRLGQEPSLSFAPSTLAQVAPRANSPLHDVSIFSFGLFGPNGPLPLHLTEYVRERVYHHQDTTLLAFTNLFHHRLTLLFYRAWADAQPTVSLDRPDNRRFDEYLSSLIGIGQPAQQERDSINAHAKHFMAGHLIRHSRDPEGLSKILQQYFKVPVRIVENVPHWLRVEPREQARLKAGRGAPRLGESAFLGIAVRDIQHKFRIELGPMPQQDYDRFLPGADLCRQLRDWVRQYLGIEFVWEVRLILAKEQAHGIQLGGAQRLGLSSWLADDRRQRDLDDLIFSPEPLENPF

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9958	-	-

Protein sequence: 357 a.a.

						>T6CP015743 NZ_JWLO01000001:c571132-570062 [Serratia marcescens] [TssG]
MSAEMIATELAPPQINALYRLPDDFWPRLRSAPYKHDLFQLLRRIDAQGGQPYLLGRAPLPRHEMLRLGQEPSLSFAPST
LAQVAPRANSPLHDVSIFSFGLFGPNGPLPLHLTEYVRERVYHHQDTTLLAFTNLFHHRLTLLFYRAWADAQPTVSLDRP
DNRRFDEYLSSLIGIGQPAQQERDSINAHAKHFMAGHLIRHSRDPEGLSKILQQYFKVPVRIVENVPHWLRVEPREQARL
KAGRGAPRLGESAFLGIAVRDIQHKFRIELGPMPQQDYDRFLPGADLCRQLRDWVRQYLGIEFVWEVRLILAKEQAHGIQ
LGGAQRLGLSSWLADDRRQRDLDDLIFSPEPLENPF*					

Nucleotide sequence: 1071 bp

						>T6CP015743 NZ_JWLO01000001:c571132-570062 [Serratia marcescens] [TssG]
ATGAGCGCGGAGATGATTGCCACCGAACTGGCGCCGCCGCAGATTAACGCGCTGTATCGCCTGCCGGACGACTTCTGGCC
GCGGCTGCGCAGCGCGCCTTACAAGCACGATCTGTTTCAGCTGCTGCGCCGCATCGACGCCCAGGGCGGCCAGCCTTACC
TACTGGGGCGCGCGCCGCTGCCGCGCCATGAGATGCTGCGTCTGGGCCAGGAGCCGTCGCTGTCGTTCGCGCCCTCCACG
CTGGCGCAGGTGGCGCCGCGCGCCAACAGCCCGCTGCACGACGTCTCGATCTTCAGCTTCGGTCTGTTCGGCCCCAACGG
GCCGCTGCCGCTGCACCTGACCGAATACGTGCGCGAACGGGTGTACCACCACCAGGACACCACCCTGCTGGCGTTCACCA
ACCTGTTCCATCACCGGCTGACGCTGCTGTTTTACCGCGCCTGGGCCGACGCGCAGCCGACGGTGTCGCTGGATCGCCCG
GACAACCGGCGCTTCGACGAGTACCTGTCGAGCCTGATCGGCATCGGCCAGCCGGCGCAGCAGGAACGCGACAGCATCAA
CGCCCACGCCAAACATTTTATGGCCGGTCATCTGATTCGCCACAGCCGCGATCCGGAAGGCCTGAGCAAGATTTTGCAGC
AGTATTTCAAGGTGCCGGTGCGCATCGTCGAAAACGTGCCGCACTGGCTGCGGGTGGAACCGCGCGAACAAGCTCGGCTC
AAGGCCGGGCGTGGTGCGCCGCGGCTCGGCGAGTCGGCCTTTCTCGGCATCGCGGTGCGCGATATTCAGCACAAATTCCG
CATCGAGCTGGGGCCGATGCCGCAGCAAGACTATGACCGCTTCCTGCCCGGCGCCGACCTGTGCCGGCAGCTGCGCGACT
GGGTGCGCCAATATCTGGGCATCGAGTTCGTCTGGGAAGTGCGCCTGATCCTGGCGAAGGAACAGGCGCACGGCATCCAG
CTCGGCGGCGCGCAGCGACTGGGCCTGAGCAGCTGGCTGGCCGACGATCGGCGGCAACGCGATCTCGACGATCTGATCTT
CAGCCCTGAACCGCTGGAAAATCCGTTCTGA					